• 제목/요약/키워드: ecological trait

검색결과 33건 처리시간 0.021초

ISSR 마커를 이용한 달래와 산달래의 분류 (Classification of Allium monanthum and A. grai by ISSR Markers)

  • 이샛별;김창길;오중열;김경민
    • 원예과학기술지
    • /
    • 제29권6호
    • /
    • pp.600-609
    • /
    • 2011
  • Allium속에 포함된 6종의 122점을 수집하고, 이 종들의 유전적 관계는 ISSR 마커를 이용하여 확인하였다. 형태적 분석은 6개의 양적 형질을 측정하고 1개의 질적 형질은 수치화하였다. SSR 분석은 17개의 primer를 사용하여 총 370개의 다형성 밴드를 얻었다. 형태적 특성 분석은 유전적 거리로 구분할 경우 3개의 그룹으로 분류되었으나, 부분적으로 몇 몇 종들은 분류에 어려움이 있었다. ISSR 결과를 바탕으로 Allium 속의 군집분석은 5개의 그룹으로 분리되었다. 형태적 분석과 SSR분석 간의 상관 관계는 유의성이 매우 낮았다(r = 0.036). 따라서 본 연구에서 개발한 ISSR 마커는 달래와 산달래의 분류와 교배 육종에 유용하게 이용될 수 있을 것이다.

Plant Growth-Promoting Trait of Rhizobacteria Isolated from Soil Contaminated with Petroleum and Heavy Metals

  • Koo, So-Yeon;Hong, Sun-Hwa;Ryu, Hee-Wook;Cho, Kyung-Suk
    • Journal of Microbiology and Biotechnology
    • /
    • 제20권3호
    • /
    • pp.587-593
    • /
    • 2010
  • Three hundred and seventy-four rhizobacteria were isolated from the rhizosphere soil (RS) or rhizoplane (RP) of Echinochloa crus-galli, Carex leiorhyncha, Commelina communis, Persicaria lapathifolia, Carex kobomugi, and Equisetum arvense, grown in contaminated soil with petroleum and heavy metals. The isolates were screened for plant growth-promoting trait (PGPT), including indole acetic acid (IAA) productivity, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and siderophore(s) synthesis ability. IAA production was detected in 86 isolates (23.0%), ACC deaminase activity in 168 isolates (44.9%), and siderophore(s) synthesis in 213 isolates (57.0%). Among the rhizobacteria showing PGPT, 162 isolates had multiple traits showing more than two types of PGPT. The PGPT-possesing rhizobacteria were more abundant in the RP (82%) samples than the RS (75%). There was a negative correlation (-0.656, p<0.05) between the IAA producers and the ACC deaminase producers. Clustering analysis by principal component analysis showed that RP was the most important factor influencing the ecological distribution and physiological characterization of PGPT-possesing rhizobacteria.

연관지도를 이용한 새우난초, 금새우난초, 변이종의 화색의 유전분석 (Genetic Analysis of Flower Color Traits in Calanthe discolor, C. sieboldii, and Variants Using Molecular Linkage Map)

  • 조동훈;정미영;지선옥;김창길;정재동;김경민
    • 생명과학회지
    • /
    • 제19권9호
    • /
    • pp.1239-1244
    • /
    • 2009
  • 본 연구는 제주도에서 자생하는 새우난초 3개체, 금새우난초 3개체 그리고 변이종 14개체를 포함하여 총 20개체를 화색에 따라 분류하고 유전자지도를 작성하여 QTL분석을 하였다. 화색은 새우난초가 어두운 자색으로 CIE Lab값이 $40{\sim}50$ 정도였으며, 금새우난초는 황색으로 $110{\sim}130$ 정도였고, 변이종 개체들은 새우난초와 유사하거나 다소 높았다. PCR 결과 얻은 polymorphism이 인정되는 154개 marker에 대한 분리비 적합도 검정에서 51개 marker에서 5% 수준의 유의성이 인정되었으며, 유의성이 인정된 51개 marker 중에서 새우난초 type은 37개, 금새우난초 type은 14개 였다. Polymorphism이 인정된 154개 marker에 대하여 MAPL program을 이용하여 이들 marker 상호간의 연관관계를 분석한 결과는 16개의 연관군과 1개의 독립군으로 구분되었으며, 이들 연관군에 대한 분자연관지도는 전체 group의 크기가 220.4 cM (centi Morgan)이고, marker간의 평균거리는 3.3 cM이었다. 양적 형질에 대한 분자연관지도상의 QTL 분석 결과, LOD 3.0 이상인 화색과 설판색의 QTL은 각각 3개와 1개였다. 이상에서 얻어진 자료는 새우난초 속의 화색 연구에 도움이 될 것으로 사료된다.

