• 한방안이비인후피부과학회지
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• 제19권2호
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• pp.59-70
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• 2006

Objective : This study was performed to investigate the Anti-Inflammatory Effects of WooBangHaeGiTang on the Allergic Contact dermatitis. Method : Contact hypersensitivity assay, video microscope, WBC count, RBU count, neutrophil ratio, lymphpcyte ratio and GOT & GPT were measurd. Result : 1. At Contact hypersensitivity Aasay, at 24 hours after Control group & Sample group showed appreciably more ear swelling than Normal group, at 48 hours after Control group showed more ear swelling than Normal group & Sample group, at 72 hours after Control group & Sample group showed more ear swelling than Normal group 2. Regarding the number of WBC, at 24 or 48 hours after There are no significant difference among Normal group, Control group. Sample group, at 72 hours after Sample group showed more WBC than Control group. 3. Regarding the number of RBC, at 24 hours after Control group showed more RBC than Normal group, at 48 hours after Control group & Sample group showed more RBC than Normal group. at 72 hours after Control group showed more RBC than Normal group. 4. Regarding the percentage of neutrophil in WBC, at 24 & 48 hours after Control group & Sample group showed higher percentage than Normal group. 5. Regarding the percentage of Lymphocyte in WBC, at 24 hours after Sample group showed lower percentage than Normal group, at 48 hours after Sample group & Control group showed lower percentage than Normal group. 6. Regarding the GOT & GPT in blood, the GOT & GPT are in Normal scale, so there are no Hepatotoxicity in Normal group, Control group, Sample group. Conclusions : According to above experiments, WooBangHaeGiTang have some anti allergy effects on allergic contact dermatitis.

• 대한한의학방제학회지
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• 제27권2호
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• pp.179-187
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• 2019

Objective : The purpose of this paper is to analyze the difference between Baekho-tang(白虎湯) and Baekhogainsam-tang (白虎加人蔘湯). Methods : We compared the effect of Baekho-tang on atopic dermatitis and the effect of Baekhogainsam-tang on allergic contact dermatitis. We focused on the level of thirst. Results : After the Baekho-tang treatment, the SCORAD of atopic dermatitis patient decreased $63.8{\rightarrow}4.3$ and VAS of pruritus on atopic dermatitis patient were improved $8{\rightarrow}2$ respectively. Meanwhile, after the Baekhogainsam-tang treatment, the VAS of pruritus on allergic contact dermatitis patient was improved $7{\rightarrow}2$ and the thirst of the patient improved. Conclusions : There is a difference between Baekho-tang and Baekhogainsam-tang at the level of thirst. Baekhogainsam-tang continues to thirst hard enough to find water.

• Biomolecules & Therapeutics
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• 제12권2호
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• pp.129-132
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• 2004

During the screening program to discover antiatopic agents from herbal formulas, we investigated the inhibitory effect of Hwangryunhaedoktang (HT) on passive cutaneous anaphylaxis and oxazolone-induced mouse ear dermatitis. HT significantly inhibited PCA reaction in mice at doses of 50 and 200 mg/kg with inhibitory activity of 31 and 53%, respectively. HT at concentration of 0.05 and 0.1% inhibited ear swelling by 23 and 46% at 16 days in oxazolone-induced mouse ear dermatitis. Both HT with anti without human intestinal microflora showed potent inhibitory activity on the ${\beta}$-hexosaminidase release induced by IgE. Based on these findings, HT may be a usable agent for skin disorder contact dermatitis.

• 한국자원식물학회지
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• 제24권4호
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• pp.430-437
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• 2011

본 연구에서는 DNCB에 의해 인위적으로 접촉피부염을 유발시킨 후 천궁추출물을 표피에 처리하여 피부표면, 피부두께, 혈장내 성분, 혈장내 IgE 농도 및 항산화효소을 조사하여 표피의 회복능에 미치는 효과를 조사하였다. 천궁추출물은 DNCB에 의해 유발된 알레르기성 접촉피부염에 도포 시에 환부의 피부염이 급격히 호전되었으며, 피부표면은 정상군에 비교될 정도로 각질이 없어진 깨끗하고 매끄러운 상태로 되었으며, 표피층의 두께도 점차 정상군과 같은 상태로 회복되고 있는 것으로 관찰되었다. 또한 알레르기성 접촉피부염이 유발되어 체내 지질함량이 급격히 증가된 경우 지질함량을 낮춰 정상수준으로 회복시키는 지질함량의 개선에 효과적으로 작용하고 있으며, 직접적으로 혈액 내 IgE 수준의 감소에 관여하여 피부의 과민반응 해소에 관여할 것으로 생각된다. SOD와 catalase의 활성은 천궁추출물의 처리에 의해 정상군 수준으로 낮아지는 경향을 보이고 있어 항산화 효소의 활성을 낮춰 정상수준으로 회복시켜주는 효과를 갖는 것으로 생각된다. 결론적으로 천궁 추출물은 알레르기성 접촉피부염의 회복에 매우 효과적으로 작용하는 천연 유용자원으로 이용할 수 있을 것으로 생각된다.

