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Effects of Body Weight and Dietary Protein Level on Ammonia Excretion by the Nile tilapia Oreochromis niloticus (나일틸라피아의 암모니아 배설에 미치는 어체중과 사료 내 단백질 함량의 영향)

  • Oh, Sung-Yong;Jo, Jae-Yoon
    • Journal of Aquaculture
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    • v.18 no.2
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    • pp.122-129
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    • 2005
  • Ammonia is the major limiting factor in intensive aquaculture production systems. Therefore, quantification of ammonia excretion is important for the water quality management in aquaculture systems. Ammonia excretion is known to be affected by many factors such as body weight and dietary protein level (DPL). In this study, experiments were carried out to investigate the effects of body weight and DPLs on the rates of ammonia excretion of Nile tilapia Oreochromis niloticus. Three sizes of fishes (mean initial weight; 4.8 g,42.7 g and 176.8 g) were fed each of two dietary protein levels (30.5% and 35.5%). Daily feeding levels for the three fish sizes of 4.8 g, 42.7 g and 176.8 g were 6%, 3%, and 1.5% body weight per day, respectively. Each group of fish was stocked in a 17.1-L aquarium and all treatments were triplicated. Following feeding, the weight-specific ammonia excretion rate of O. niloticus increased, peaked at 4 to 8 h, and returned to pre-feeding levels within 24 h. Total ammonia nitrogen (TAN) excretion.ate per unit weight decreased with the increase of fish weight for each diet (P<0.05). The TAN excretion rate increased with increasing dietary protein content for each fish size (P<0.05). TAN excretion rates (Y) for each diet with different fish weights were described by the following equations: low DPL diet (30.5%): $Y\;(mg\;kg^{-1}\;d^{-1})=955.69-147.12\;lnX\;(r^2=0.95)$, high DPL diet (35.5%): $Y\;(mg\;kg^{-1}\;d^{-1})=1362.41-209.79\;lnX\;(r^2=0.99)$. Where: X=body weight (g wet wt.). The TAN excretion rates ranged 28.5%-37.1% of the total nitrogen ingested for the low DPL diet (30.5%) and 37.4-38.5% for the high DPL diet (35.5%). Total nitrogen losses of fish fed the high DPL diet $(35.5%;\;0.26\sim0.91g\;kg^{-1}\;d^{-1})$ were higher than those fed the low DPL diet $(30.5%;\;0.22\sim0.68g\;kg^{-1}\;d^{-1})$. The losses decreased per kg of fish as fish size increased. Results will provide valuable information fer water quality management and culture of Nile tilapia in recirculating aquaculture systems.

Antioxidant and antiproliferating effects of Setaria italica, Panicum miliaceum and Sorghum bicolor extracts on prostate cancer cell lines (조, 기장, 수수 추출물의 항산화 효과 및 전립선 암세포주 증식 억제 효능)

  • Kim, Jeong-Ho;Cho, Hyun-Dong;Hong, Seong-Min;Lee, Ju-Hye;Lee, Yong-Seok;Kim, Du-Hyun;Seo, Kwon-Il
    • Food Science and Preservation
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    • v.23 no.7
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    • pp.1033-1041
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    • 2016
  • In this study, we evaluated antioxidant and antiproliferating effects of Setaria italica extract (SIE), Panicum miliaceum extract (PME) and Sorghum bicolor extract (SBE). Antioxidant effects of these extracts were determined by assessing DPPH radical scavenging activity, $ABTS^+$ radical scavenging activity, reducing power and superoxide dismutase (SOD)-like activity. From high concentrations ($1,000{\mu}g/mL$) of each extract at DPPH radical scavenging activities of SIE, PME and SBE were 10.5%, 5.5% and 86.8% respectively, $ABTS^+$ radical activities were 4.92%, 5.9% and 62.3% respectively, reducing powers (OD 700) were 0.15, 0.18 and 1.7 respectively, and SOD-like activities were 17.0%, 15.9% and 38.6% respectively. In addition, SBE significantly decreased the cell viability of androgen-sensitive lymph node metastasis type of prostate cancer (LNCaP) cells in a dose-dependent manner. Morphological study of SBE-treated LNCaP cells revealed distorted and shrunken cell masses. SBE-induced cell death was confirmed by observation of nuclear condensation and increased formation of apoptotic bodies. The antiproliferative effect of SBE seems to be associated with the antioxidant activity of its polyphenol content. The results of this study indicate that SBE can exert antioxidant and antiproliferative effects and may be as a useful food material.