Application of simple and massive purification system of dsRNA in vivo for acute toxicity to Daphnia magna

  • CHOI, Wonkyun;LIM, Hye Song;KIM, Jin;RYU, Sung-Min;LEE, Jung Ro
    • Entomological Research
    • /
    • 제48권6호
    • /
    • pp.533-539
    • /
    • 2018
  • The RNA interference (RNAi) has been considered as an important genetic tool and applied to develop a new living modified (LM) crop trait which is an improvement of nutrient quality or pest management. The RNAi of DvSnf7 has been used for resistance to LM maize and the Western Corn Rootworm which is a major agricultural pest for the US Corn Belt. Most of the environmental risk assessments (ERA) of double strand RNA (dsRNA) have been performed using in vitro transcript products, and not in vivo expressed product. A large amount of dsRNA was required for the acute toxicity assay of water fleas. Therefore development of massive dsRNA purification techniques is critical. Daphnia, a freshwater microcrustacean, is a model organism for studying cellular and molecular mechanism involved in life history traits and ecotoxicology. In this study, we established the massive dsRNA purification method using Escherichia coli and implemented acute toxicity assays to Daphnia magna. As a result, the present RNase A and DNase I, dsRNA was efficiently purified without any special techniques or equipment. Even though purified dsRNA existed during the acute toxicity test, lethality or abnormal behavior were not observed in D. magna. These results indicated that GFP and DvSnf7 dsRNA were not significantly affected to D. magna due to their lack of sequence matching in its genome. The purification method of dsRNA and the acute toxicity assay of water fleas using purified dsRNA would be suitable for the toxicological studies of LMOs to aquatic non-target organisms.

토양수분구배에서 굴참나무와 떡갈나무의 생육반응, 생태 지위 및 중복역 (Growth Response, Ecological Niche and Overlap between Quercus variabilis and Quercus dentata under Soil Moisture Gradient)

  • 박여빈;김의주
    • 한국환경복원기술학회지
    • /
    • 제26권5호
    • /
    • pp.47-56
    • /
    • 2023
  • The Quercus variabilis and Quercus dentata, which are said to be relatively drought tolerant among the important genus Quercus that represent deciduous broad-leaved forests in Korea. These two species are widely distributed worldwide in Korea, Japan and China (northern, central, western and eastern subtropical regions). This study compared the ecological niche breadth and overlap according to growth response in 4 soil moisture gradients for the two species and tried to reveal degree of competition and ecological niche characteristics. The ecological niche breadth was 0.977±0.020 for Q. variabilis and 0.979±0.014 for Q. dentata, the latter being slightly wider. And they were similar in 5 traits (stem length, leaf lamina length, leaf width length, stem weight, leaf petiole weight), Q. variabilis was more dominant in 4 traits (leaves number, stem diameter, leaf area, leaf petiole length), and Q. dentata was more dominant in 7 traits (root length, shoot length, plant weight, root weight, shoot weight, leaf weight, leaf petiole weight). The ecological niche overlap for soil moisture between the two species overlapped most in plant structure-related traits and least in photosynthetic organ-related traits such as petiole length. As a result of principal component analysis, degree of competition between the two species for soil moisture was more severe when the soil moisture condition was low than high. Among the measured traits that affect the two-dimensional distribution, 8 traits (Leaves number, Shoot length, Stem length, Plant weight, Root weight, Shoot weight, Stem weight, Leaves weight) were correlated with the factor 1, and 2 traits (Leaf width length, Leaf petiole weight) were correlated with the factor 2 (r>0.5). These results show that the ecological response of the two species to soil moisture is not a few traits involved, but several traits are involved simultaneously.

