• Title/Summary/Keyword: eIF5B

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Construction and Characterization of Vector Expressing Low Level of Translation Factor eIF5B (단백질합성인자 eIF5B의 저 발현 효모벡터의 제조 및 특성)

  • 최상기;송진희;이준행;이병욱;성치남
    • Korean Journal of Microbiology
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    • v.40 no.1
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    • pp.7-11
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    • 2004
  • eIF5B is a translation initiation factor that delivers Met-$tRNA^{Met}$ to AUG start codon and subsequently joins the small and large ribosomes. In order to study the function of eIF5B encoded by FUN12, we constructed FUN12 which lacked 5' end of its sequence. We found that this construct lacking almost all of its promoter in pRS plasmid partially complemented slow growth phenotype of fun12 deletion strain. Interestingly, this construct expressed N-terminally truncated eIF5B and its expression level was about 5% of that of wild type eIF5B. Low amount of the eIF5B expressed additionally in fun12 deletion strain played a direct role as a limiting factor for its growth. This limiting factor eIF5B in those strains also modulates activities of overall translation in vitro.

Functional Equivalence of Translation Factor elF5B from Candida albicans and Saccharomyces cerevisiae

  • Jun, Kyung Ok;Yang, Eun Ji;Lee, Byeong Jeong;Park, Jeong Ro;Lee, Joon H.;Choi, Sang Ki
    • Molecules and Cells
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    • v.25 no.2
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    • pp.172-177
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    • 2008
  • Eukaryotic translation initiation factor 5B (eIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, and promotes joining of the 60S ribosomal subunit. To see whether the eIF5B proteins of other organisms function in Saccharomyces cerevisiae, we cloned the corresponding genes from Oryza sativa, Arabidopsis thaliana, Aspergillus nidulans and Candida albican and expressed them under the control of the galactose-inducible GAL promoter in the $fun12{\Delta}$ strain of Saccharomyces cerevisiae. Expression of Candida albicans eIF5B complemented the slow-growth phenotype of the $fun12{\Delta}$ strain, but that of Aspergillus nidulance did not, despite the fact that its protein was expressed better than that of Candida albicans. The Arabidopsis thaliana protein was also not functional in Saccharomyces. These results reveal that the eIF5B in Candida albicans has a close functional relationship with that of Sacharomyces cerevisiae, as also shown by a phylogenetic analysis based on the amino acid sequences of the eIF5Bs.

A Study on the Implementation and Performance Analysis of FPGA Based Galileo E1 and E5 Signal Processing (FPGA 기반의 갈릴레오 E1 및 E5 신호 처리 구현 및 성능에 관한 연구)

  • Sin, Cheon-Sig;Lee, Sang-Uk;Yoon, Dong-Weon;Kim, Jae-Hoon
    • Journal of Satellite, Information and Communications
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    • v.4 no.1
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    • pp.36-44
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    • 2009
  • The key technologies of GNSS receiver for GNSS sensor station are under development as a part of a GNSS ground station in ETRI. This paper presents the GNSS receiver implementation and signal processing result which is implemented based on FPGA to process the Galileo E1 and E5 signal. To verify the working and performance for GNSS receiver which is implemented based on FPGA, live signal received from GIOVE-B which is second test satellite is used. We gather GIOVE-B signal by using prototyping antenna and RF/IF units including IF-component. To verify Galileo E1 and E5 signal processing function from GIOVE-B, FPGA based signal processing module is implemented as a prototyping hardware board.

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An Interferon Resistance Induced by the Interaction between HCV NS5B and Host p48 (C형 간염 바이러스 NS5B 단백질과 숙주의 p48 단백질의 상호작용에 의한 인터페론 저항성의 유도)

  • Park, So-Yeon;Lee, Jong-Ho;Myung, Hee-Joon
    • Microbiology and Biotechnology Letters
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    • v.36 no.4
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    • pp.353-359
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    • 2008
  • Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.

