Browse > Article
http://dx.doi.org/10.7318/KJFC/2021.36.1.130

Inhibitory Effects of 14 Plants from Mongolia and Myanmar on Lipid Accumulation in 3T3-L1 and HepG2 Cells  

Kim, SukJin (Department of Bio-Health Convergence Major, Duksung Women's University)
Kim, Gun-Hee (Department of Bio-Health Convergence Major, Duksung Women's University)
Publication Information
Journal of the Korean Society of Food Culture / v.36, no.1, 2021 , pp. 130-142 More about this Journal
Abstract
This study examined the antioxidative and lipid accumulation inhibitory effects of 14 plants from Mongolia and Myanmar on 3T3-L1 and HepG2 cells. The total phenolic and flavonoid contents (TPC and TFC) of 14 plant extracts were measured, and the antioxidative activities were analyzed using DPPH, ABTS, FRAP, and ORAC. After measuring the pancreatic lipase levels and performing the thiobarbituric acid assay, the degree of lipid accumulation was determined by lipid (Oil Red O) staining and triglyceride assay in 3T3-L1 and HepG2 cells. M. paniculate (259.43 mgGAE/g) and C. benghalensis (130.78 mgNAE/g) had the highest TPC and TFC, respectively, among the 14 plants. R. acicularis Lindl. had the highest antioxidant activity in DPPH. The ABTS, FRAP, and ORAC results showed that the antioxidant activity of 11 species was higher than that of the positive control. The pancreatic lipase inhibitory effect of C. angustifolium Scop. was reduced to 23.65% at 0.1 mg/mL, and the level of lipid peroxidation of C. abrorescens Lam. was 0.63 nmol/mg. Five selected plants inhibited the lipid accumulation and triglyceride content, respectively, in 3T3-L1 and HepG2 cells. These results provide scientific evidence for developing functional foods using 14 plants from Mongolia and Myanmar, which have antioxidant activities and lipid accumulation reduction effects.
Keywords
Mongolia; myanmar; antioxidant; lipid accumulation; 3T3-L1; hepG2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Gurnani N, Gupta M, Mehta D, Mehta BK. 2018. Chemical composition, total phenolic and flavonoid contents, and in vitro antimicrobial and antioxidant activities of crude extracts from red chilli seeds (Capsicum frutescens L.). J. Taibah Univ. Med. Sci., 10:462-470   DOI
2 GutiErrez-Grijalva EP, Ambriz-Pere DL, Leyva-Lopez N, Castillo-Lopez RI, Heiedia JB. 2016. Review: dietary phenolic compounds, health benefits and bioaccessibility. Arch. Latinoam. Nutr., 66(2):87-100
3 Jeon YS, Jo BS, Park HJ, Kang SA, Cho YJ. 2012. Screening of biological activity of Caragana sinica extracts. J. Korean Soc. Food Sci. Nutr., 41(9):1211-1219   DOI
4 Kang SK. 2012. Climatic and socio-ecological considerations on yellow dust and desertification in dryland regions of the northeast Asia. Korea J. Nat. Conserv., 6(1):1-8
5 Karadag A, Ozcelik B, Saner S. 2009. Review of methods to determine antioxidant capacities. Food Anal. Methods, 2(1):41-60   DOI
6 Kawano Y, Cohen DE. 2013. Mechanisms of hepatic triglyceride accumulation in non-alcoholic fatty liver disease. J. Gastroenterol., 48(4):434-441   DOI
7 Kim SJ, Kim GH. 2020. Plants from Ulleung island ameliorate lipid accumulation and oxidative stress in oleic acid-induced in HepG2 Cells. Korean Soc. Food Cult.,27(6):817-828
8 Kraus NA, Ehebauer F, Zapp B, Rudolphi B, Kraus BJ, Kraus D. 2016. Quantitative assessment of adipocyte differentiation in cell culture. Adipocyte, 5(4):351-358   DOI
