• Food Science and Biotechnology
• /
• 제16권4호
• /
• pp.655-658
• /
• 2007

Bacillus polyfermenticus SCD was transformed by the recombinant shuttle vector for Bacillus and Escherichia coli containing 3 antibiotic resistant genes and an ${\alpha}$-amylase gene from Bifidobacterium adolescentis Int57. The ${\alpha}$-amylase gene fused to a secretion sequences was expressed under the control of the promoter of amylase gene from B. subtilis var. natto. The recombinant plasmid was maintained stably in the transformants producing the ${\alpha}$-amylase. The enzyme was secreted to outside of the cell and showed the similar enzyme activity as that of Bacillus subtilis BD170 under the same conditions of pH and growth temperature. Because of the relatively easy transformation and the secretion of the enzyme, the transformants of B. polyfermenticus SCD may give a new strategy in the production of foreign genes.

• 한국농공학회논문집
• /
• 제65권2호
• /
• pp.13-20
• /
• 2023

In the past, the Ministry of Agriculture, Food, and Rural Affairs (MAFRA) conducted research into and production of agricultural statistics. As a result of organizational changes, these responsibilities were transferred to Statistics Korea in 1998 and 2008. So, the MAFRA managed data without a formal system for managing data and statistical information. The demand for a foundation to improve the usefulness of agri-food statistics has recently increased due to the necessity for basic data representing the realities of rural and agricultural areas. This is especially true for field-oriented agricultural administration. This study aims to propose a strategy for the systematically integrated administration of dispersed data and statistical information. It served as the foundation for the integrated management of distributed administrative and statistical data.

• Journal of Ginseng Research
• /
• 제29권3호
• /
• pp.152-158
• /
• 2005

몇 천년동안 고려인삼은 동양국가에서 최고의 약재로 사용되어 왔다. 한국은 인삼종주국이며 이러한 이유로 한국의 특용작물인 인삼은 삼국시대부터 세계로 수출되어 왔다. 그러나 최근에 중국과 캐나다의 거대한 인삼 생산량 때문에 고려인삼 제품의 국제 시장 점유율이 급격히 떨어지고 있다. 게다가 미국은 공격적인 시장전략을 가지고 국제 인삼시장을 이끌고있다. 그러므로 인삼 종주국으로서의 한국은 국제 인삼시장의 상황과 분위기를 이해해야만 한다. 이 연구의 목적은 세계 시장에서 주요 경쟁자들의 인삼 소비실태와 소비인지를 구명하는 것이다. 연구의 발견은 소비자 행동과 주요 국가의 인식차이를 보여준다. 결과적으로 이 연구는 다방면의 인삼분야에 사용하는데 응용될 것이라고 기대한다.

• 한국토양비료학회지
• /
• 제42권Spc호
• /
• pp.29-32
• /
• 2009

전통적으로, 한국의 벼생산 시스템은 곡물생산이라는 주목적 이외에, 지하수 함양, 장마기의 담수기능에 의한 홍수 방지 등 논농사가 가지는 다원적 기능이 주요한 특징으로 자리잡고 있다. 이러한 의미에서, 앞으로 다가올 기후변화에 대응한 새로운 작물생산 기술의 개발은 벼 생산 시스템의 다원적 기능과 IT, BT, NT를 융합한 체계로 나아가야 할 필요가 있다. 이와 같은 요구에 부응하기 위해서는 현재의 농업생산체계가 다음과 같은 새로운 파라다임으로 전환해야 할 것이다. 우선, 식량과 에너지를 생산하는 터전인 농경지의 중요성과 가치를 재인식하고 유지 보전 시켜야 할 것이다. 둘째, 농업생산 구조가 에너지 수지를 고려한 저탄소 녹색 농업체계로 전환하여야 할 것이다. 마지막으로 논 농업이 가진 공익적 기능을 새롭게 인식하고 이를 극대화시키는 자세 전환이 필요할 것이다.