NEIGHBORHOOD CONDITION AND FRACTIONAL f-FACTORS IN GRAPHS

  • Liu, Hongxia;Liu, Guizhen
    • Journal of applied mathematics & informatics
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    • v.27 no.5_6
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    • pp.1157-1163
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    • 2009
  • Let G be a graph with vertex set V(G) and let f be a nonnegative integer-valued function defined on V(G). A spanning subgraph F of G is called a fractional f-factor if $d^h_G$(x)=f(x) for all x $\in$ for all x $\in$ V (G), where $d^h_G$ (x) = ${\Sigma}_{e{\in}E_x}$ h(e) is the fractional degree of x $\in$ V(F) with $E_x$ = {e : e = xy $\in$ E|G|}. In this paper it is proved that if ${\delta}(G){\geq}{\frac{b^2(k-1)}{a}},\;n>\frac{(a+b)(k(a+b)-2)}{a}$ and $|N_G(x_1){\cup}N_G(x_2){\cup}{\cdots}{\cup}N_G(x_k)|{\geq}\frac{bn}{a+b}$ for any independent subset ${x_1,x_2,...,x_k}$ of V(G), then G has a fractional f-factor. Where k $\geq$ 2 be a positive integer not larger than the independence number of G, a and b are integers such that 1 $\leq$ a $\leq$ f(x) $\leq$ b for every x $\in$ V(G). Furthermore, we show that the result is best possible in some sense.

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A Study on the Performance Improvement of Turbo Coded OFDM Systems Considering Frequency Offset (주파수 오프셋을 고려한 Tued OFDM 시스템의 성능 개선에 관한 연구)

  • 이영춘;박기식
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.5 no.5
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    • pp.908-915
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    • 2001
  • In this paper, it is analyzed theoretically that the performance degradation, caused by carrier frequency offset, in an OFDM/M-ary PSK system. Then, when Turbo coding is adopted to an OFDM/M-ary PSK system, the degree of performance enhancement is evaluated. Finally, the maximum frequency offset is calculated to satisfy the BER performance required in a Turbo coded OFDM/M-ary PSK system. As results of analysis, it is shown that the more the number of M-ary is, the worse the BER performance is. Moreover, 7dB, 9dB, and 17dB of $E_b/N_o$ are required in QPSK, 8PSK and 16PSK systems, respectively in order to satisfy the error performance, $BER=10^{-3}$ for voice communication. If $E_b/N_o$ are 10㏈ and 15㏈, the frequency offset should be below 0.05 and 0.075, respectively, for voice communication. When Turbo coding is adopted to an OFDM/M-ary PSK system, the less the number of M-ary is, the greater the performance enhancement of Turbo coding is. If the number of a M-ary system of the system is below 16, it is found that required $E_b/N_o$ is about 8dB to satisfy $BER=10^{-5}$ Moreover, in the system the Turbo coding scheme, voice communication is available with greatly low$E_b/N_o$, and 8dB of $E_b/N_o$ is enough for data communication regardless of the permission range of frequency offset.

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A NOTE ON WITT RINGS OF 2-FOLD FULL RINGS

  • Cho, In-Ho;Kim, Jae-Gyeom
    • Bulletin of the Korean Mathematical Society
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    • v.22 no.2
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    • pp.121-126
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    • 1985
  • D.K. Harrison [5] has shown that if R and S are fields of characteristic different from 2, then two Witt rings W(R) and W(S) are isomorphic if and only if W(R)/I(R)$^{3}$ and W(S)/I(S)$^{3}$ are isomorphic where I(R) and I(S) denote the fundamental ideals of W(R) and W(S) respectively. In [1], J.K. Arason and A. Pfister proved a corresponding result when the characteristics of R and S are 2, and, in [9], K.I. Mandelberg proved the result when R and S are commutative semi-local rings having 2 a unit. In this paper, we prove the result when R and S are 2-fold full rings. Throughout this paper, unless otherwise specified, we assume that R is a commutative ring having 2 a unit. A quadratic space (V, B, .phi.) over R is a finitely generated projective R-module V with a symmetric bilinear mapping B: V*V.rarw.R which is nondegenerate (i.e., the natural mapping V.rarw.Ho $m_{R}$ (V, R) induced by B is an isomorphism), and with a quadratic mapping .phi.:V.rarw.R such that B(x,y)=(.phi.(x+y)-.phi.(x)-.phi.(y))/2 and .phi.(rx)= $r^{2}$.phi.(x) for all x, y in V and r in R. We denote the group of multiplicative units of R by U(R). If (V, B, .phi.) is a free rank n quadratic space over R with an orthogonal basis { $x_{1}$, .., $x_{n}$}, we will write < $a_{1}$,.., $a_{n}$> for (V, B, .phi.) where the $a_{i}$=.phi.( $x_{i}$) are in U(R), and denote the space by the table [ $a_{ij}$ ] where $a_{ij}$ =B( $x_{i}$, $x_{j}$). In the case n=2 and B( $x_{1}$, $x_{2}$)=1/2, we reserve the notation [ $a_{11}$, $a_{22}$] for the space.the space.e.e.e.

