• Asian-Australasian Journal of Animal Sciences
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• v.33 no.6
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• pp.888-901
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• 2020

Objective: A set of microsatellite markers with high polymorphism from Tsaiya duck were used for the genetic monitoring and genetic structure analysis of Brown and White Tsaiya duck populations in Taiwan. Methods: The synthetic short tandem repeated probes were used to isolate new microsatellite markers from the genomic DNA of Tsaiya ducks. Eight populations, a total of 566 samples, sourced from Ilan Branch, Livestock Research Institute were genotyped through novel and known markers. The population genetic variables were calculated using optional programs in order to describe and monitor the genetic variability and the genetic structures of these Tsaiya duck populations. Results: In total 24 primer pairs, including 17 novel microsatellite loci from this study and seven previously known loci, were constructed for the detection of genetic variations in duck populations. The average values for the allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.29, 5.370, 0.591, 0.746, and 0.708, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting Brown Tsaiya duck cluster and a spreading White Tsaiya duck cluster. The Brown Tsaiya ducks and the White Tsaiya ducks with Pekin ducks were just split to six clusters and three clusters when K was set equal to 6 and 3 in the Bayesian cluster analysis. The individual phylogenetic tree revealed eight taxa, and each individual was assigned to its own population. Conclusion: According to our study, the 24 novel microsatellite markers exhibited a high capacity to analyze relationships of inter- and intra-population in those populations with a relatively limited degree of genetic diversity. We suggest that duck farms in Taiwan could use the new (novel) microsatellite set to monitor the genetic characteristics and structures of their Tsaiya duck populations at various intervals in order to ensure quality breeding and conservation strategies.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.167-179
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• 2018

Objective: The aim of this study was to create a set of microsatellite markers with high polymorphism for the genetic monitoring and genetic structure analysis of local goose populations. Methods: Novel microsatellite markers were isolated from the genomic DNA of white Roman geese using short tandem repeated probes. The DNA segments, including short tandem repeats, were tested for their variability among four populations of geese from the Changhua Animal Propagation Station (CAPS). The selected microsatellite markers could then be used to monitor genetic variability and study the genetic structures of geese from local geese farms. Results: 14 novel microsatellite loci were isolated. In addition to seven known loci, two multiplex sets were constructed for the detection of genetic variations in geese populations. The average of allele number, the effective number of alleles, the observed heterozygosity, the expected heterozygosity, and the polymorphism information content were 11.09, 5.145, 0.499, 0.745, and 0.705, respectively. The results of analysis of molecular variance and principal component analysis indicated a contracting white Roman cluster and a spreading Chinese cluster. In white Roman populations, the CAPS populations were depleted to roughly two clusters when K was set equal to 6 in the Bayesian cluster analysis. The founders of private farm populations had a similar genetic structure. Among the Chinese geese populations, the CAPS populations and private populations represented different clads of the phylogenetic tree and individuals from the private populations had uneven genetic characteristics according to various analyses. Conclusion: Based on this study's analyses, we suggest that the CAPS should institute a proper breeding strategy for white Roman geese to avoid further clustering. In addition, for preservation and stable quality, the Chinese geese in the CAPS and the aforementioned proper breeding scheme should be introduced to geese breeders.

• Journal of Korean Society of Forest Science
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• v.94 no.3 s.160
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• pp.161-167
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• 2005

Above and belowground biomass for Phyllostachys pubescens was determined in Jinju, Gyeongsangnam-do. Regression analyses of dry weights of culms, foliages, and twigs versus diameter at breast height were used to calculate regression equations of the form of log Y = a + blogX. Total aboveground biomass for Phyllostachys pubescens was 69.7 ton/ha and rhizomes and roots biomass were 13.7 ton/ha and 7.5 ton/ha, respectively. Culms account for about 60% of total aboveground biomass. The aboveground biomass of each component was decreased in the order of culms>foliages>twigs. As diameter at breast height grew thicker, the proportion of culms to total aboveground biomass increased. The proportion of dry weight of culms to green weight gradually increased with height in a bamboo tree and ages. Nutrients (kg/ha) of litter layer were distributed as follows: N(45.1), Ca(17.3), K(6.1), Mg(3.6), P(3.5) and Na(0.7). Nitrogen and K were given much weight in total nutrients of rhizomes and roots. These results will be useful in measuring carbon stock and drawing up management plan to increase it for Phyllostachys pubescens stand.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.11
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• pp.1732-1740
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• 2020