9 Kumar P, Sharma S, Khanna M, Raj HG. 2003. Effect of Quercetin on lipid peroxidation and changes in lung morphology in experimental influenza virus infection. Int. J. Exp. Pathol, 84(3):127-133   DOI
10 Lewis WH. 1959. A monograph of the genus Rosa in North America. I. R. acicularis. Brittonia, 11:1-24   DOI
11 Li R, Jia Z, Trush MA. 2016. Defining ROS in biology and medicine. React Oxyg. Species (Apex), 1(1): 9-21
12 Lim SM, Goh YM, Kuan WB, Loh SP. 2014. Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes. Lipids Health Dis., 13(169):1-9
13 Liu TT, Liu XT, Chen QX, Shi Y. 2020. Lipase Inhibitors for Obesity: A Review. Biomed. Pharmacother., 128(110314):1-9
14 Liu W, Yin D, Li N, Hou X, Wang D, Li D, Liu J. 2016. Influence of environmental factors on the active substance production and antioxidant activity in Potentilla fruticosa L. and its quality assessment. Sci. Rep., 6(28591):1-18   DOI
15 Madrigal-Carballo S, Rodriguez G, Krueger CG, Dreher M, Reed JD. 2009. Pomegranate (Punica granatum) supplements: Authenticity. J. Funct. Foods, 1(3):324-329   DOI
16 Marjani A. 2015. A review on the role of triglyceride in metabolic syndrome. Asian J. Pharm. Clin. Res., 8(3):1-3
17 McMurray F, Patten DA, Harper ME. 2016. Reactive oxygen species and oxidative stress in obesity- recent findings and empirical approaches. Obesity, 24(11):2301-2310   DOI
18 Morales M, Giraldo SZ, Jaimes T, Delgado SR, Arbelaez AFA, Maldonado ME, Zamorano P, Rojano B. 2017. Mangiferin content, carotenoids, tannins and oxygen radical absorbance capacity (ORAC) values of six mango (Mangifera indica) cultivars from the Colombian Caribbean. J. Med. Plant Res., 11(7):144-152   DOI
19 Meghwal PR, Singh A, Kumar P, Morwal BR. 2014. Diversity, distribution and horticultural potential of Cordia myxa L.: a promising underutilized fruit species of arid and semi arid regions of India. Genet. Resour. Crop. Evol., 61:1633-1643   DOI
20 Melo PEF, Silva APM, Marques FP, Ribeiro PRV, Souza Filho MDSM, Brito ES, Lima JR, Azeredo HMC. 2019. Antioxidant films from mango kernel components. Food Hydrocoll., 95:487-495   DOI
21 Morita M, Ishida N, Uchiyama K, Yamaguchi K, Itoh Y, Shichiri M, Yoshida Y, Hagihara Y, Naito Y, Yoshikawa T, Niki E. 2012. Fatty liver induced by free radicals and lipid peroxidation. Free Radic. Res., 46(6):758-765   DOI
22 Niki E, Yoshida Y, Saito Y, Noguchi N. 2005. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochem. Biophys. Res. Commun., 338(1):668-76   DOI
23 Odontuya G. 2016. Anti-oxidative, acetylcholinesterase and pancreatic lipase inhibitory activities of compounds from Dasiphora fruticosa, Myricaria alopecuroides and Sedum hybridum. Mong. J. Chem., 17(43)42-49   DOI
24 Okuno Y, Fukuhara A, Hashimoto E, Kobayashi H, Kobayashi S, Otsuki M, Shimomura L. 2018. Oxidative stress inhibits healthy adipose expansion through suppression of SREBF-1-mediated lipogenic pathway. Diabetes, 67(6): 1113-1127.   DOI
25 Saha P, Talukdar AD, Nath R, Sarker SD, Nahar L, Sahu J, Choudhury MD. 2019. Role of natural phenolics in hepatoprotection: a mechanistic review and analysis of regulatory network of associated genes. Front. Pharmacol., 10(509):1-25   DOI
