Effect of Hwangryunhaedoktang on Contact Hypersensitivity and Passive Cutaneous Hypersensitivity in Mice

  • Lee, Kun-Ho (College of Pharmacy, Kyung Hee University) ;
  • Shin, Young-Wook (East-West Pharmaceutical Research Institute, Kyung Hee University) ;
  • Kim, Dong-Hyung (College of Pharmacy and East-West Pharmaceutical Research Institute, Kyung Hee University)
  • Published : 2004.06.01

Abstract

During the screening program to discover antiatopic agents from herbal formulas, we investigated the inhibitory effect of Hwangryunhaedoktang (HT) on passive cutaneous anaphylaxis and oxazolone-induced mouse ear dermatitis. HT significantly inhibited PCA reaction in mice at doses of 50 and 200 mg/kg with inhibitory activity of 31 and 53%, respectively. HT at concentration of 0.05 and 0.1% inhibited ear swelling by 23 and 46% at 16 days in oxazolone-induced mouse ear dermatitis. Both HT with anti without human intestinal microflora showed potent inhibitory activity on the ${\beta}$-hexosaminidase release induced by IgE. Based on these findings, HT may be a usable agent for skin disorder contact dermatitis.

Keywords

References

  1. Austin, L. M., Ozawa, M., Kikuchi, T., Walters, I. B., Krueger, J. G. (1999) The majority of epidermal T cells in psoriasis vulgaris lesions can produce type 1 cytokines, interferon-r, interleukin-2, and tumor necrosis factor-a, defining Tcl (cytotoxic T lymphocyte) and Thl effector populations: a type 1 differentiation bias is also measured in circulating blood T cells in psoriatic patients. J. Invest. Dermatol., 113, 752-759 https://doi.org/10.1046/j.1523-1747.1999.00749.x
  2. Akagi, M., Mio, M., Tasaka, K. (1983) Histamine release inhibition and prevention of the decrease in membrane fluidity induced by certain antiallergic drugs: analysis of the inhibitory mechanism of NCO-650. Agents Actions, 13, 149-156 https://doi.org/10.1007/BF01967320
  3. Choi, O. H., Kim, J. H., Kinet, J. P. (1996) Calcium mobilization via sphingosine kinase in signaling by the Fc epsilon. RI antigen receptor Nature, 380, 634-636 https://doi.org/10.1038/380634a0
  4. Cox, J. S. G. (1967) Disodium cromglycate (FPL-670) ('lntar'): A specific inhibitor of reaginic antibody-antigen mechanism. Nature, 216, 1326-1329
  5. Friedman, E. S., LaNatra, N., Stiller, M. J. (2002) NSAIDs in dermatologic therapy: review and preview. J. Cutan. Med. Surg., 6, 449-59 https://doi.org/10.1007/s10227-001-0137-3
  6. Fujii, Y., Takeuchi, H., Tanaka, K., Sakuma, S., Ohkubo, Y., Mutoh, S. (2002) Effects of FK-506 (tacrolimus hydrate) on chronic oxazolone-induced dermatitis in rats. Eur. J. Pharmacol.,456, 115-121 https://doi.org/10.1016/S0014-2999(02)02554-2
  7. Hernandez, G. L., Volpert, O. V., Iniguez, M. A., Lorenzo, E. Martinez-Martinez, S., Grau, R., Fresno, M., Redondo, J.M. (2001) Selective inhibition of vascular endothelial growth factor-mediated angiogenesis by cyclosporin A: roles of the nuclear factor of activated T cells and cyclooxygenase 2. J Exp Med., 193, 607-20 https://doi.org/10.1084/jem.193.5.607
  8. Katayama, S., Shionoya, H., Ohtake, S. (1978) A new method for extraction of extravasated dye in the skin and the influence of fasting stress on passive cutaneous anaphylaxis in guinea pigs and rats. Microbiol. Immunol., 22, 89-101
  9. Kim, D. H. (2002) Herbal medicines are activated by intestinal microflora. Nat. Prod. Sci., 8, 35-43
  10. Kobashi, K., Akao, T. (1997) Relation of intestinal bacteria to pharmacological effects of glycosides. Bifidobacteria Microflora, 16, 21-7
  11. Nicoloff, B. J. (1991) The cytokine network in psoriasis. Arch. Dermatol., 127, 871-884
  12. Ovigne, J.-M., Baker, B. S., Brown, D. W., Powles, A. V., Fry, L. (2001) Epidermal CD8+ T cells in chronic plaque psoriasis are Tc1 cells producing heterogeneous levels of interferon-gamma. Exp. Dermatol., 10, 168-174
  13. Sakuma, S., Higashi, Y., Sato, N., Sasakawa, T., Sengoku, T., Ohkubo, Y., Amaya, T., Goto, T. (2001) Tacrolimus suppressed the production of cytokines involved in atopic dermatitis by direct stimulation of human PBMC system (Comparison with steroids). Int Immunopharmacol., 1,1219-1226 https://doi.org/10.1016/S1567-5769(01)00059-5
  14. Schafer-Korting, M., Schrrtid, M. H., Korting, H. C. (1996) Topical glucocorticoids with improved risk-benefit ratio. Drug Safety, 14, 375-385 https://doi.org/10.2165/00002018-199614060-00003
  15. Shibata, H., Mio, M., Tasaka, K. (1985) Analysis of the mechanism of histarrtine release induced by substance P. Biochim. Biophys. Acta, 846, 1-7
  16. Stevens, R. L., Austen, K. F. (1989) Recent advances in the cellular and molecular biology of mast cells. Immunol. Today, 10, 381-386
  17. Wuthrich, B. (1989) Epidemiology of the allergic diseases: are they really on the increase? Int. Arch. Allergy Appl. Immunol., 90(suppl. 1), 3-10