Objective: The objectives of this study were to investigate the genetic diversity, population structure and relatedness among the five chicken populations of Bangladesh using microsatellite markers. Methods: A total of 161 individuals representing 5 chicken populations (non-descript Deshi [ND], naked neck [NN], hilly [HI], Aseel [AS], and red jungle fowl [JF]) were included in this study to investigate genetic diversity measures, population structure, genetic distance and phylogenetic relationships. Genotyping was performed using 16 selected polymorphic microsatellite markers distributed across 10 chromosomes. Results: The average observed and expected heterozygosity, mean number of alleles and polymorphic information content were found to be 0.67±0.01, 0.70±0.01, 10.7 and 0.748, respectively in the studied populations. The estimated overall fixation index across the loci (F), heterozygote deficiency within (F_IS) and among (F_IT) chicken populations were 0.04±0.02, 0.05 and 0.16, respectively. Analysis of molecular variance analysis revealed 88.07% of the total genetic diversity was accounted for within population variation and the rest 11.93% was incurred with population differentiation (F_ST). The highest pairwise genetic distance (0.154) was found between ND and AS while the lowest distance was between JF and AS (0.084). Structure analysis depicted that the studied samples can be categorized into four distinct types or varieties (ΔK = 3.74) such as ND, NN, and HI where AS and JF clustered together as an admixed population. The Neighbor-Joining phylogenetic tree and discriminant analysis of principal component also showed close relatedness among three chicken varieties namely AS, HI, and JF. Conclusion: The results reflected that indigenous chicken of Bangladesh still possess rich genetic diversity but weak differentiation among the studied populations. This finding provides some important insight on genetic diversity measures that could support the designing and implementing of future breeding plans for indigenous chickens of Bangladesh.

• Journal of Korean Society of Forest Science
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• v.37 no.1
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• pp.35-40
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• 1978

The uptake ratio of supplying $P^{32}$ labelled double superphosphate and the hastening efficiency of the uptake by addition of magnesium sulfate to the fertilizer were studied on the ${\times}Populus $ albaglandulosa planted with 0/1 cutting in 1975. The results are summerized as follows. 1. Average 13% of supplying double superphosphate was absorbed into ${\times}Populus$ albaglandulosa planted on the reddish heavy clay soil in Institute of Forest Genetics. 2. The accumulation of absorbed magnesium was more amount in leaf than in stem. 3. The uptake ratio of supplying double superphosphate was able to increase up to 16%~33% by the addition of magnesium sulfate to the fertilizer. 4. It might be possible to increase the tree growth following the acceleration of photosynthesis due to the increasing amount of magnesium known to be a component of chlorophyll in leaf as well as to hasten the efficiency of uptake of phosphorus by the addition of magnesium to double superphosphate.

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.343-359
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• 2013

In order to understand shoreline environment characteristics of Korean reservoirs, the interrelationships between environmental factors of geomorphology, hydrology, water quality and shoreline soil were analyzed, and the reservoir types were classified according to their environmental characteristics in the 35 reservoirs selected by considering the purpose of dam operations and annual water-level fluctuations. Geomorphological and hydrological characteristics of reservoirs were correlated with the altitude and the size scale of reservoirs. The annual range of water level fluctuation showed a wide variation from 1 m to 27 m in the various reservoirs in Korea. The levels of eutrophication of most reservoirs were mesotrophic or eutrophic. From the result of the soil texture analysis, sand contents were high in reservoir shorelines. Range, frequency and duration of water-level fluctuation were distinctive from the primary function of reservoirs. Flood control reservoirs had a wide range with low frequency and waterpower generation reservoirs had a narrow range with high frequency in the water-level fluctuation. According to the result of CART (classification and regression tree) analysis, the water quality of reservoirs was classified by water depth, range of water-level fluctuation and altitude. The result of PCA (principal component analysis) showed that the type of reservoirs was classified by reservoir size, water-level fluctuation, water quality, soil texture and soil organic matter. In conclusion, reservoir size, the water-level fluctuation, water quality and soil characteristics might be major factors in the environment of reservoir shorelines in Korea.