Development of Analysis Method of Caffeine and Content Survey in Commercial Foods by HPLC (HPLC를 이용한 카페인의 분석법 개발 및 시판 식품중 함유량 조사)

  • Kim, Hee-Yun;Lee, Young-Ja;Hong, Ki-Hyoung;Lee, Chul-Won;Kim, Kil-Saeng;Ha, Sang-Chul
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1471-1476
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    • 1999
  • A simple and practical method for determination of caffeine in foods was developed. The analysis of caffeine was performed by reverse phase high performance liquid chromatography using a ${\mu}-Bondapak\;C_{18}$ column at isocratic condition with methanol-acetic acid-water(20 : 1 : 79) on UV detector at 280 nm. The clean-up and extraction of caffeine in samples were based on a simple pretreatment using a Sep-Pak $C_{18}$ cartridge. Recovery rates obtained with this method for cider, candy, cookie, milk, ice cream and persimmon leaf tea were 99.23%, 99.50%, 99.17%, 99.37%, 98.93% and 99.10% respectively. And the detection limit of caffeine was $0.1\;{\mu}g/mL$. With this method, the range of caffeine contents extracted from coffee, green tea, black tea, Oolong tea(tea bag), soft drinks, ice cream, milk and commercial confectionery were $3.38{\sim}37.50\;mg/g,\;16.30{\sim}26.10\;mg/g,\;10.80{\sim}16.65\;mg/g,\;11.25\;mg/g,\;0.06{\sim}0.11\;mg/g,\;0.04{\sim}0.44\;mg/g,\;0.04{\sim}0.39\;mg/g\;and\;0.10{\sim}1.80\;mg/g$, respectively. But caffeine was not detected in the other tea such as Acanthopanax sessiliflorum tea, Angelica gigas tea, Angelica tea, Arrow root tea, Duchu'ng tea, Dunggulle tea, Ganoerma lucidum tea, Ginger tea powder, Persimmon leaf tea, Ssanghwa tea and Cocoa mix powder.

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Technology and Home Economics Teachers' Perception of Participation in School Curriculum Organization and High School Credit System (기술·가정과 교사의 학교교육과정 편성 참여와 고교학점제에 대한 인식)

  • Park, Mi Jeong;Lim, Yunjin;Kwon, Yoojin;Lee, Kwangjae
    • Journal of Korean Home Economics Education Association
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    • v.32 no.1
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    • pp.15-34
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    • 2020
  • The purpose of this study was to examine the secondary school technology and home economics teachers' perception of the school curriculum organization and high school credit system. For this purpose, the questionnaire data of 345 secondary technology and home economics teachers nationwide were analyzed through descriptive statistics, t-test, and F-test with SPSS 24. The research results were as follows. First, technology and home economics teachers recognized that current schools lacked the time to organize technology and home economics curriculum (61.1%) and the number of teachers (53%). Most of them have participated (62.0%) and were very willing to participate in the school curriculum organization (4.47, 89.9%). Second, technology and home economics teachers were aware of the high school credit system more than the average (3.34), and more negative (52.8%) than positive (37.7%). As a positive influence, students recognized career choices (3.88) and deepened professional content in their major fields (3.81). On the other hand, the negative impact was the decrease in choice due to non-entry subjects (3.90) and the difficulty in moving teachers to school (3.57). Third, in order to stably respond to the introduction of high school credit system, technology and home economics teachers recognized the importance of coordinating career elective courses (4.51), developing and disseminating teaching and learning materials for elective courses (4.46), separating technology and home economics (4.45), and providing training on evaluation methods and applications (4.44). This study would be useful to provide the basic information and data for the future development of technology and home economics curriculum at the national level based on high school credit system.

Antioxidative and Antimicrobial Activities of Aruncus dioicus var. kamtschaticus Hara Extracts (눈개승마(Aruncus dioicus var. kamtschaticus Hara) 용매 추출물의 항산화 및 항균활성)

  • Kim, Mi-Seon;Kim, Kyoung-Hee;Jo, Ji-Eun;Choi, Jong-Jin;Kim, Young-Jin;Kim, Jong-Hwan;Jang, Soon-Ae;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.1
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    • pp.47-55
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    • 2011
  • The solvent extracts of Aruncus dioicus var. kamtschaticus Hara, which were extracted by using several solvents with different polarities, were performed to investigate the antioxidant activities, whitening effect and antimicrobial activity. The content of total polyphenol of fractions from Aruncus dioicus var. kamtschaticus Hara extract showed the highest value ($335.88{\pm}2.26$ mg/g GAE) on ethyl acetate fraction. The ethyl acetate and n-butanol fractions were 0.06 mg/mL and 0.25 mg/mL as $IC_{50}$ values on DPPH radical scavenging, and $99.16{\pm}0.09%$ and $89.29{\pm}0.64%$ on ABTS radical scavenging activity, respectively. Also, reducing power and FRAP value were significantly higher on ethyl acetate fraction. The SOD like activity showed $80.76{\pm}0.61%$ on ethyl acetate and $72.34{\pm}0.79%$ on n-butanol. Tyrosinase inhibition activities (at 5 mg/mL) were $59.08{\pm}0.98%$ on ethyl acetate fraction. The chloroform fraction showed the strongest antimicrobial activities against B. cereus (14 mm), B. subtilis (12.5 mm), S. aureus (10.8 mm), E. coli (20.7 mm) at 0.1 mg/disc and the inhibition zone diameter of ethyl acetate fraction was 17.2 mm against E. coli at 0.5 mg/disc. The minimum inhibitory concentrations (MIC) of chloroform fraction against B. cereus and E. coli were 50 and $25{\mu}g$/mL, respectively. From these results, it is suggested that ethyl acetate and n-butanol fractions of Aruncus dioicus var. kamtschaticus Hara could be used as functional material for food additive ingredient and chloroform fraction could be suitable for the development of a food preservative.

