• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1314-1319
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• 2009

In previous study, we have shown that continentalic acid (CA) isolated from Aralia continentalis induced the growth inhibition and apoptosis in HepG2 cells. In this study, we examine the effects of CA from A. continentalis on growth inhibition and apoptosis induction in human leukemia HL-60 and mouse fibroblast NIH 3T3 cell lines. The results demonstrated that CA decreased cell growth of leukemia HL-60 cells but not human HaCaT keratinocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of mouse fibroblast cell lines exposed to CA showed that apoptotic cells increased in a time- and dose-dependent manner. Treatment with CA decreased the number of normal cells and increased the number of early apoptotic and late apoptotic cells in a dose-dependent manner. The induction of apoptosis in mouse cell lines by CA was mediated through the activation of caspase-3, Bak, and Bax and the down-regulation of Bcl-2. Our results suggest that CA efficiently induces apoptosis in human leukemia cells.

• Journal of Plant Biotechnology
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• v.47 no.1
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• pp.40-45
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• 2020

An efficient protocol for multiple shoot induction and plant regeneration from axillary bud culture of Magnolia 'Vulcan' was developed in the present study. Primary shoots were obtained from axillary bud explants cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BA). To induce multiple shoots effectively, primary shoot tips were cultured on MS medium supplemented with different concentrations of BA and zeatin at 0, 0.2, 0.5, and 1.0 mg/L. Of these treatments, the MS medium with 0.5 mg/L BA resulted in the highest number of shoots per explant with an average value of 5.9, and it produced the greatest shoot height at 4.8 cm after 12 weeks of culturing. In the rooting of in vitro produced shoots, the greatest percentage of explants forming roots (91.3%), number of roots per explant (9.7), and root length (2.8 cm) were obtained in half-strength MS medium supplemented with 6.0 mg/L indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized and hardened off inside the culture room with 87.5% survival rate. Plants were transferred to a greenhouse with a 97.2% survival rate. The highly efficient shoot multiplication and plant regeneration system reported herein can be used for large-scale clonal propagation of valuable Magnolia species or cultivars.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.18 no.8
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• pp.772-778
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• 2005

In this paper, we designed the cylindrical type light source that had a electromagnetic principle of inductively coupled plasma, and measured its electrical-optical properties. Using the principle of transformer, electrically equivalent circuit of cylindrical type light source was analyzed. According to the parameters of electromagnetic induction which were diameter of coil with $0.3\~1.2\;mm{\Phi}$, number of turns with $4\~12$ turns, distance with $40\~120$ mm and RF power with $10\~150$ W, the electrical and optical properties were measured. When diameter of coil was $0.3\;mm{\Phi}$, number of turns was 8 turns and distance was 40 mm, the highest brightness of 29,730 $cd/m^2$ was shown with RF power 150 W. The relationship between electromagnetic induction and plasma discharges was shown by mode transition from E-mode to H-mode.

• Journal of Embryo Transfer
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• v.1 no.1
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• pp.69-75
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• 1986

These studies were carried out to investigate the induction of superovulation, the synchroniration and the effect of the number of embryos transferred, the developmentai stage of embryos and the donor-recipient synchrony on pregnancy rate in cattle. The results obtained in these studies were as follows: 1. The number of oorpus leuteum(CL) and the embryos reoovered were higher in FSH treated animals than in PMSG treated (9.4 vs 8.1 and 6.1 vs 4.5) and showed the same trend in recovery rate (64.9% vs 55.6%). 2. Two shots of cioprostenol at 11 days showed significantly high no. of animals in estrus in order of crossbred, Holstein and Korean native cattle. No significant differencies were noted in the seoond shots groups. And the interval to estrus and response were shorter and better in second shot group than that in first shot group. 3. The pregnancy rate when additional one embryo was transferred after Al at estrus (52.8%) was higher than the group transferred one (32.8%) or two (36.9%) embryos. 4. The pregnancy rate of embryo in morula stage was better than that in blasto cyst stage (39.3% vs 32.5%). 5. The pregnancy rate in the recipients exhibited estrus later than the donor (66.7%) was the higher than those exhibited estrus (40.3%) or exhibited estrus before the donor (37.5%).

• Plant Biotechnology Reports
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• v.4 no.2
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• pp.173-178
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• 2010

An efficient protocol for shoot regeneration was developed for sesame (Sesamum indicum L.) internodes using the transverse thin cell layer (tTCL) culture method. The frequency of shoot regeneration and the number of adventitious buds produced from regenerated shoots depend significantly on explant age, thickness of the tTCL sections, and the phytohormones supplemented to the culture medium. A combination of 6-benzyladenine (2.0 $mg\;l^{-1}$) and a-naphthaleneacetic acid (0.5 $mg\;l^{-1}$) was found to be the best phytohormone combination for shoot bud induction, with the maximum number of shoots obtained when the tTCL sections were 0.5-1.0 mm thick and derived from 4- to 6-week-old seedlings of sesame. Well-developed shoots were rooted on MS medium without phytohormones, and 80% of the regenerated plantlets were successfully established in soil.

