The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Purification and Characterization of Lipase Inhibitor from Desmodium oxyphyllum DC.

도둑놈의 갈고리의 지질분해효소 저해물질의 정제 및 특성

  • Lee, Jong-Kug (Chungnam Agricultural Research and Extension Services) ;
  • Kang, Min-Gu (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Baek, Seung-Ye (Dept. of Life Science and Genetic Engineering, Paichai University) ;
  • Ahn, Young-Geun (Faculty of Food Nutrition, Chungcheong University) ;
  • Lee, Jong-Soo (Dept. of Life Science and Genetic Engineering, Paichai University)
  • 이종국 (충남 농업기술원) ;
  • 강민구 (배재대학교 생명유전공학과) ;
  • 백승예 (배재대학교 생명유전공학과) ;
  • 안용근 (충청대학 식품영양학부) ;
  • 이종수 (배재대학교 생명유전공학과)
  • Received : 2011.04.18
  • Accepted : 2011.07.15
  • Published : 2011.09.30
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Abstract

A lipase inhibitor from Desmodium oxyphyllum DC. was purified by methanol extraction, systematic solvent extraction, silica gel column chromatography, $C_{18}$ solid phase extraction chromatography and RP-HPLC. We obtained the purified lipase inhibitor with 182 ng($IC_{50}$) of lipase inhibitory activity for a 0.06% yield. Its molecular weight was estimated to be 655.37 Da from an instrumental analysis of MALDI-TOF-MS and it was identified copper-3,5-dibromo-2-hydroxybenzoic acid ($C_{14}H_8Br_4CuO_6$) by $^1H$, $^{13}C$ NMR analysis.

Keywords

References

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