• 대한본초학회지
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• 제27권5호
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• pp.37-44
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• 2012

Objectives : The present study was undertaken to determine whether the ethanol extact of Citri Pericarpium (CP, Pericarp of Citrus unshiu Markovich, Rutaceae) is effective against atopic dermatitis according to it's storage period. Methods : To evaluate antiallergic effect of CP and OCP (Old Citri Pericarpium) evaluated in vivo their inhibitory effects against passive cutaenous anaphylaxis (PCA) reaction induced by IgE-antigen complex and scratching behaviors by compound 48/80. The Anti-atopic effects were measured by contact dermatitis, prurient animal model and PCA reaction. Contact dermatitis in mice as a model of the Type IV reaction caused by Oxazolone. Results : The results showed that anti-pruritus effects, analgesic effects of CP was depends on its hesperetin contents. And It also showed that keep longer in storage appeared to be higher in hesperetin contents. Both CP and OCP(Old CP) have a dose-dependent analgesic action in acetic acid induced writhing syndrome. OCP Potently inhibited PCA reaction in mice, although OCP weakly inhibited in long term contact dermatitis model in mice. Conclusions : These results suggest that the Proportional to the storage period, Citri Pericarpium possesses analgesic effects and anti-allergic effects.

• 대한한방소아과학회지
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• 제29권2호
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• pp.1-15
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• 2015

Objectives In the theory of Korean Medicine, Cicadae Periostracum (CP) has been used to treat skin diseases such as inflammatory dermatitis, tetanus and pruritus. CP can reduce heat and disperse wind. In prior studies, anti-allergic effect and anti-inflammatory effect of CP were reported. However, there has been no report regarding the correlation of CP and allergic contact dermatitis (ACD). This study was performed to show the effects of CP in ACD induced by 2,4-dinitrochlorobenzene (DNCB) in mice. Methods In this experiment, the effects of CP on biological changes were measured, such as changes in ear and spleen weight, ear and dorsum skin thickness, clinical aspect on the dorsum skin and histological changes. The effect of proliferation rate of splenocytes was also investigated in vitro and vivo study. Results In results, CP application (CPA) group and CP application and administration (CPAA) group were significantly restrained in ear weight gain and increase in ear and dorsum skin thickness compared to the control group. In addition, CPA and CPAA group showed diminished erythema, desquamation, bloodstain, and marks. Also, the histological assessment showed that CP treatement diminished thickness of epidermis, hyperkeratosis, pigmentation and parakeratosis. Conclusions In conclusion, these data suggest that CP can decrease symptoms of ACD.

• 농촌의학ㆍ지역보건
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• 제20권2호
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• pp.187-195
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• 1995

Hwamunsok(mat with flower design) is a special product of Kanghwa, which is made up of cyperus exaltatus. According to Allergy, cyperus exaltatus is one of the inhalant allergens and provokes respiratory symptoms and signs. There have been few reports about respiratory allergic disease or contact dermatitis occurring in farmers who cultivate the cyperus exaltatus or manufacture Hwamunsok with it. We studied the relationship between allergic symptoms and exposure to cyperus exaltatus. First, we questioned 141 voluntaries living in Kangwha on the symptoms of contact dermatitis, allergic rhinitis and bronchial asthma. Secondly, open tests with cyperus exaltatus were done to permitted 73 voluntaries. Patch tests with the European standard series(23 antigens) were only done to exposure 49 subjects to cyperus exaltatus. The results were as follows : 1. As the manifestation rate of allergic symptoms by age, its of 60 years old over in male showed 333 per 1,000 persons. Its of 50 years old in female showed 412 per 1,000 persons. Those age groups in both genders exhibited the highest manifestation of allergic symptoms than all other age groups. Also, if age and sex were adjusted with the rural population of Kanghwagun('93 base population), the manifestation rate of allergic symptoms in male showed 283 per 1,000 persons. Its in female and totals of study populations showed which is each 206 and 234 per 1,000 persons. Specially, it showed that statistics significantly(p=0.002), exposured subjects by cultivation of cyperus exaltatus & manufacture of Hwamunsok have experienced symptoms of contact dermatitis more frequently than unexposured subjects. Secondly, it tested statistics significantly that the risk factor as manifestation of allergic symptoms was the exposure of cyperus exaltatus's allergen(p=0.024). The manifestation risk of allergic symptoms in exposure groups was 3.73 times higher than unexposured subjects. Thirdly, positive reactions in open tests with cyperus exaltatus were higher in exposured subjects than non-exposured subjects. After 72 and 96 hours, positive reactions in open tests were all 10 subjects(20.4%). Also, after 1 week, positive reactions were 8 subjects(16.4%). In conclusion there is a close relationship between contact dermatitis and exposure to cyperus exaltatus while cultivating it and manufacturing Hwamunsok. Therefore, various studies should be continued and a health education program undertaken on contact dermatitis with those who were be engaging in Hwamunsok's manufacture.

