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Effects of Continentalic from Aralia Continentalis on Growth Inhibition and Apoptosis Induction in Human Leukemia HL-60 Cells  

Kim, Sun-Young (Department of Physiology, Center for Healthcare Technology Development, Chonbuk National University Medical School)
Jeong, Seung-Il (Jeonju Biomaterials Institute)
Kim, Sung-Zoo (Department of Physiology, Center for Healthcare Technology Development, Chonbuk National University Medical School)
Shim, Jae-Suk (Imsilsangyak Farming Association)
Jang, Seon-Il (Jeonju University Atopy & Health Research Institute)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.23, no.6, 2009 , pp. 1314-1319 More about this Journal
Abstract
In previous study, we have shown that continentalic acid (CA) isolated from Aralia continentalis induced the growth inhibition and apoptosis in HepG2 cells. In this study, we examine the effects of CA from A. continentalis on growth inhibition and apoptosis induction in human leukemia HL-60 and mouse fibroblast NIH 3T3 cell lines. The results demonstrated that CA decreased cell growth of leukemia HL-60 cells but not human HaCaT keratinocytes, assessed with the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] and LDH (lactate dehydrogenase) assay. Flow cytometric analysis of mouse fibroblast cell lines exposed to CA showed that apoptotic cells increased in a time- and dose-dependent manner. Treatment with CA decreased the number of normal cells and increased the number of early apoptotic and late apoptotic cells in a dose-dependent manner. The induction of apoptosis in mouse cell lines by CA was mediated through the activation of caspase-3, Bak, and Bax and the down-regulation of Bcl-2. Our results suggest that CA efficiently induces apoptosis in human leukemia cells.
Keywords
continentalic acid; Aralia continentalis; apoptosis; mouse fibroblast cell lines;
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