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An Interferon Resistance Induced by the Interaction between HCV NS5B and Host p48  

Park, So-Yeon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Lee, Jong-Ho (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Myung, Hee-Joon (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Publication Information
Microbiology and Biotechnology Letters / v.36, no.4, 2008 , pp. 353-359 More about this Journal
Abstract
Hepatitis C virus (HCV) is known as the causative agent of blood transmitted hepatitis. Two viral proteins, E2 and NS5A, are known to exert interferon resistance of HCV via PKR pathway. Here, we report a third protein, the RNA-dependent RNA polymerase (NS5B) of HCV, induced interferon resistance inhibiting p56 pathway. p56 was shown to interact with p48 subunit of eukaryotic initiation factor 3 (eIF3). This interaction inhibited formation of ternary complex in translation initiation. Using dual reporter assay system, we observed that the translation decreased when interferon alpha was added to the culture. But, in the presence of HCV NS5B, the translation partly recovered. NS5B and p48 subunit of eIF3 were shown to interact. This interaction seems to inhibit the interaction between p48 and p56. This is the first report that a virus exerts interferon resistance via p56 pathway.
Keywords
HCV; NS5B; p48; p56; interferon resistance;
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