A Study on Receptivity to Sharing Living Space in Communal Shared Housing of the Elderly Living in Rural Areas depending on Personal Traits

  • Kim, Hyun-Jung;Lee, Yeun-Sook;An, So-Mi
    • KIEAE Journal
    • /
    • 제16권4호
    • /
    • pp.5-20
    • /
    • 2016
  • Purpose: The objective of this study is to divide personal traits of the elderly living in a rural area into extraversion, agreeableness, openness, conscientiousness, neuroticism, and loneliness and to identify the relationship between personal traits and receptivitiy to sharing living space in communal shared housing. Method: Subjects of this study are the elderly of ages greater than 55 living in Yeongwol-gun, Gangwon-do. Depending on how often elderly welfare facility was used, places where the elderly gathered were divided into a senior citizen center, senior welfare center, and other places where they often gathered. The researchers visited each of the places directly and conducted a survey with face-to-face interviews. Result: The collected data consisting of 124 respondents were analyzed through SPSS statistical program. It showed that 5 personal traits, except for agreeableness, had statistically significant difference. Extrovert and low lonely elderly people had high receptivity. The relationship between personal traits and acceptable shared space revealed differently depending on the function of space. Especially, shared resting space was related to low emotion-oriented trait, such as neuroticism and loneliness, while shared hobby and sanitary space were related to strong management-oriented trait of conscientiousness. These findings demonstrate the importance of understanding personal traits in predicting receptivitiy to sharing living space. Also, it is necessary to compare the degree of receptivity to sharing living space based on personal traits and to plan shared space in several levels, such as full sharing, partial sharing, and individual use, to develop and supply communal shared housing successfully.

한국 서울 식생의 번성자로서 팥배나무의 형질 변화 양상 (Changes in Leaf and Reproductive Traits of Mountain Ash (Sorbus alnifolia) as Urban Flourisher in the Seoul Metropolitan, South Korea)

  • 정성희;조용찬;이창석
    • 한국환경생태학회지
    • /
    • 제35권6호
    • /
    • pp.644-658
    • /
    • 2021
  • 도시 생태계에서 우점 수종의 형질 특성은 분획화 및 고립된 식생, 그리고 생물상호작용 감소 등 환경 변화에 대한 종의 형질 적응과 생태계 기능에 대한 통찰력을 제공한다. 우리는 한국의 서울 중심에서 남쪽 방향의 4개 산지에서 환경 요인 (지리, 기상 및 토양 속성)을 포함하여 모두 40개체 팥배나무 (Sorbus alnifolia)에서 측정한 4개 유형의 잎 형질 (Leaf area, Specific leaf area, Leaf dry mass content, Leaf shape index)과 7개 유형의 번식 형질 (Fruit width, Fruit length, Fruit shape, Fruit dry weight, Fruit dry matter content, Seed weight 및 Seed ratio)의 평균과 분산 양상, 그리고 형질 조합에 대한 다변량 분석을 실시하였다. 측정된 환경 요인은 조사 장소의 개별성을 나타내었지만, 도심 식생이 더 건조하고 생장도일이 길었다. 도심에서 팥배나무의 잎은 작고 무거워지며, 열매는 길쭉하고 가벼운 종자를 생산하여, 오랜 도시화 영향에 따른 형질 반응들이 관찰되었다. 우리의 연구는 식생 파편화 및 고립화에 따른 생물 상호작용 감소를 나타내는 도심 환경에서 팥배나무 집단의 성장 및 번식 기제의 변화를 확인하였다. 우리는 축소된 생물 상호작용 규모, 그리고 대기 오염 같은 부정적 환경 요인이 많은 도시에서 핵심 수종의 기능과 지속성에 관해 제한적이지만 뚜렷한 생태 정보를 제공한다.

기후변화 조건에서 수분구배 및 영양소 구배에 따른 굴참나무와 상수리나무 잎 형태적 특성의 생태지위 변화 (Variations in Ecological Niche of Quercus variabilis and Quercus acutissima Leaf Morphological Characters in Response to Moisture and Nutrient Gradient Treatments under Climate Change Conditions)