• Journal of Preventive Medicine and Public Health
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• 제32권2호
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• pp.130-140
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• 1999

Objectives: This study was carried out to estimate the magnitude of skin disease related to occupation and to find out the characteristics of it. Methods: We collected and analyzed the cases of occupational skin disease reported by surveillance system composed of doctors and nurses in 150 enterprises with dispensary or attacked hospital and physicians in 92 specific health examination institutes and 150 dermatologists from May to November, 1998. Results: Among members of surveillance system, 66 enterprises and 47 specific health examination institutes and 55 dermatologists reported 571 cases of occupational skin disease in 512 workers. Excepting 81 cases reported by dermatologists, We analyzed 490 cases reported by enterprises and specific health examination institutes. Among 490 cases, contact dermatitis was most common(368 cases, 75.1%) and the second was hyper or hypopigmentation(36 cases, 7.3%). When we analyzed the characteristics of workers with occupational contact dermatitis, male workers were 281 (79.2%) and female were 74(20.8%). 165 workers(64.5%) had chronic skin disease with repeated cure and relapse. 245 workers(72.5%) answered positively that their coworkers had similar skin disease. 27 workers(8.7%) experienced absence due to contact dermatitis related to occupation. To analyze the type of industries of workers with occupational contact dermatitis, automobile and trailer manufacturing industry was most common(105 cases, 29.6%) and the second was manufacturing industry for image, sound and communication equipment(55 cases, 15.5%). Organic solvent(183 cases, 46.7%) was the most common treating material of workers with contact dermatitis and the second was various kinds of chemicals(59cases, 15.1%). Conclusions: This is the first study using nationwide surveillance system to collect data of occupational skin disease. We found that many workers had skin disease related to occupation and characteristics of occupational skin disease were chronic and clustering. Therefore, we had to establish counterplan to manage occupational skin disease and to operate surveillance system to identify trends of occupational skin disease, continuously.

• 해부∙생물인류학
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• 제31권4호
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• pp.143-149
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• 2018

접촉피부염은 피부가 이물질과 접촉함으로써 일어나는 염증반응으로 백혈구 혈관외유출이 매우 중요한 역할을 한다는 것은 잘 알려진 사실이다. 선행연구 결과 CD99가 ${\beta}1$ 인테그린 의존적 메커니즘을 통하여 단핵구의 혈관외유출을 조절하며, 따라서 염증 질환에 대한 치료제 개발의 신규 표적 분자일 가능성이 제시되었다. 본 연구에서는 CD99 유래 펩타이드인 CD99CRIII3가 단핵구의 혈관외유출과 접촉피부염 생쥐 모델에서 염증 반응을 억제하는지를 조사하였다. 인간 단핵구 세포주인 U937를 CD99CRIII로 처리할 경우 농도의존적으로 ${\beta}1$ integrin의 활성도가 감소되었으며, 이 세포주의 사람제대정맥내피세포에의 부착과 혈관외유출도 억제되었다. 나아가 CD99CRIII3는 포르볼 미리스테이트 아세테이트 처리에 의해 유발된 생쥐 접촉피부염 모델에서 Evans Blue의 혈관투과와 귀조직 무게를 농도 의존적으로 억제하였다. 이러한 결과들은 CD99CRIII3가 단핵구의 혈관외유출과 접촉피부염 동물 모델에서 일어나는 염증반응을 억제함을 보여준다. 이와 같이, 본 연구는 CD99 유래 펩타이드가 피부 염증질환에 대한 치료제로 개발될 가능성이 있음을 제시한다.

• 대한한방피부미용학회지
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• 제1권1호
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• pp.16-40
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• 2005

Objective : To study the effectiveness of Yangkyuksanhawtang against Allergic Contact Dermatitis I observed the change of cutaneous shape, histochemistry, immunohistochemistry, and the distribution of apoptotic cells. materials and methods : I divided 4-month-old rats into three groups of 10, which are a contrastive group of having applied Acetone olive oil only, ACD group to have intentionally activated Allergic Contact Dermatitis by DNCB and YST group to give medication of Yangkyuksanhawtang extract. And I observed each group of mice after 24, 48 and 72 hours. results : 1. With the result of Contact hypersensitivity assay, YST group shows appreciably less ear swelling than ACD group. 2. Comparing YST and ACD groups to each other regarding general change of skin, YST group shows less hyperplasia of epidermis, less migration of inflammatory cells and less damage of epidermis than ACD group. 3. Regarding the change of collagen fiber, ACD group has appeared to be low in number of collagen fiber while YST shows similarity with the contrastive group. 4. In dermis YST group has showed lower number of mastocyte than ACD group and is granulated type. 5. In dermis YST group has showed less MAC-1, IL-1 , $IL-2R-\;{\alpha}$ G, ICAM-1 and VCAM-1 than ACD group. 6. The distribution of apoptotic cells has appeared littler in YST group than in ACD 7. Among signal molecule of apoptosis Bcl-2 has distributed more in YST group than ACD group and Bax and Fas has distributed less in YST group than ACD group.