Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Improvement of Lutein and Zeaxanthin Production in Mychonastes sp. 247 by Optimizing Light Intensity and Culture Salinity Conditions

  • Seong-Joo Hong (Department of Biological Engineering, Inha University) ;
  • Kyung June Yim (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Young-Jin Ryu (Department of Biological Engineering, Inha University) ;
  • Choul-Gyun Lee (Department of Biological Engineering, Inha University) ;
  • Hyun-Jin Jang (Laboratory of Chemical Biology and Genomics, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ji Young Jung (Microbial Research Department, Nakdonggang National Institute of Biological Resources) ;
  • Z-Hun Kim (Microbial Research Department, Nakdonggang National Institute of Biological Resources)
  • Received : 2022.11.04
  • Accepted : 2022.11.23
  • Published : 2023.02.28
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Abstract

In this study, we sought to improve lutein and zeaxanthin production in Mychonastes sp. 247 and investigated the effect of environmental factors on lutein and zeaxanthin productivity in Mychonastes sp. The basic medium selection and N:P ratio were adjusted to maximize cell growth in one-stage culture, and lutein and zeaxanthin production conditions were optimized using a central composite design for two-stage culture. The maximum lutein production was observed at a light intensity of 60 μE/m2/s and salinity of 0.49%, and the maximum zeaxanthin production was observed at a light intensity of 532 μE/m2/s and salinity of 0.78%. Lutein and zeaxanthin production in the optimized medium increased by up to 2 and 2.6 folds, respectively, compared to that in the basic medium. Based on these results, we concluded that the optimal conditions for lutein and zeaxanthin production are different and that optimization of light intensity and culture salinity conditions may help increase carotenoid production. This study presents a useful and potential strategy for optimizing microalgal culture conditions to improve the productivity of lutein and zeaxanthin, which has applications in the functional food field.

Keywords

Acknowledgement

This study was supported by a grant (Project No. NNIBR202303101) from the Nakdonggang National Institute of Biological Resources funded by the Ministry of Environment of the Korean government. This work was also supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF- 2022R1I1A1A01052953).

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