• Nutrition Research and Practice
• /
• 제10권2호
• /
• pp.131-138
• /
• 2016

BACKGROUND/OBJECTIVES: Citrus and its peels have been used in Asian folk medicine due to abundant flavonoids and usage of citrus peels, which are byproducts from juice and/or jam processing, may be a good strategy. Therefore, the aim of this study was to examine antioxidant and anti-inflammatory effects of bioconversion of Jeju Hallabong tangor (Citrus kiyomi ${\times}$ ponkan; CKP) peels with cytolase (CKP-C) in RAW 264.7 cells. MATERIALS/METHODS: Glycosides of CKP were converted into aglycosides with cytolase treatment. RAW 264.7 cells were pre-treated with 0, 100, or $200{\mu}g/ml$ of citrus peel extracts for 4 h, followed by stimulation with $1{\mu}g/ml$ lipopolysaccharide (LPS) for 8 h. Cell viability, DPPH radical scavenging activity, nitric oxide (NO), and prostagladin $E_2$ ($PGE_2$) production were examined. Real time-PCR and western immunoblotting assay were performed for detection of mRNA and/or protein expression of pro-inflammatory mediators and cytokines, respectively. RESULTS: HPLC analysis showed that treatment of CKP with cytolase resulted in decreased flavanone rutinoside forms (narirutin and hesperidin) and increased flavanone aglycoside forms (naringenin and hesperetin). DPPH scavenging activities were observed in a dose-dependent manner for all of the citrus peel extracts and CKP-C was more potent than intact CKP. All of the citrus peel extracts decreased NO production by inducible nitric oxide synthase (iNOS) activity and $PGE_2$ production by COX-2. Higher dose of CKP and all CKP-C groups significantly decreased mRNA and protein expression of LPS-stimulated iNOS. Only $200{\mu}g/ml$ of CKP-C markedly decreased mRNA and protein expression of cyclooxygenase-2 in LPS-stimulated RAW 264.7 cells. Both 100 and $200{\mu}g/ml$ of CKP-C notably inhibited mRNA levels of $interleukin-1{\beta}$ ($IL-1{\beta}$) and IL-6, whereas $200{\mu}g/ml$ CKP-C significantly inhibited mRNA levels of $TNF-{\alpha}$. CONCLUSIONS: This result suggests that bioconversion of citrus peels with cytolase may enrich aglycoside flavanones of citrus peels and provide more potent functional food materials for prevention of chronic diseases attributable to oxidation and inflammation by increasing radical scavenging activity and suppressing pro-inflammatory mediators and cytokines.

• 한국경제지리학회지
• /
• 제25권3호
• /
• pp.397-419
• /
• 2022

글로벌 식량 위기의 해결책이자, 4차 산업혁명 시대의 새로운 성장동력으로서 스마트농업이 등장하였다. 본 연구는 해외 주요국의 스마트농업 동향을 통해 각 국가의 농업 지식과 경험이 특정 농업 환경에서 어떻게 발현되는지 살펴봄으로써 스마트농업의 공간성을 밝히는데 목적이 있다. 이를 위해 스마트농업 기술 수준에 따라 중국, 일본, 네덜란드, 미국을 대표적인 사례로 선정하여 각 국가 스마트농업 정책의 방향성, 거버넌스, 주력 분야와 기술을 살펴보았다. 그 결과 지역의 경제·사회·문화 및 생태적 맥락으로부터 탈맥락화 시키는 방향으로 작동하는 표준화 전략과 지리적 특성을 반영하여 각 국가별 스마트농업 모델을 구축하고자 하는 국지화 전략이 동시에 작동하며 각 국가의 정책과 기술을 형성하는 것으로 판단된다. 본 연구는 국가적 스케일에서 스마트 농업 정책과 현황을 살펴보는 시도로써 농업기술 지리 및 농업지식생산의 지리를 탐색하기 위한 연구의 토대로 활용한다.