26 Ollanketo M, Peltoketo A, Hartonen K, Hiltunen R. Riekkola ML. 2002. Extraction of sage (Salvia officinalis L.) by pressurized hot water and conventional methods: antioxidant activity of the extracts. Eur. Food Res. Technol., 215:158-163   DOI
27 Park JY, Kim CS, Park KM, Chang PS. 2019. Inhibitory characteristics of flavonol-3-O-glycosides from Polygonum aviculare L. (common knotgrass) against porcine pancreatic lipase. Sci. Rep., 9(18080):1-10   DOI
28 Pavel S, Klejdus B, Kuban V. 2006. Determination of total content of phenolic compounds and their antioxidant activity in vegetables evaluation of spectrophotometric methods. J. Agr. Food Chem., 54(3):607-16   DOI
29 Poli G, Albano E, Dianzani MU. 1987. The role of lipid peroxidation in liver damage. Chem. Phys. Lipids, 45(204):117-42   DOI
30 Shah P, Modi HA. 2015. Comparative study of DPPH, ABTS and FRAP assays for determination of antioxidant activity. Int. j. res., 3(4):2321-9653
31 Sorokina M, Steinbeck C. 2020. Review on natural products databases: where to find data in 2020. J Cheminform, 12(20):1-51   DOI
32 Sternberg T, Tsolmon R, Middleton NM, Thomas DSG. 2011. Tracking desertification on the Mongolian steppe through NDVI and field-survey data. Int. J. Digit. Earth, 4(1):50-64   DOI
33 Tomczyk M., Paduch R., Wiater A., Pleszczynska M., Kandefer-Szerszen M., Szczodrak J. 2013. The influence of aqueous extracts of selected Potentilla species on normal human colon cells. Acta. Pol. Pharm., 70(3):523-531.
34 Wadje SD, Wankhede BG, Mali MR. 2019. Phytochemical and in vitro antioxidant activity of Careya arborea Roxb. leaves successive extracts. J. drug. deliv. Ther., 9(2-S):53-56
35 Truong D.H., Nguyen D.H., Ta N.T.A., Bui A.V., Do T.H., Nguyen, H.C. 2019. Evaluation of the Use of different solvents for phytochemical constituents, antioxidants, and in vitro anti-inflammatory activities of Severinia buxifolia. J. Food. Qual., 2019:1-9.
36 Um ES, Kim YS. 2016. Effect of Samhwangsasim-tang and Daehwanghwangryunsasim-tang on palmitate-induced lipogenesis in HepG2 cells. Korean J. Orient. Med. Prescr., 37(1): 62-76
37 Urgamal M, Baasansuren E, Tovuudorj ME, Shijirbaatar O, Chinbaatar Z, Lkhagvadorj K, Kwon OS. 2018. Medicinal plant diversity in the southern and eastern Gobi Desert region, Mongolia. J. Eco. Env., 42(4):1-13   DOI
38 Wahyono A, Dewi AC, Yudiastuti SON, Jamilah S, Kang WW. 2020. Antioxidant activity and total phenolic contents of bread enriched with pumpkin flour. IOP Conf. Ser. Earth Environ. Sci., 411:012049   DOI
39 Walker JM. 1994. The bicinchoninic acid (BCA) assay for protein quantitation. Methods Mol. Biol., 32:5-8
40 Wang H, Eckel RH. 2009. Lipoprotein lipase: from gene to obesity. Am. J. Physiol. Endocrinol. Metab., 297: E271-E288   DOI
41 Wang T, Takikawa Y, Tabuchi T, Satoh T, Kosaka K, Suzuki K. 2012. Carnosic acid (CA) prevents lipid accumulation in hepatocytes through the EGFR/MAPK pathway. J. Gastroenterol., 47(7):805-813   DOI
42 Werkhoven CHE, Salisbury PJ. 1966. Germination and survival of Colorado spruce, Scots pine, Caragana and Siberian elm at four salinity and two moisture levels. Can. J. Plant Sci., 46(1):1-7   DOI
43 Balachander GJ, Subramanianb S, langoK. 2018. Rosmarinic acid attenuates hepatic steatosis by modulating ER stress and autophagy in oleic acid-induced HepG2 cells. RSC. Adv., 8:26656-26663   DOI
