• 대한한의학회지
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• 제29권2호
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• pp.41-46
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• 2008

Objective: To build a basis of strategy for development of herbal drug as a preventive medicine during dynamic innovation of Korean Oriental medicine. Methods: Via telephone interview, 101 subjects prescribed with deer antler were asked to provide us their reflections about five topics: points of satisfaction, physical improvement, improved symptoms, annual times of taking restorative herbal medicine, and aspect of complaints about taking the herbal drugs. Results: Around 68% of subjects usually take a restorative herbal medicine with deer antler less than once per year. 63% of subjects were satisfied with herbal restoratives. They answered the physical energetic feeling (52%) as the most benefit while expensive price (43%) as the most frequent complaint. Conclusion: Scientifically designed restorative herbal medicine needs to be developed for competitiveness of Korean Oriental medicine as a preventive medicine. In addition, more reasonable price and confirmative data for safety and quality control should be equipped in Korean Oriental medical market.

• 한국임상수의학회지
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• 제14권2호
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• pp.254-257
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• 1997

The blood components of not-young (young antler) were examined in the Red Deer. Blood samples were collected from the antler and jugular veins respectively. The hematological and serum chemical values were as follows; 1. RBC count of not-hyul (young antler blood) was significantly higher than that of jugular vein blood (p<0.05). However, fibrinogen concentration of rook hyul was significantly lower than that of jugular vein blood (p<0.01). WBC counts packed cell volume (PCV) and total protein fro) were not significant between nolo-hyul and jugular vein blood. 2. The concentration of phosphorous and magnesium of not-hyul were significantly higher than those of jugular vein blood (p<0.05). Albumin and glutamic oxaloacetic transaminase (GOT) of nolo-hyul were higher than those of jugular vein blood. But globulin and calcium concentration of not-hyul were lower than those of jugular vein blood. Blood urea nitrogen BUN) of rook-hyul was not significant when compared with that of jugular vein blood.

• 대한한의학회지
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• 제43권3호
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• pp.139-149
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• 2022

Objectives: This study was designed to investigate the clinical effectiveness of Ikgibohyeol-tang(Yìqìbǔxuè-tāng) granules and Deer antler granules on fatigue symptoms. Methods: This is a 39-patient case series from a retrospective chart review of outpatients who were treated with Ikgibohyeol-tang granules and Deer antler granules at a Kyung Hee University Korean Medicine Hospital (Seoul) between 1st, January 2015 to 1st, August 2021. We reviewed the patients who complained of fatigue and analyzed changes in the degree of discomfort with fatigue and accompanying symptoms and HRV (Heart Rate Variability) score before and after treatment. Results: After taking Ikgibohyeol-tang granules and Deer antler granules, the degree of discomfort with fatigue and accompanying symptoms decreased significantly compared to before taking them. After treatment, Autonomic nervous system activity, Stress resistance, Heart Stability increased, and Stress index, Degree of fatigue decreased significantly. Conclusions: This study showed subjective symptoms and objective indicator (HRV score) were improved by taking Ikgibohyeol-tang granules and Deer antler granules.

• KSBB Journal
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• 제27권4호
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• pp.243-250
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• 2012

The ash content, mineral composition, total phenolics, antioxidant capacity, and neuroprotective effect of the antlers of deer fed with herb-incorporated feedstuff (HFS) or normal feedstuff (NFS) were comparatively evaluated. The contents of ash and mineral of the antler of deer fed with HFS were significantly lower than its counterpart. The ash and mineral contents of deer antlers decreased generally from the upper section toward the tip section. The ratios of Ca/ash, Ca/P, and Ca/Fe of antler of deer fed with HFS were lower than those of antler of deer fed with NFS. Antlers of deer fed with HFS had higher total phenolics, nitrite scavenging capacity, and antioxidant capacity than those of deer fed with NFS. Antlers of deer fed with HFS or NFS showed in vitro neuronal protection of PC-12 cells against oxidative stress in a dose-dependent manner, where antler of deer fed with HFS generally had higher cellular viability than NFS. These results above suggest that the incorporation of the medicinal herbal complex into feedstuff may improve the biological effects of deer antlers.