• Journal of Microbiology and Biotechnology
• /
• 제33권2호
• /
• pp.260-267
• /
• 2023

In this study, we sought to improve lutein and zeaxanthin production in Mychonastes sp. 247 and investigated the effect of environmental factors on lutein and zeaxanthin productivity in Mychonastes sp. The basic medium selection and N:P ratio were adjusted to maximize cell growth in one-stage culture, and lutein and zeaxanthin production conditions were optimized using a central composite design for two-stage culture. The maximum lutein production was observed at a light intensity of 60 μE/m²/s and salinity of 0.49%, and the maximum zeaxanthin production was observed at a light intensity of 532 μE/m²/s and salinity of 0.78%. Lutein and zeaxanthin production in the optimized medium increased by up to 2 and 2.6 folds, respectively, compared to that in the basic medium. Based on these results, we concluded that the optimal conditions for lutein and zeaxanthin production are different and that optimization of light intensity and culture salinity conditions may help increase carotenoid production. This study presents a useful and potential strategy for optimizing microalgal culture conditions to improve the productivity of lutein and zeaxanthin, which has applications in the functional food field.

• Journal of Microbiology and Biotechnology
• /
• 제28권6호
• /
• pp.902-908
• /
• 2018

Optimization of the culture medium to maximize menaquinone-7 (MK-7) production by Bacillus subtilis strain KCTC 12392BP in static culture was carried out using statistical experimental methods, including one factor at a time, fractional factorial design, and response surface methodology (RSM). Maltose (carbon source), tryptone (nitrogen source), and glycerol (activator) were identified as the key medium components for MK-7 synthesis by the fractional factorial design, and were selected for statistical optimization by RSM. The statistical analysis indicated that, in the range that was studied, maltose, tryptone, and glycerol were all critical factors having profound effects on the production of MK-7, with their coefficients for linear and quadratic all significant at the p < 0.05 level. The established model was efficient and feasible, with a determination coefficient ($R^2$) of 0.9419. The predicted concentrations of maltose, tryptone, and glycerol in the optimal medium were determined as 36.78, 62.76, and 58.90 g/l, respectively. In this optimized medium, the maximum yield of MK-7 reached a remarkably high level of $71.95{\pm}1.00{\mu}g/ml$ after 9 days of static fermentation, which further verified the practicability of this optimized strategy.

• 한국수산과학회지
• /
• 제48권2호
• /
• pp.151-157
• /
• 2015

This study was conducted to evaluate the effect of lactic acid bacterial fermentation on the antioxidant and anti-inflammatory activity of an edible brown alga, Eisenia bicyclis. Lactic acid bacteria were inoculated into and cultivated in E. bicyclis water extract. The antioxidant activity of the extract was assayed before and following fermentation. Antioxidant activity was determined by assaying the levels of radical scavenging activity against 2,2'-diphenyl-1-picrylhydrazyl (DPPH), hydroxyl radical and alkyl radical. The lactic acid bacterial fermentation of E. bicyclis extract resulted in enhanced antioxidant activity. The greatest enhancement of antioxidant activity was seen in the DPPH radical scavenging assay, in which E. bicyclis extract was fermented by Pediococcus pentosaceus MBP-34 strain for 12 h. This fermented extract also exhibited higher inhibitory activity (96.66%) on nitric oxide production compared with other lactic acid bacterial fermented extracts or raw extract (189.60%). In conclusion, fermentation by bacterial strain is an attractive strategy for developing value-added food ingredients.

• Food Science and Biotechnology
• /
• 제17권5호
• /
• pp.990-995
• /
• 2008

A thermostable $\alpha$-L-arabinofuranosidases ($\alpha$-L-AFase) is an industrially important enzyme for recovery of L-arabinose from hemicellulose. The recombinant $\alpha$-L-AFase from Thermotoga maritima was expressed in Escherichia coli by using a constitutive pHCE or an inducible pRSET vectors. In batch fermentation, the constitutive expression system resulted in slightly faster growth rate (0.78 vs. 0.74/hr) but lower enzyme activity (2,553 vs. 3,723 units/L) than those of the induction system. When fed-batch fermentation was performed, biomass and enzyme activity reached the highest levels of 36 g/L and 9,152 units/L, respectively. The fed batch cultures performed superior results than batch culture in terms of biomass yield (4.62-5.42 folds) and enzyme synthesis (3.39-4.00 folds). In addition, the fed-batch induction strategy at high cell density resulted in the best productivity in cell growth as well as enzyme activity rather than the induction method at low cell density or the constitutive expression.