• Mycobiology
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• v.36 no.1
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• pp.40-44
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• 2008

Various bacteria were isolated from the casing layer soil of the culture bed of P. ostreatus and their role in fruiting body induction of the edible mushroom, P. ostreatus, was investigated. Analysis of the bacterial community isolated from the casing layer soil revealed that the composition of genera and number of cultivable bacteria were different for each sterilizing treatment. Bordetella was predominant in the bulk soil whereas Flavobacterium was predominant after sterilization of the casing layer soil. Fluorescent Pseudomonas was predominant in the non-sterilized casing layer soil. Total number of the bacterial genera in the casing layer soil was higher than that in the bulk soil. In particular, an increase in the fluorescent Pseudomonas population was observed in the non-sterilized casing layer accompanied by induction of fruiting body and enhanced mushroom production yield. The results suggested that specific bacterial populations in the casing layer play an important role in the formation of primodia and the development of basidiome in P. ostreatus.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.12a
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• pp.81-86
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• 2006

In this paper, we designed the cylindrical type light source that had a electromagnetic principle of inductively coupled plasma, and measured its electrical-optical properties. Using the principle of transformer, electrically equivalent circuit of cylindrical type light source was analyzed. According to the parameters of electromagnetic induction which were diameter of coil with 0.3~1.2 mm, number of turns with 4~12 turns, distance with 40~120 mm and RF power with 10~150 W, the electrical and optical properties were measured. When diameter of coil was 0.3 mm, number of turns was 8 turns and distance was 40 mm, the highest brightness of 29,730 $cd/m^2$ was shown with RF power 150 W. The relationship between electromagnetic induction and plasma discharges was shown by mode transition from E-mode to H-mode.

• Journal of Venture Innovation
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• v.1 no.1
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• pp.197-210
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• 2018

The purpose of this study is to analyze the relationship between the induction servicescape and the user satisfaction and repurchase intention about the restaurant tables embedded with the most advanced eco - friendly induction range. The main results of this study are as follows. The demographic characteristics of the respondents were 104 male respondents and 106 female respondents, with male - female ratio almost similar. The respondents' positions and responsibilities consisted of 68 restaurant representatives and 142 restaurant managers or persons in charge. The number of restaurants embedded with the induction ranges staffing 5 or more employees was 62.4%, which clearly showed that they were larger than the average restaurants(2.8 employees). The result of hypothesis testing by regression analysis showed that the restaurant's eco - friendly functional induction servicescape had a significant effect on user satisfaction, and ② the eco - friendly functional induction servicescape of the restaurant had a significant influence on repurchase intention. ③ The operation of the restaurant with the induction of the eco - friendly function of the restaurant. The satisfaction of the manager has a significant influence on the repurchase intention. Therefor, the hypothesis suggested in this study was adopted.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.3
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• pp.476-482
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• 2019

Regulatory protocols such as the Convention on Climate Change and the regulation of greenhouse gas emissions act as catalysts for the development of high-efficiency energy equipment and the efficient use of energy. Among the fields where energy consumption is high, the electric heating equipment is not efficient. The electric boiler mainly uses a method of circulating water by contacting the heater. When the existing electric boiler is used, the water minerals are contacted with the high-temperature heater to be carbonized and adsorbed, thereby promoting the corrosion of the heater and lowering the efficiency of the heater. For this reason, an electric induction boiler, which has high energy efficiency and is applied to an induction heating system that can uniformly heat the object to be heated rather than conduction or convection heating, is in the limelight. This method induces a boiler pipe And it is recognized as an alternative that can solve the problem that occurs when heating is performed by direct heating. Despite the fact that research on induction heating has been conducted for a relatively long period of time, there have been few studies on the electrothermal technology using induction heating. Therefore, in this paper, to improve the heat efficiency of electric induction boiler, the influence of the cross sectional area, number of windings and winding layers is analyzed by finite element method through parametric study method. The method of finding the design point which maximizes the total loss is proposed by the alternating winding design method which can maximize the heat generation by analyzing copper and iron losses.

• Fisheries and Aquatic Sciences
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• v.4 no.4
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• pp.208-218
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• 2001

We have investigated the gonad index (GI), gonadal development, reproductive cycle, first sexual maturity, sex ratio, the number of spawned eggs and spawning frequency of the Manila clam, Ruditapes philippinarum. Samples were collected from the intertidal zone of Komso Bay, Korea from January to December in 1999. Monthly changes in the gonad index (GI) and condition index showed a similar pattern in the reproductive cycle. The spawning period was once a year between early June and early October, there was a spawning peak between July and August when seawater temperature was over $20^{\circ}C$. The reproductive cycle of this species can be categorized into five successive stages; early active (February to March), late active (April to May), ripe (April to August), partially spawned (June to October), and spent/inactive stage (August to March). Percentages of first sexual maturity of female and male clams of l5.1-20.0mm in shell length were $56.3\%$ and $60.0\%$, respectively, and $100\%$ for the clams >25. mm. The sex ratio of individuals >15.1 mm in shell length was about 1:1 $(\chi^2= 0.02,\;p>0.05)$. Number of the eggs released from each clam by the induction increased as the size of clam in terms of shell length increased. Mean number of the eggs from the second induction of the spawning was $75.35-84.30\%$ $(average\;79.81\%)$ of the number of the eggs released in the first spawning. Our data indicated that R. philippinarum in Komso Bay has one major spawning peak with over two minor spawning, and the interval of each spawning was estimated to be approximately 15-17 (average 16.5) days.