KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Validation of a Method and Evaluation of Antioxidant Activity for the Simultaneous Determination of Riboflavin and Coixol in Coix lacryma-jobi var. ma-yuen Stapf Sprouts

율무 새싹 추출물의 Riboflavin과 Coixol의 동시 분석법 검증 및 항산화 활성

  • Lee, Ji Yeon (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Park, Jung Yong (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Park, Chun-Geon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Kim, Dong Hwi (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Ji, Yun-Jeong (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Choi, Su Ji (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Oh, MyeongWon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Hwang, Hosop (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Lee, Yunji (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Jeong, Jintae (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Lee, Jeong Hoon (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science) ;
  • Seo, Kyung Hye (Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science)
  • 이지연 (충북대학교 식품공학과 대학원) ;
  • 박정용 (충북대학교 식품공학과 대학원) ;
  • 박춘근 (농촌진흥청 인삼특작부) ;
  • 김동휘 (농촌진흥청 인삼특작부) ;
  • 지윤정 (농촌진흥청 인삼특작부) ;
  • 최수지 (농촌진흥청 인삼특작부) ;
  • 오명원 (농촌진흥청 인삼특작부) ;
  • 황호섭 (충북대학교 식품공학과 대학원) ;
  • 이윤지 (농촌진흥청 인삼특작부) ;
  • 정진태 (농촌진흥청 인삼특작부) ;
  • 이정훈 (농촌진흥청 인삼특작부) ;
  • 서경혜 (농촌진흥청 인삼특작부)
  • Received : 2019.08.10
  • Accepted : 2019.10.24
  • Published : 2019.12.31
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Abstract

Coix lacryma-jobi var. ma-yuen (Rom. Caill.) Stapf (CL), which contains riboflavin and coixol, has traditionally been used to treat cancer and arthritis. However, no method for the simultaneous determination of riboflavin and coixol in CL sprouts has been established. In this study, we established and validated a high-performance liquid chromatography-diode array detection (HPLC-DAD) method for the identification and quantification of two reference markers, riboflavin and coixol, in CL sprout extracts. CL sprouts (whole sprouts and leaves) were subjected to extraction with 70% ethanol at room temperature and at 80 ℃ under reflux conditions. The two extractions were validated with respect to specificity, accuracy, precision, and linearity. The content of the two reference markers was highest in leaves extracted under reflux conditions (riboflavin, 8.23 ± 0.32 mg/g; coixol, 5.95 ± 0.04 mg/g). We also investigated the antioxidant activity of the extracts via 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) scavenging assays. The results indicated that extracts obtained from sprouts under reflux conditions had the strongest antioxidative effects (DPPH half maximal inhibitory concentration [IC50], 68.9 ± 4.1 g/mL; and ABTS, IC50, 34.9 ± 0.1 g/mL). These results can serve as baseline data for the simultaneous determination of the two reference marker compounds, riboflavin and coixol, and development of functional food materials using CL sprouts.

율무 새싹 추출물의 riboflavin과 coixol의 원료표준화를 위한 동시분석 조건을 검증하고, 율무 새싹의 전초와 잎 추출물의 항산화 활성과 함량의 상관관계에 대해 조사하였다. 1. HPLC 분석을 위한 riboflavin과 coixol의 피크가 각각 분리되어 특이성을 확인하였고, 일내 및 일간 정확성은 모두 회수율이 100.28%에서 103.98% 사이이며 정밀성은 모두 1.93%이하를 나타내므로 본 실험의 분석조건은 높은 정확성과 정밀성을 가지고 있다. 2. Riboflavin과 coixol의 동시분석 조건의 검출한계(LOD) 및 정량한계(LOQ)를 조사한 결과 검출한계는 0.71 및 0.31 ㎍/mL, 정량한계는 2.71 및 0.94 ㎍/mL이므로 확립된 HPLC 분석조건은 riboflavin과 coixol의 동시분석하기에 무리가 없는 분석법으로 조사되었다. 3. 율무 새싹의 전초와 잎의 riboflavin과 coixol의 함량을 본 연구에서 얻어진 동시분석법을 적용하여 잎의 환류 추출물에서 가장 높은 함량이 나타났다. 4. 항산화 활성 측정 시, DPPH와 ABTS+ 소거능에서 모두 율무 새싹의 전초보다 잎에서, 상온 추출보다 환류 추출이 더 낮은 IC50값을 나타내었다. 율무 새싹 추출물의 riboflavin과 coixol 및 항산화 활성과의 상관관계를 실시한 결과, coixol이 증가할수록 DPPH와 ABTS+ 소거능의 IC50값이 유의적으로 감소하는 경향이 나타나므로 원료표준화 지표뿐만 아니라 기능성 지표로 활용이 가능할 것으로 판단되었다.

Keywords

References

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