44 Williamson G, Kay CD, Crozier A. 2018. The bioavailability, transport, and bioactivity of dietary flavonoids: a review from a historical perspective. COMPR. REV. FOOD SCI. F., 17(4):1-59   DOI
45 Yang J, Fernandez-Galilea M, Martinez-Fernandez L, Gonzalez-Muniesa P, Perez-Chavez A, Martinez JA, Moreno-Aliaga MJ. 2019. Oxidative stress and non-alcoholic fatty liver disease: effects of omega-3 fatty acid supplementation. Nutrients, 11(4): 872   DOI
46 Young I, Woodside J. 2001. Antioxidants in health and disease. J. Clin. Pathol., 54(3):176-186   DOI
47 Agnieszka G, Mariola D, Anna P, Piotr K, Natalia W, Aneta S, Marcin O, Bogna O, Zdzislaw L, Aurelia P, Magdalena M, Lukasz MP, Karolina W. 2018. Qualitative and quantitative analyses of bioactive compounds from ex vitro Chamaenerion angustifolium (L.) (Epilobium augustifolium) herb in different harvest times. IND. CROP. PROD., 123:208-220   DOI
48 Ahn EM, Kang HJ, Kim Y, Choe YS, Kang MS. 2015. Effects of ethanol extracts from commonly consumed vegetables in Korea on differentiation and secretion of MCP-1 and adiponectin in 3T3-L1 adipocytes and lipid accumulation in HepG2 hepatocytes. J. East Asian Soc. Dietary Life 25(1): 99-110   DOI
49 Amalina N, Bakar MFA, Bakar FIA, Rahim AC, Murdin N. 2019. Underutilized Mangifera species (Mangifera caesia, Mangifera quadrifida and Mangifera odorata) from Borneo: a potential source of antioxidant. J. Eng. Appl., 14(4):1169-1177   DOI
50 Apovian CM. 2016. Obesity: definition, comorbidities, causes, and burden. Am. J. Manag. Care, 22(7):s176-s185
51 Benedict M, Zhang X. 2017. Non-alcoholic fatty liver disease: an expanded review. World J. Hepatol., 9(16):715-732   DOI
52 Biernasiuk A, Wozniak M, Bogucka-Kocka A. 2015. Determination of free and bounded phenolic acids in the rhizomes and herb of Sanguisorba officinalis L. Curr. Issues Pharm. Med.. Sci., 28(4):254-256   DOI
53 Blokhina O, Virolainen E, Fagerstedt KV. 2003. Antioxidants, oxidative damage and oxygen deprivation stress: a review. Ann. Bot., 91(2):179-19   DOI
54 Boccellino M, D'Angelo S. 2020. Anti-obesity effects of polyphenol intake:current status and future possibilities. Int. J. Mol. Sci., 21:5642   DOI
55 D'Angiolillo F, Mammano MM, Fascella G. 2018. Pigments, polyphenols and antioxidant activity of leaf extracts from four wild rose species grown in Sicil. Not. Bot. Horti. Agrobo., 46(2): 402-409   DOI
56 Breitenbach M, Eckl P. 2015. Introduction to oxidative stress in biomedical and biological research. Biomolecules, 5(2): 1169-1177   DOI
57 Buchholz T, Melzig MF. 2015. Polyphenolic compounds as pancreatic lipase inhibitors. Planta. Med., 81(10):771-783   DOI
58 Choi SE, Lee WK, Yu HN, Kang HD, Kim YS. 2014. Analysis of Relationship between Land Cover Change and Vegetation Temperature Condition Index in Central Dry Zone of Myanmar. Korean Assoc. Geogr. Inf. Stud., 17(2):82-94
59 DeFilipps RA, Krupnick GA. 2018. The medicinal plants of Myanmar. PhytoKeys. PhytoKeys, 102:1-341   DOI
60 Dissanayake1 DP, Abeytunga1 DTU, Vasudewa1 NS, Ratnasooriya WD. 2009. Inhibition of lipid peroxidation by extracts of Pleurotus ostreatus. Pharmacogn. Mag., 5(19):266-271
61 Abenavoli L, Milic N, Luzza F, Boccuto L, Lorenzo A. 2017. Polyphenols Treatment in Patients with Nonalcoholic Fatty Liver Disease. J Transl. Int. Med., 5(3):144-147   DOI