Browse > Article

A Grub (Protaetia brevitarsis seulensis) Rearing Technique Using Cellulose-digesting Bacteria and Natural Recycling of Rearing Byproduct to an Organic Fertilizer  

Kang, Sang-Jin (Department of Agricultural Biology, Andong National University)
Park, Chun-Woo (Green Agrotech Inc.)
Han, Sang-Chan (Department of Agricultural Biology, Andong National University)
Yi, Young-Keun (Department of Agricultural Biology, Andong National University)
Kim, Yong-Gyun (Department of Agricultural Biology, Andong National University)
Publication Information
Korean journal of applied entomology / v.44, no.3, 2005 , pp. 189-197 More about this Journal
Abstract
Cellulose-digesting bacteria were isolated from hindgut of Allomyrina dichotoma (Coleoptera: Dynastidae). The bacterial isolates were identified as Yersinia sp. and Bacillus sp. The addition of the identified bacteria to diet increased growth rate of the cetoniid beetle, Protaetia brevitarsis senlensis (Coleoptera: Cetoniidae), probably by digesting cellulose nutrient contained in the oak tree sawdust diet. An additive of wheat flour at more than 10% to the sawdust diet significantly enhanced growth of P. brevitarsis senlensis. Trimmed branches of apple trees have been disposed in the apple farms and could be used for a diet component of the cetoniid beetle when the cellulose-digesting bacteria were mixed with the derived-sawdust. Resulting manure from mass rearing of P. brevitarsis senlensis contained high organic matters and trace amounts of toxic metals. When the manure were splayed on soil, it was effective as a natural compost and significantly stimulated lettuce growth. This research suggests a model technology to use cellulose-digesting bacteria to use for culturing grub, which results in natural recycles of trimmed branches in apple farms as grub diet, and to use grub manure as a natural compost.
Keywords
Allomyrina dichotoma; Bacillus; Cellulose; Compost; Gut bacteria; Natural recycling; Protaetia brevitarsis senlensis; Yersinia;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Brauman, A., D.E. Bignell and I. Tayasu. 2000. Soil-feeding termites: biology, microbial associations and digestive mechanisms. pp. 233-259. In Termites: evolution, sociality, symbiosis, ecology, eds. T. Abe, D.E. Bignell and M. Higashi. Kluwer Academic Publishers, Dordrecht, The Netherlands
2 Egert, M., B. Wagner, T. Lemke, A. Brune and M.W. Friedrich. 2003. Microbial community structure in midgut and hindgut of the humus-feeding larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae). Appl. Environ. Microbiol. 69: 6659-6668   DOI   ScienceOn
3 Holt, J.G., N.R. Krieg, P.A. Sneath, J.T. Stanley and S.T. Williams. 1994. Bergey's manual of determinative bacteriology. Williams & Wilkins, Baltimore
4 Lee, S.C. 1997. Agricultural development strategy in Bong-Wha country. Korean J. Agri. Ext. 4: 195-199
5 Brauman, A. 2000. Effect of gut transit and mound deposit on soil organic matter transformations in the soil feeding termite: a review. Eur. J. Soil Biol. 36: 117-125   DOI   ScienceOn
6 Breznak, J.A. and A. Brune. 1994. Role of microorganisms in the digestion of lignocellulose by termites. Annu. Rev. Entomol. 39: 453-487   DOI   ScienceOn
7 Lavelle, P., D. Bignell, M. Lepage, V. Wolters, P. Roger, P. Ineson, O.W. Heal. 1997. Soil function in a changing world: the role of invertebrate ecosystem engineers. Eur. J. Soil Biol. 33: 159-193
8 Kim, Y.K. and S.Y. Ahn. 1996. The effect of cellulase treatment on the physiochemical properties of rice and the texture of cooked rice. Korean J. Food Sci. Tech. 28: 720-729
9 Cazemier, A.E., J.C. Verdoes, F.A.G. Reubsaet, J.H.P. Hackstein, C. van der Drift and J.M. Op den Camp. 2003. Promicromonospora pachnodae sp. nov., a member of the (hemi)cellulolytic hindgut flora of larvae of the scarab beetle Pachnoda marginata. Antonie van Leeuwenhoek 83: 135-148   DOI   ScienceOn
10 Grayson, J.M. 1958. Digestive tract pH of six species of Coleoptera. Ann. Entomol. Soc. Am. 51: 403-405   DOI
11 Choi, J.S., J.K. Hwang, C.T. Kim and D.S. Dong. 1996. Enzymatic solubilization of thermally treated Jujube tissues. Food Ind. Nut. 1: 49-79
12 Lemke, T., U. Stingl, M. Egert, M.W. Friedrich and A. Brune. 2003. Physicochemical conditions and microbial activities in the highly alkaline gilt of the humus-feeding larva of Pachnoda ephippiata (Coleoptera: Scarabaeidae). Appl. Environ. Microbiol. 69: 6650-6658   DOI   ScienceOn
13 Brune, A. 1998. Termite guts: the world's smallest bioreactors. Trends Biotechnol. 16: 16-21   DOI   ScienceOn
14 Lee, Y.K. 2000. Laboratory manual for plant bacterial pathology. 110 pp. Andong National University. Agricultural Science and Technology Institute, Andong
15 Lo, N., H. Watanabe and M. Sugimura. 2003. Evidence for the presence of a cellulase gene in the last common ancestor of bilaterian animals. Proc. R. Soc. Lond. B 270: S69-S72
16 SAS Institute, 1988. SAS/STAT user's guide, Release 6.03, Ed. Cary, N.C
17 Bayon, C. and J. Mathelin. 1980. Carbohydrate fermentation and by-product absorption studied with labelled cellulose in Oryctes nasicornis larvae (Coleoptera: Scarabaeidae). J. Insect Physiol. 26: 819-828   DOI   ScienceOn
18 Hong, S.P. and D.S. Kim. 1998. Chitosanolytic characteristics of cellulase from Trichoderma viride and Trichoderma reesei. Korean J. Food Sci. Tech. 30: 245-252
19 Wolters, V. 2000. Invertebrate control of soil organic matterstability. Biol. Fertil. Soils 31: 1-19   DOI   ScienceOn
20 Park, Y., K. Kim and Y. Kim. 2002. A pathogenic bacterium, Enterococcus faecalis, to the beet armyworm, Spodoptera exigua. J. Asia-Pacific Entomol. 5: 221-225   DOI   ScienceOn