• 한국식품영양학회지
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• 제20권2호
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• pp.114-119
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• 2007

Our research objective was to examine the in vitro biological activity of deer antler(Nogyong in Korean) extract, including the antioxidative, nitrite scavenging, and tyrosinase inhibitory effects, as well as the antithrombotic, and angiotensin I converting enzyme(ACE) inhibitory activities. The carbohydrate, protein, fat, and mineral contents of the deer antler were 7.6%, 65.3%, 3.2% and 23.9%, respectively. The electron donating ability(EDA) by the reduction of 2,2'-diphenyl-1-picrylhydrazyl(DPPH) was 67.1%, and the inhibition rate of lipid peroxidation by the thiocyanate method using linoleic acid was 92.1% in 100 mg/ml of extract. The nitrite scavenging effects were pH dependent, and were highest at pH 1.2 and lowest at pH 6.0. The sample inhibition rate against tyrosinase was above 64.0%. The platelet aggregation induced by ADP(adenosine-5'diphosphate) was inhibited up to 51.7%, and the inhibitory effect was dependent on the sample concentration. Lastly, the inhibition rate of ACE was 47.5% in 100 mg/ml of deer antler extract.

• 생약학회지
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• 제34권3호통권134호
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• pp.263-268
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• 2003

This study was carried out to evaluate the change of blood constituents during the growth of antlers in Korean spotted deer (Cervus nippon). Samples of blood, obtained from the jugular vein of twenty five deer (3 to 6 year-old males) were taken in 10 days interval from casting to cutting of antler and were analyzed the blood parameters. Total-protein concentration in blood serum showed significantly differences during growth period of velvet antler (p<0.01), and it had similar tendency for total protein and albumin concentration, but albumin concentration was about 40-45% of total protein concentration. Urea and creatinine concentration was tended to inverse proportion, and it was almost 30:1 for ratio of urea and creatinine concentration. The concentration of total protein, creatinine, and uric acid concentration in blood during growth period of velvet antler was similarly increased and decreased but urea concentration was opposed. The concentration of total-bilirubin and direct-bilirubin at casting was higher than those at other period(P<0.05).

• Natural Product Sciences
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• 제17권4호
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• pp.315-320
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• 2011

Routinely applicable HPLC assay procedures for the ganglioside content in various deer antler preparations were established through the creation of a UV-absorbing chromophoric substance - trans-${\alpha},{\beta}$-unsaturated-hexadecene-aldehyde - from the sphingosine moiety in ganglioside molecules by two step chemical reactions. In order to guarantee the assay's accuracy and sensitivity, the HPLC-assay procedure adopted internal reference procedures by mixing cis-${\alpha},{\beta}$-unsaturated-hexadecene aldehyde[V] or cis-3-heptadecene- 1,2-diol[IV] to assay samples. The internal reference compound [IV] or [V] was synthesized in our laboratory starting from mannitol-diacetonide through three or four step organic reactions. This new HPLC-assay procedure was successfully applied to deer antler extracts with good dose-dependent calibration curves at the picomole level of gangliosides.

• Journal of Animal Science and Technology
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• 제64권1호
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• pp.166-182
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• 2022

Deer antler velvet is widely used in traditional medicine for its anti-aging, antioxidant, and immunity-enhancing effects. However, few studies have reported on the discovery of probiotic strains for deer antler fermentation to increase functional ingredient absorption. This study evaluated the ability of probiotic lactic acid bacteria to enhance the concentrations of bioactive molecules (e.g., sialic acid and gamma-aminobutyric acid [GABA]) in extracts of deer antler velvet. Seventeen strains of Lactobacillus spp. that were isolated from kimchi and infant feces, including L. sakei, L. rhamnosus, L. brevis, and L. plantarum, and those that improved the life span of Caenorhabditis elegans were selected for evaluation. Of the 17 strains, 2 (L. rhamnosus LFR20-004 and L. sakei LFR20-007) were selected based on data showing that these strains increased both the sialic acid and GABA contents of deer antler extract after fermentation for 2 d and significantly improved the life span of C. elegans. Co-fermentation with both strains further increased the concentrations of sialic acid, GABA, and metabolites such as short-chain fatty acids and amino acids. We evaluated the biological effects of the fermented antler velvet (FAV) on the antibacterial immune response in C. elegans by assessing worm survival after pathogen infection. The survival of the C. elegans conditioned with FAV for 24h was significantly higher compared with that of the control worm group fed only normal feed (non-pathogenic E. coli OP50) exposed to E. coli O157:H7, Salmonella typhi, and Listeria monocytogenes. To evaluate the protective effects of FAV on immune response, cyclophosphamide (Cy), an immune-suppressing agent was treated to in vitro and in vivo. We found that FAV significantly restored viability of mice splenocytes and immune promoting-related cytokines (interleukin [IL]-6, IL-10, inducible nitric oxide synthase [iNOS], interferon [IFN]-γ, and tumor necrosis factor [TNF]-α) were activated compared to non-fermented deer antlers. This finding indicated the protective effect of FAV against Cy-induced cell death and immunosuppressed mice. Taken together, our study suggests that immune-promoting antler velvet can be produced through fermentation using L. rhamnosus LFR20-004 and L. sakei LFR20-007.