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Odd Harmonious and Strongly Odd Harmonious Graphs

  • Seoud, Mohamed Abdel-Azim;Hafez, Hamdy Mohamed
    • Kyungpook Mathematical Journal
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    • v.58 no.4
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    • pp.747-759
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    • 2018
  • A graph G = (V (G), E(G) of order n = |V (G)| and size m = |E(G)| is said to be odd harmonious if there exists an injection $f:V(G){\rightarrow}\{0,\;1,\;2,\;{\ldots},\;2m-1\}$ such that the induced function $f^*:E(G){\rightarrow}\{1,\;3,\;5,\;{\ldots},\;2m-1\}$ defined by $f^*(uv)=f(u)+f(v)$ is bijection. While a bipartite graph G with partite sets A and B is said to be bigraceful if there exist a pair of injective functions $f_A:A{\rightarrow}\{0,\;1,\;{\ldots},\;m-1\}$ and $f_B:B{\rightarrow}\{0,\;1,\;{\ldots},\;m-1\}$ such that the induced labeling on the edges $f_{E(G)}:E(G){\rightarrow}\{0,\;1,\;{\ldots},\;m-1\}$ defined by $f_{E(G)}(uv)=f_A(u)-f_B(v)$ (with respect to the ordered partition (A, B)), is also injective. In this paper we prove that odd harmonious graphs and bigraceful graphs are equivalent. We also prove that the number of distinct odd harmonious labeled graphs on m edges is m! and the number of distinct strongly odd harmonious labeled graphs on m edges is [m/2]![m/2]!. We prove that the Cartesian product of strongly odd harmonious trees is strongly odd harmonious. We find some new disconnected odd harmonious graphs.

Design of RF Front-end for High Precision GNSS Receiver (고정밀 위성항법 수신기용 RF 수신단 설계)

  • Chang, Dong-Pil;Yom, In-Bok;Lee, Sang-Uk
    • Journal of Satellite, Information and Communications
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    • v.2 no.2
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    • pp.64-68
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    • 2007
  • This paper describes the development of RF front.end equipment of a wide band high precision satellite navigation receiver to be able to receive the currently available GPS navigation signal and the GALILEO navigation signal to be developed in Europe in the near future. The wide band satellite navigation receiver with high precision performance is composed of L - band antenna, RF/IF converters for multi - band navigation signals, and high performance baseband processor. The L - band satellite navigation antenna is able to be received the signals in the range from 1.1 GHz to 1.6 GHz and from the navigation satellite positioned near the horizon. The navigation signal of GALILEO navigation satellite consists of L1, E5, and E6 band with signal bandwidth more than 20 MHz which is wider than GPS signal. Due to the wide band navigation signal, the IF frequency and signal processing speed should be increased. The RF/IF converter has been designed with the single stage downconversion structure, and the IF frequency of 140 MHz has been derived from considering the maximum signal bandwidth and the sampling frequency of 112 MHz to be used in ADC circuit. The final output of RF/IF converter is a digital IF signal which is generated from signal processing of the AD converter from the IF signal. The developed RF front - end has the C/N0 performance over 40dB - Hz for the - 130dBm input signal power and includes the automatic gain control circuits to provide the dynamic range over 40dB.

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Almost derivations on the banach algebra $C^n$[0,1]

  • Jun, Kil-Woung;Park, Dal-Won
    • Bulletin of the Korean Mathematical Society
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    • v.33 no.3
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    • pp.359-366
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    • 1996
  • A linear map T from a Banach algebra A into a Banach algebra B is almost multiplicative if $\left\$\mid$ T(fg) - T(f)T(g) \right\$\mid$ \leq \in\left\$\mid$ f \right\$\mid$\left\$\mid$ g \right\$\mid$(f,g \in A)$ for some small positive $\in$. B.E.Johnson [4,5] studied whether this implies that T is near a multiplicative map in the norm of operators from A into B. K. Jarosz [2,3] raised the conjecture : If T is an almost multiplicative functional on uniform algebra A, there is a linear and multiplicative functional F on A such that $\left\$\mid$ T - F \right\$\mid$ \leq \in', where \in' \to 0$ as $\in \to 0$. B. E. Johnson [4] gave an example of non-uniform commutative Banach algebra which does not have the property described in the above conjecture. He proved also that C(K) algebras and the disc algebra A(D) have this property [5]. We extend this property to a derivation on a Banach algebra.

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