Dietary Fiber and β-Glucan Contents of Sparassis crispa Fruit Fermented with Lactobacillus brevis and Monascus pilosus (유산균 및 홍국균 발효 꽃송이버섯 추출물과 잔사의 식이섬유와 베타 글루칸의 함량)

  • Lim, Chang Wan;Kang, Kyoung Kyu;Yoo, Young-Bok;Kim, Byung Hee;Bae, Song-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.41 no.12
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    • pp.1740-1746
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    • 2012
  • Sparassis (S.) crispa is an edible mushroom abundant in dietary fiber and ${\beta}$-glucan. The aim of this study was to prepare extracts and residues of the fruit bodies of S. crispa fermented with Lactobacillus (L.) brevis and Monascus (M.) pilosus and to measure the remaining dietary fiber and ${\beta}$-glucan. Dried powder of S. crispa containing 64.4 g/100 g total dietary fiber (2.6 g/100 g soluble and 61.8 g/100 g insoluble dietary fibers) and 24.0 g/100 g ${\beta}$-glucan was used as the starting material for the extraction. Raw and fermented S. crispa were extracted with hot water and three kinds of aqueous ethanol (50, 70, and 90%, v/v), respectively. A hot water extract from S. crispa fermented with M. pilosus had greater soluble dietary fiber content (19.3 g/100 g) than that from raw S. crispa with 14.6 g/100 g soluble dietary fiber or that from L. brevis-fermented S. crispa with 8.2 g/100 g soluble dietary fiber. The yield of the extract was 16.6% of intial weight of dried S. crispa. After hot water extraction of S. crispa fermented with M. pilosus, residues containing 90.5 g/100 g total dietary fiber (1.3 g/100 g soluble and 89.2 g/100 g insoluble dietary fibers) were obtained, and the yield was 69.6% of intial weight of dried S. crispa. The residue (31.0 g/100 g) contained more ${\beta}$-glucan than raw S. crispa or M. pilosus-fermented S. crispa (24.4 g/100 g). The resulting hot water extract and residue from S. crispa fermented with M. pilosus would be suitable for use in preparing liquid and powdered health functional foods, respectively.

Variation of Anthocyanin Contents according to Collection Site and Maturity in Black Soybean (검정콩 수집지역과 성숙기에 따른 안토시아닌 함량 변이)

  • Yi, Eun-Seob;Lee, Yong-Sun;Kim, Hee-Dong;Kim, Yong-Ho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.4
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    • pp.376-381
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    • 2008
  • Anthocyanin contents of black soybean were analyzed for development of superior breeding lines with high anthocyanin contents. Total 292 genotypes of black soybean collected through the whole country were analyzed by HPLC in which C3G (Cyanidin-3-Glucoside), D3G (Delphinidin-3-glucoside), and Pt3G (Petunidin-3-Glucoside) were detected main anthocyanin pigments and each pigment contents were significantly different according to genotypes. C3G content showed the highest value in all materials and its variation was also wide, whereas D3G and Pt3G were not detected in 4 and 24 genotypes. Mean value of C3G, D3G, and Pt3G contents were $8.05{\pm}4.225$, $1.80{\pm}0.854$, and $1.15{\pm}0.781\;mg/g$, respectively. In case collected sites, genotypes collected in Chungnam region were higher the anthocyanin contents than other collections, which was $13.75{\pm}3.861\;mg/g$. It might be concluded that it takes more than 36 days for anthocyanin accumulation since beginning of seed-coat pigments formation, in that case it showed $13.09{\pm}4.190\;mg/g.$. Also total anthocyanin contents were present higher concentration in seed coat as maturation period was longer from flowering stage.

