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http://dx.doi.org/10.5352/JLS.2009.19.9.1239

Genetic Analysis of Flower Color Traits in Calanthe discolor, C. sieboldii, and Variants Using Molecular Linkage Map  

Cho, Dong-Hoon (Industry Support Group, Ulsan Technopark)
Chung, Mi-Young (School of Applied Biosciences, Kyungpook National University)
Jee, Sun-Ok (Department of Cosmetic Science, Joongbu University)
Kim, Chang-Kil (School of Applied Ecological Resources, Kyungpook National University)
Chung, Jae-Dong (School of Applied Biosciences, Kyungpook National University)
Kim, Kyung-Min (School of Applied Ecological Resources, Kyungpook National University)
Publication Information
Journal of Life Science / v.19, no.9, 2009 , pp. 1239-1244 More about this Journal
Abstract
This study was conducted to clarify the genetic relationship between Calanthe discolor, C. sieboldii and variants, and the cause of flower color variations by using a molecular linkage map and a quantitative trait loci (QTL) analysis for flower and lip color in Calanthe species native to Korea. Twenty plants were included in three C. discolor and three C. sieboldii, and fourteen variants were obtained from their habitat, Jeju-do in Korea. The flowers of C. discolor were brownish red, the values of Commission Internationale de I'Eclairage (CIE) Lab were between 40 and 50. The flowers of C. sieboldii were yellowish, the values of CIE Lab were between 110 and 130. The variants had various mixed colors that were thought to have originated from natural hybridization between C. discolor and C. sieboldii, and the values of CIE Lab were between 50 and 70. The colors of the lips were usually divided into white and yellow. C. discolor had a white lip, C. sieboldii had a yellow one, and the variants had a white to yellow one. The CIE Lab value of each color was 90 in white and 110 to 120 in yellow lips. A molecular linkage mapping was constructed based on the segregation of 154 RAPD markers using a MAPL program. Sixteen linkage groups containing 66 markers were established. It covered a total map distance of 220.4 cM. The distance between adjacent markers ranged from 0 to 6.6 cM, with an average distance of 3.3 cM. These markers are thought to be closely associated with flower and lip color expression. Among the 16 molecular linkage groups, 3 QTLs had flower color trait loci and 1 QTL had lip color trait loci.
Keywords
Calanthe; molecular linkage map; quantitative trait loci (QTL); flower color;
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