  • 박여빈;김의주;박재훈;김윤서;박지원;이정민;유영한
    • 한국환경복원기술학회지
    • /
    • 제27권2호
    • /
    • pp.43-53
    • /
    • 2024
  • This study attempted to elucidate the ecological niches and influencing environmental factors of Quercus variabilis and Quercus acutissima, which are representative deciduous broad-leaved trees in Korean forests, taxonomically close and genetically similar, under climate change conditions. Under climate change conditions induced by increased CO2 and temperature, soil moisture and nutrient environments were manipulated in four gradients. At the end of the growing, plants were harvested to measure growth responses, calculate ecological niches, and compare them with those of the control. Eperimental plants were grown for 180 days in a glass greenhouse designed with four gradients each for soil moisture and nutrient environments under climate change conditions induced by increased CO2 and temperature. After harvesting, growth responses of leaf traits were measured, ecological niches were calculated, and these were compared with those of the control groups. Furthermore, the responses of the two species' populations were interpreted using principal component analysis(PCA) based on leaf trait measurements. As a result, under climate change conditions, the ecological niche breadth for moisture environment was broader for Quercus variabilis than Quercus acutissima, whereas for the nutrient environment, Quercus acutissima exhibited a broader niche breadth than Quercus variabilis. And the rate of change in ecological niche breadth due to climate change decreased for Quercus variabilis in both moisture and nutrient environments, while for Quercus acutissima, it increased in the moisture environment but decreased in the nutrient environment. Additionally, in terms of group responses, both Quercus variabilis and Quercus acutissima expanded their ecological niches under climate change conditions in both soil moisture and nutrient conditions, with Quercus acutissima exhibiting a broader niche than Quercus variabilis under nutrient conditions. These results indicate that the changes in leaf morphological characteristics and the responses of individuals reflecting them vary not only under climate change conditions but also depending on environmental factors.

전남 서남부 농촌지역에 분포하는 나비군집의 변화 양상에 관한 연구 (Pattern of Change of the Local Butterfly Community in a Rural Area of Southwestern Part of Korea)

  • 최세웅;안정섭
    • 환경생물
    • /
    • 제33권1호
    • /
    • pp.53-62
    • /
    • 2015
  • 농촌 논과 밭 경작지 및 야산지역에 분포하는 나비군집 변화가 지난 10년 동안 어떻게 변화했는가를 알아보기 위하여 2001년부터 2002년에 이루어진 자료와 2014년 자료를 비교, 분석하였다. 나비 조사는 선 조사법을 통하여 동일한 지점에서 이루어졌다. 조사 결과 나비의 종 수와 개체수는 10년 전과 비교하였을 때 줄어드는 양상을 나타내었는데 2001년과 2002년에 비하여 2014년에는 종 수와 개체수 모두 감소하였다. 조사지역의 출현 종 수가 그들의 생태학적 특성인 서식지, 서식지 범위, 먹이식물, 먹이식물 범위, 세대수 및 겨울나기 유형 등에 변화가 있는 가를 조사한 결과 종 수는 차이가 나타나지 않았으나 개체수에서는 겨울나기 유형을 제외하고 차이가 있는 것을 확인할 수 있었다. 이 결과는 지난 10년간 지역적으로 나비 군집에 많은 변화가 있었다는 것을 나타내고 있으며 주 원인은 조사지역의 서식지 변화가 주요 요인이었을 것으로 추측한다. 조사 지역에서 나비 군집의 변화를 일으키는 원인을 알기 위해서는 앞으로 지역적 수준에서 종 변화 및 이 변화의 원인을 알기 위해서는 지속적인 모니터링이 필요할 것으로 생각한다.

애기장대 H+-PPase(AVP1) 과발현 배추에서 바이오매스 증가와 내염성 향상 (Increased biomass and enhanced tolerance to salt stress in Chinese cabbage overexpressing Arabidopsis H+-PPase (AVP1))

  • 박미희;원희연;김창길;한증술
    • Journal of Plant Biotechnology
    • /
    • 제39권4호
    • /
    • pp.253-260
    • /
    • 2012
  • 애기장대 액포 소재 $H^+$-PPase(AVP1)의 과발현이 농업적으로 가치 있는 표현형을 나타낸다는 기 보고에 기초하여, AVP1 발현이 다른 종에서도 일관되게 바이오매스를 증가시키고 염에 대한 내성을 향상시키는지를 확인하기 위하여 본 연구에서는 AVP1 형질전환 배추 식물체를 획득한 후 고정계통을 육성하여 생리검정 재료로 사용하였다. 형질전환 배추 유식물체는 비형질전환 유식물체에 비해 생장이 왕성하였으며 염스트레스에 대한 내성도 강하였다. 정상 재배조건에서 생장시킨 유식물체의 생체중과 건물중을 비교함으로써 형질전환에 의한 바이오매스증가 표현형을 확인하였으며 MS 염과 NaCl로 점차 염스트레스를 강화시키는 조건에서 광계II 양자수율을 추적, DAB 염색 실시 및 최종적으로 용토 탈염 후 회복 실험을 수행함으로써 내염성 향상 표현형을 확인하였다.