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http://dx.doi.org/10.9799/ksfan.2011.24.3.291

Purification and Characterization of Lipase Inhibitor from Desmodium oxyphyllum DC.  

Lee, Jong-Kug (Chungnam Agricultural Research and Extension Services)
Kang, Min-Gu (Dept. of Life Science and Genetic Engineering, Paichai University)
Baek, Seung-Ye (Dept. of Life Science and Genetic Engineering, Paichai University)
Ahn, Young-Geun (Faculty of Food Nutrition, Chungcheong University)
Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
Publication Information
The Korean Journal of Food And Nutrition / v.24, no.3, 2011 , pp. 291-294 More about this Journal
Abstract
A lipase inhibitor from Desmodium oxyphyllum DC. was purified by methanol extraction, systematic solvent extraction, silica gel column chromatography, $C_{18}$ solid phase extraction chromatography and RP-HPLC. We obtained the purified lipase inhibitor with 182 ng($IC_{50}$) of lipase inhibitory activity for a 0.06% yield. Its molecular weight was estimated to be 655.37 Da from an instrumental analysis of MALDI-TOF-MS and it was identified copper-3,5-dibromo-2-hydroxybenzoic acid ($C_{14}H_8Br_4CuO_6$) by $^1H$, $^{13}C$ NMR analysis.
Keywords
lipase inhibitor; Desmodium oxyphyllum DC.; purification;
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Times Cited By KSCI : 1  (Citation Analysis)
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