• Journal of Acupuncture Research
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• 제23권2호
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• pp.73-90
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• 2006

Objectives : The effects of water extract of deer antler herbal-acupunture solution(DHS), prepared from the pilose antler of Cervus korea TEMMINCK var. mantchuricus Swinhoe (Nokyong), a traditional immunosuppressive and immune-activating Korean herbal- acupuncture, on collagen-induced arthritis(CIA:RA model) in mice was studied. Destruction of cartilage and bone are hallmarks of human rheumatoid arthritis, and controlling these erosive processes is the most challenging objective in the treatment of RA. Methods : We investigated the tissue protective effects of deer antler treatment using established murine collagen-induced arthritis(CIA) as a model. Potential synergy of low dosages of anti-inflammatory glucocorticosteroids and deer antler was also evaluated. Results : Treatment of established murine CIA with deer antler herbal-acupunture solution(DHS) $(10-50{\mu}g/day)$ suppressed disease activity and protected against cartilage and bone destruction. Although $10-50{\mu}g/day$ DHS had only a moderate effect on the inflammatory component of the disease activity, it strongly reduced cartilage pathology, as determined by histological examination. Serum cartilage oligomeric matrix protein(COMP) levels were significantly reduced, confirming decreased cartilage involvement. Histological analysis showed that bone destruction was prevented. DHS administration increased serum IL-1Ra levels and reduced anticollagen type II antibody levels. Treatment with low-dose $DHS(1{\mu}g/day)$ was ineffective in suppressing disease score, serum COMP or joint destruction. Synergistic suppression of both arthritis oseverity and COMP levels was noted when low-dose DHS was combined with prednisolone(0.05mg/kg/day), however, which in itself was not effective. Conclusion : DHS was shown to have the inhibiting effects against $IL-1{\alpha}-$ and $IL-1{\beta}-stimulated$ bone resorption. These results indicated that the DAS is not only highly stable and applicable to clinical uses in bone resorption, but also it will be served as a potent anti-inflammatory and anti-arthritic agents for treatment of human RA.

• Journal of Animal Science and Technology
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• 제45권6호
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• pp.1031-1038
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• 2003

본 연구는 축사 내에서 집약적으로 사육되고 있는 꽃사슴의 녹용 성장기간 중의 혈액성분변화와 녹용성장과의 관련성 해석을 위한 기초자료를 제공하고자 수행하였다. 녹용성장기간 중 혈액성분의 변화를 측정하기 위하여 우리 내에서 사육 중인 4${\sim}$6년 생 꽃사슴 웅록 25두를 무작위로 선별하여, 낙각 직후부터 10일 간격으로 절각일(50일)까지 혈액을 채취하여 미네랄과 효소 활성치를 측정하였다. 칼슘과 인의 농도는 녹용성장기간 중 커다란 변동이 없었으며 나트륨, 칼륨, 염소의 농도는 낙각 직후와 녹용성장에 따른 경시적인 유의차가 나타났다. AST, ALT, amylase, CK, GGT 및 LDH의 농도는 낙각 직후와 녹용성장에 따른 유의차는 인정되지 않았으나, ALK-P의 농도는 녹용성장이 진행되면서 계속 증가하여 낙각 후 50일 경에는 최고치를 나타내어 낙각시의 농도와 유의차가 나타났으며(p〈0.01), CK와 LDH의 활성은 다른 동물들보다 높게 나타났다.