• Korean Journal of Environment and Ecology
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• v.18 no.1
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• pp.92-102
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• 2004

In this study, landscape changes were examined by periods through the analysis of visual quantity on the landscape components of national road including the mountains, the sky, road pavements, street trees, paddy fields and dry fields, billboard, telegraph pole, building, structure, slope, guard fence, soundproof wall, and median strip etc. For this, subject national road in suburb arreas was selected to grasp its landscape characteristics, and divisional criteria of landscape type of the national road were suggested to look into the trend of landscape changes by periods. Findings of this study are as follows：1.Divisional criteria of landscape type of the national road include location and geometry, structure, land use, perspective, and surrounding buildings of the national road. 2.The highest visual quantity was found in road pavements by periods among landscape components of the national road. It was found that this result has a thread of connection with previous study emphasizing the importance of road geometry as one of landscape components, and road geometry shall be regarded as a visually significant component in analyzing and evaluating landscape of the national road. 3.Most distinguished factors of landscape changes of the national road by periods were median strip(F＝33.296) and street tree(F＝32.881), and then the sky(F＝24.735) and mountain(F＝23.477) showed similar level of statistically significant difference. However, statistically significance difference was not found in buildings and structures. 4.It was shown that natural elements decreased gradually but structural landscape made of artificial elements became the main in construction of the national road. This result implies that although the scenic and ecological soundness is recognized in construction of the national road, it is not out of the limitation of functionalities that is, access and mobility.

• Journal of Korean Society of Forest Science
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• v.102 no.2
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• pp.239-246
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• 2013

This study observed changes of understory vegetation to evaluate the role of forest aggregate after 15% aggregated retention harvest in mature oak forest (> 100 years) in Gyungsangbukdo Bonghwagun in 2010 and 2011. Spontaneous responses of understory vegetation cover (%), species richness, abundance of plant growth forms (herbaceous and woody plants), and overall attributes (by Ordination analysis) were estimated in aggregate area (0.15 and n=36) and clear cut area (0.85 and n=192) in experimental site and control site (1 and n=300). Based on ordination analysis, overall change of species composition in aggregated sites were relatively lower than in harvest area. Right after treatment, total cover of cutted area slightly decreased from 15.6% to 14.7%, and species richness increased from 14 species to 22 species. Cover and richness in the both of aggregate and control sites increased. In plant growth forms, 15% aggregate harvest revealed positive effects on the abundance (cover and richness) of herbaceous plants than woody group. After retention treatment, overall, edge effect likely played major component of vegetation changes in aggregate forest and in harvested area, mechanical damage from harvest operation and change of forest structure by clear cutting were critical. As pre-treatment data, which are rare in ecological studies in Korea, were critical for interpretation between patterns that may have arisen from spatial distributions in the original forest, our experimental design have higher opportunity for long term monitoring on the effect of forest aggregate and vegetation regeneration in clear cutted area.

• Journal of Korean Society of Forest Science
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• v.88 no.1
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• pp.93-100
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• 1999

This study has been carried out to estimate aboveground and soil carbon contents in an average 39-year-old Quercus mongolica and 40-year-old Quercus variabilis stands in Chungju, Chungbuk. Ten sample trees were cut in each forest and soil samples were collected. Aboveground carbon content was estimated by the equation model $Wt=aD^b$ where Wt is oven-dry weight in kg and D is DBH in cm. Total aboveground carbon content was 48.85tonC/ha in Quercus mongolica stand and 57.49tonC/ha in Quercus variabilis stand. The proportion of each tree component to total aboveground carbon content was high in order of bolewood, branches, bolebark, and leaves in the two forests. Aboveground net primary production was estimated at 5.88tonC/ha in Quercus mongolica stand and 5.12tonC/ha in Quercus variabilis stand. Soil carbon content was 67.0tonC/ha in Quercus mongolica stand, 67.8tonC/ha in Quercus variabilis stand, and 54.7tonC/ha in Pinus densiflora stand. There was no significant difference in soil carbon content among the three forests.

• Korean journal of applied entomology
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• v.44 no.3 s.140
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• pp.189-197
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• 2005

Cellulose-digesting bacteria were isolated from hindgut of Allomyrina dichotoma (Coleoptera: Dynastidae). The bacterial isolates were identified as Yersinia sp. and Bacillus sp. The addition of the identified bacteria to diet increased growth rate of the cetoniid beetle, Protaetia brevitarsis senlensis (Coleoptera: Cetoniidae), probably by digesting cellulose nutrient contained in the oak tree sawdust diet. An additive of wheat flour at more than 10% to the sawdust diet significantly enhanced growth of P. brevitarsis senlensis. Trimmed branches of apple trees have been disposed in the apple farms and could be used for a diet component of the cetoniid beetle when the cellulose-digesting bacteria were mixed with the derived-sawdust. Resulting manure from mass rearing of P. brevitarsis senlensis contained high organic matters and trace amounts of toxic metals. When the manure were splayed on soil, it was effective as a natural compost and significantly stimulated lettuce growth. This research suggests a model technology to use cellulose-digesting bacteria to use for culturing grub, which results in natural recycles of trimmed branches in apple farms as grub diet, and to use grub manure as a natural compost.