Validation of a Method and Evaluation of Antioxidant Activity for the Simultaneous Determination of Riboflavin and Coixol in Coix lacryma-jobi var. ma-yuen Stapf Sprouts (율무 새싹 추출물의 Riboflavin과 Coixol의 동시 분석법 검증 및 항산화 활성)

  • Lee, Ji Yeon;Park, Jung Yong;Park, Chun-Geon;Kim, Dong Hwi;Ji, Yun-Jeong;Choi, Su Ji;Oh, MyeongWon;Hwang, Hosop;Lee, Yunji;Jeong, Jintae;Lee, Jeong Hoon;Seo, Kyung Hye
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.64 no.4
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    • pp.452-458
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    • 2019
  • Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 ℃ under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC50], 68.9 ± 4.1 g/mL; and ABTS, IC50, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.

The Statistical Approach-based Intelligent Education Support System (통계적 접근법을 기초로 하는 지능형 교육 지원 시스템)

  • Chung, Jun-Hee
    • Journal of Intelligence and Information Systems
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    • v.18 no.1
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    • pp.109-123
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    • 2012
  • Many kinds of the education systems are provided to students. Many kinds of the contents like School subjects, license, job training education and so on are provided through many kinds of the media like text, image, video and so on. Students will apply the knowledge they learnt and will use it when they learn other things. In the existing education system, there have been many situations that the education system isn't really helpful to the students because too hard contents are transferred to them or because too easy contents are transferred to them and they learn the contents they already know again. To solve this phenomenon, a method that transfers the most proper lecture contents to the students is suggested in the thesis. Because the difficulty is relative, the contents A can be easier than the contents B to a group of the students and the contents B can be easier than the contents A to another group of the students. Therefore, it is not easy to measure the difficulty of the lecture contents. A method considering this phenomenon to transfer the proper lecture contents is suggested in the thesis. The whole lecture contents are divided into many lecture modules. The students solve the pattern recognition questions, a kind of the prior test questions, before studying the lecture contents and the system selects and provides the most proper lecture module among many lecture modules to the students according to the score about the questions. When the system selects the lecture module and transfer it to the student, the students' answer and the difficulty of the lecture modules are considered. In the existing education system, 1 kind of the content is transferred to various students. If the same lecture contents is transferred to various students, the contents will not be transferred efficiently. The system selects the proper contents using the students' pattern recognition answers. The pattern recognition question is a kind of the prior test question that is developed on the basis of the lecture module and used to recognize whether the student knows the contents of the lecture module. Because the difficulty of the lecture module reflects the all scores of the students' answers, whenever a student submits the answer, the difficulty is changed. The suggested system measures the relative knowledge of the students using the answers and designates the difficulty. The improvement of the suggested method is only applied when the order of the lecture contents has nothing to do with the progress of the lecture. If the contents of the unit 1 should be studied before studying the contents of the unit 2, the suggested method is not applied. The suggested method is introduced on the basis of the subject "English grammar", subjects that the order is not important, in the thesis. If the suggested method is applied properly to the education environment, the students who don't know enough basic knowledge will learn the basic contents well and prepare the basis to learn the harder lecture contents. The students who already know the lecture contents will not study those again and save more time to learn more various lecture contents. Many improvement effects like these and so on will be provided to the education environment. If the suggested method that is introduced on the basis of the subject "English grammar" is applied to the various education systems like primary education, secondary education, job education and so on, more improvement effects will be provided. The direction to realize these things is suggested in the thesis. The suggested method is realized with the MySQL database and Java, JSP program. It will be very good if the suggested method is researched developmentally and become helpful to the development of the Korea education.

Development of Convertor supporting Multi-languages for Mobile Network (무선전용 다중 언어의 번역을 지원하는 변환기의 구현)

  • Choe, Ji-Won;Kim, Gi-Cheon
    • The KIPS Transactions:PartC
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    • v.9C no.2
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    • pp.293-296
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    • 2002
  • UP Link is One of the commercial product which converts HTML to HDML convertor in order to show the internet www contents in the mobile environments. When UP browser accesses HTML pages, the agent in the UP Link controls the converter to change the HTML to the HDML, I-Mode, which is developed by NTT-Docomo of Japan, has many contents through the long and stable commercial service. Micro Explorer, which is developed by Stinger project, also has many additional function. In this paper, we designed and implemented WAP convertor which can accept C-HTML contents and mHTML contents. C-HTML format by I-Mode is a subset of HTML format, mHTML format by ME is similar to C-HTML, So the content provides can easily develop C-HTML contents compared with WAP and the other case. Since C-HTML, mHTML and WML are used under the mobile environment, the limited transmission capacity of one page is also similar. In order to make a match table. After that, we apply conversion algorithm on it. If we can not find matched element, we arrange some tags which only can be supported by WML to display in the best shape. By the result, we can convert over 90% contents.