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A New HPLC-analytical Method for Total Sphingosine Contents as an Indirect Index for the Ganglioside Contents of Deer Antlers  

Choi, Hye-Ok (Life science Research Institute, ES-Biotech. Co. Ltd.)
Kim, Jeung-Won (Life science Research Institute, ES-Biotech. Co. Ltd.)
Jo, Sung-Jun (Life science Research Institute, ES-Biotech. Co. Ltd.)
Kim, Jung-Hwan (Life science Research Institute, ES-Biotech. Co. Ltd.)
Han, Byung-Hoon (Life science Research Institute, ES-Biotech. Co. Ltd.)
Publication Information
Natural Product Sciences / v.17, no.4, 2011 , pp. 315-320 More about this Journal
Abstract
Routinely applicable HPLC assay procedures for the ganglioside content in various deer antler preparations were established through the creation of a UV-absorbing chromophoric substance - trans-${\alpha},{\beta}$-unsaturated-hexadecene-aldehyde - from the sphingosine moiety in ganglioside molecules by two step chemical reactions. In order to guarantee the assay's accuracy and sensitivity, the HPLC-assay procedure adopted internal reference procedures by mixing cis-${\alpha},{\beta}$-unsaturated-hexadecene aldehyde[V] or cis-3-heptadecene- 1,2-diol[IV] to assay samples. The internal reference compound [IV] or [V] was synthesized in our laboratory starting from mannitol-diacetonide through three or four step organic reactions. This new HPLC-assay procedure was successfully applied to deer antler extracts with good dose-dependent calibration curves at the picomole level of gangliosides.
Keywords
HPLC-sphingosine; deer antler; ossified deer antler; ${\alpha},{\beta}$-unsaturated-alkene-aldehyde;
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