Horticultural Science & Technology (원예과학기술지)

Korean Society of Horticultural Science (한국원예학회)
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Classification of Allium monanthum and A. grai by ISSR Markers

ISSR 마커를 이용한 달래와 산달래의 분류

  • Lee, Sais-Beul (School of Applied Ecological Resources, Kyungpook National University) ;
  • Kim, Chang-Kil (School of Applied Ecological Resources, Kyungpook National University) ;
  • Oh, Jung-Yeol (School of Applied Ecological Resources, Kyungpook National University) ;
  • Kim, Kyung-Min (School of Applied Ecological Resources, Kyungpook National University)
  • 이샛별 (경북대학교 생태자원응용학부) ;
  • 김창길 (경북대학교 생태자원응용학부) ;
  • 오중열 (경북대학교 생태자원응용학부) ;
  • 김경민 (경북대학교 생태자원응용학부)
  • Received : 2011.04.04
  • Accepted : 2011.07.18
  • Published : 2011.12.31
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Abstract

One hundred twenty two accessions of 6 species in genus Allium were collected throughout 5 regions of Korea. Their genetic relationship was investigated by using inter simple sequence repeat (ISSR) markers. The morphological analysis was measured for 6 quantitative and quantified for 1 qualitative trait. ISSR analysis obtained a total of 370 polymorphic bands by using seventeen primers. The cluster analysis of genus Allium based on morphological data could identify three groups. The accessions of Allium belonged to the Allium monanthum clustered into five groups at genetic distance ranging from 0.94 on the base of ISSR analysis. Correlation analysis between morphological and ISSR analysis showed low coefficient(r = 0.036). These markers are thought to be used in research of molecular markers for classification and cross breeding of Allium monanthum and A. grai.

Allium속에 포함된 6종의 122점을 수집하고, 이 종들의 유전적 관계는 ISSR 마커를 이용하여 확인하였다. 형태적 분석은 6개의 양적 형질을 측정하고 1개의 질적 형질은 수치화하였다. SSR 분석은 17개의 primer를 사용하여 총 370개의 다형성 밴드를 얻었다. 형태적 특성 분석은 유전적 거리로 구분할 경우 3개의 그룹으로 분류되었으나, 부분적으로 몇 몇 종들은 분류에 어려움이 있었다. ISSR 결과를 바탕으로 Allium 속의 군집분석은 5개의 그룹으로 분리되었다. 형태적 분석과 SSR분석 간의 상관 관계는 유의성이 매우 낮았다(r = 0.036). 따라서 본 연구에서 개발한 ISSR 마커는 달래와 산달래의 분류와 교배 육종에 유용하게 이용될 수 있을 것이다.

Keywords

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