Food Science and Biotechnology

Korean Society of Food Science and Technology (한국식품과학회)
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Heterologous Expression of ${\alpha}$-Amylase Gene of Bifidobacterium adolescentis Int57 in Bacillus polyfermenticus SCD

  • Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University) ;
  • Kim, Il-Gi (Department of Biomedical Science, Hallym University) ;
  • Lee, Jin-Hyoung (Department of Biomedical Science, Hallym University) ;
  • Lee, Jang-Hyun (Division of Animal Life Science, Konkuk University) ;
  • Park, Kyu-Yong (Department of Food Science and Biotechnology, Kyungnam University) ;
  • Ji, Geun-Eog (Department of Food Science and Nutrition, Seoul National University) ;
  • Jin, Tae-Eun (Department of Biomedical Science, Hallym University) ;
  • Rhim, Seong-Lyul (Department of Biomedical Science, Hallym University)
  • Published : 2007.08.31
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Abstract

Bacillus polyfermenticus SCD was transformed by the recombinant shuttle vector for Bacillus and Escherichia coli containing 3 antibiotic resistant genes and an ${\alpha}$-amylase gene from Bifidobacterium adolescentis Int57. The ${\alpha}$-amylase gene fused to a secretion sequences was expressed under the control of the promoter of amylase gene from B. subtilis var. natto. The recombinant plasmid was maintained stably in the transformants producing the ${\alpha}$-amylase. The enzyme was secreted to outside of the cell and showed the similar enzyme activity as that of Bacillus subtilis BD170 under the same conditions of pH and growth temperature. Because of the relatively easy transformation and the secretion of the enzyme, the transformants of B. polyfermenticus SCD may give a new strategy in the production of foreign genes.

Keywords

References

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