• Bulletin of the Korean Chemical Society
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• v.32 no.4
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• pp.1183-1186
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• 2011

The unique Br signature was utilized for C-terminal amino acid sequencing of model peptides. C-terminal carboxyl group was selectively derivatized in peptides, containing side chain carboxyl group, using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) and Br was introduced using 4-bromophenylhydrazine hydrochloride (BPH) in a one pot reaction. Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) tandem mass spectra were obtained carrying the Br signature in the y-series ions. The Br signature facilitated C-terminal sequencing and discrimination of C-terminal carboxyl groups in the free acid and amide forms.

• Korean Journal of Environmental Biology
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• v.35 no.4
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• pp.526-532
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• 2017

Development, survival, and reproduction of brown planthopper (BPH) Nilaparvata lugens $St{\aa}l$ (Hemiptera: Delphacidae), were studied in laboratory at $25{\pm}2^{\circ}C$, $65{\pm}5%$ RH and a 16L : 8D hours photoperiodism on five rice cultivars of: Dongjin 1ho, Chungchungbyeo, Jangseongbyeo, Chinnongbyeo and Jungmo 1045. BPH nymphs successfully survived on all rice cultivars, although survival rate was lowest on Jangseongbyeo (36.0%). Developmental time of immature stages ranged from $11.7{\pm}0.59d$ on Jungmo 1045 to $12.8{\pm}0.59d$ on Chinnongbyeo. Reproductive period and female longevity were longest on Dongjin 1ho, Chinnongbyeo and Jungmo 1045 while highest fecundity of N. lugens being observed on these three rice cultivars. Highest and lowest net reproductive rates were calculated on rice cultivars, Jungmo 1045 and Jangseongbyeo, respectively. Mean generation time was the longest on rice cultivar Dongjin 1ho. Respective descending order of intrinsic rates of population increase were on Jungmo 1045, Chinnongbyeo, Dongjin 1ho, Chungchungbyeo and Jangseongbyeo. These population parameters showed that N. lugens can successfully survive and reproduce on Chinnongbyeo and Jungmo 1045.

• Korean journal of applied entomology
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• v.29 no.4
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• pp.217-221
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• 1990

Some biological phenomena of Anagrus incarnatus Haliday, an egg parasitoid of planthopper, were studied under three different constant temperatures. Duration from egg to adult emergence of the parasitoid from the BPH eggs were 21.5, 13.6 and 10.6 days under $20^{\circ}C$, $25^{\circ}C$ and $30^{\circ}C$, respectively. Rate of the development was highly correlated with temperature. The critical temperature was estimated as $10.0^{\circ}C$ and the effective degree days was 210.3 day-degree. Durations for the development of A. incarnatus under 25$\pm$$1^{\circ}C$ (16L : 8D) were 12.4, 12.5 and 12.1 days from eggs of N. lugens, S. furcifera and L. striatellus, respectively. Duration of the development of A. incarnatus from 1, 3, 5, 7 days old BPH eggs were 12.5, 12.1, 12.9 days, respectively. The average longevity of adult was 5.3 days under 25$\pm$$1^{\circ}C$. Number of the ovarian and practically oviposited eggs were 34.8$\pm$28 and 28.3$\pm$0., respectively. Female A. incarnatus laid most of the eggs within few days after the emergence ; over 60% within 24 hours, nearly 90% upto the 2nd day, and nearly 100% upto the 3rd day.

• Korean Journal of Microbiology
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• v.30 no.1
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• pp.8-14
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• 1992

Nucleotide Sequence of phnE Gene Encoding Extradiol Dioxygenase fromPseudomonas sp. Strain DJ77Kim, Young-Chang'.", Myeong-Su Shin1, Kil-Sang Younl, Young-Soon Park1, andUg-Hyeon Kim'.' (Department of Microbiology, C'hungbuk National University.Cheongju 360-763, KOREA. and 'Research Center for Molecular Microbiology,Seoul National University)nal University)

• Korean journal of applied entomology
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• v.41 no.2
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• pp.91-97
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• 2002

This study was conducted to determine the effects of temperature and food onthe nymphal development and adult longevity of the green virid bug, Cyrtorhinus lividipennis Router. Nymphal period was 13.2 to 13.7 days at 24$^{\circ}C$, 10.4 to 10.9 days at 28$^{\circ}C$, and 9.0 to 9.3 days at 32$^{\circ}C$. Number of eggs predated by nymph was 10.6-14.3 at 24$^{\circ}C$, 7.5-9.2 at 28$^{\circ}C$, and 5.8-7.5 at 32$^{\circ}C$. Amount of predation was highest on BPH, followed by SBPH, WBPH, and GLH. Number of nympal moulting was mostly four times (55-75%) followed by three times (25-45%), regardless of temperature and food. Percent emergence rate was 52-70%, 48-66% and 22-38% at 24$^{\circ}C$, 28$^{\circ}C$ and 32$^{\circ}C$, respectively, showing a tendency to decrease with increasing temperature, and was affected by foods. Adult longevity, when supplied with one food, was 19.8 to 22.5 days at 24$^{\circ}C$, 15.6 to 17.0 days at 28$^{\circ}C$, and 10.3 to 11.7 days at 32$^{\circ}C$. There was no significant difference among foods in terms of adult longevity, but it tended to be slightly longer when provided with four foods than with one food. Number of eggs predated by an adult, when supplied with one food, was 35.7-54.2, 31.6-44.6 and 18.1-28.2 at 24$^{\circ}C$, 28$^{\circ}C$ and 32$^{\circ}C$, respectively. It was highest on BPH, followed by SBPH, WBPH and GLH regardless of temperature. Number of egg Predated by an adult, when supplied with four foods, was 4.0-28.9, 2.9-29.0 and 17-13.6 at 24$^{\circ}C$, 28$^{\circ}C$ and 32$^{\circ}C$, respectively, and was significantly different among foods supplied. The results suggest that the significantly different predation amount by adult was due to food preference amoung the different foods.

• Proceedings of the Korean Society of Crop Science Conference
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• 2005.10b
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• pp.244-245
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• 2005

• Journal of Life Science
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• v.13 no.5
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• pp.569-575
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• 2003

Supercritical $CO_2$ extraction was applied to extraction of the valuable compound for benign prostatic hyperplasia from saw palmitto. Three levels of parameters, temperature $(35^{\circ}C,\; 60^<\circ}C)$, pressure 22∼30 Mpa and ethanol modified solvent concentration, were used as the independent variables of central composite to rotate the design. The extracting pressure and temperature interaction was significantly affected the extraction of valuable compounds. The ethanol was an efficient modifier to extract valuable compound by supercritical carbon dioxide. The optimal processing conditions were extracting pressure, 30 Mpa, extracting temperature, $60^<\circ}C$, ethanol modifier, 15% for extracts of the valuable compounds from saw palmitto. In conclusion, it was found that supercritical $CO_2$ extraction can successfully be applied to extract high quality extracts from saw palmitto.

• Microbiology and Biotechnology Letters
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• v.22 no.1
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• pp.37-44
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• 1994

The homology among the genes coding for degradation of bipheny(BP) and 4-chlorobiphenyl(4CB) was comparatively analyzed by Southern hybridization in several BP/4CB degrading bacterial strains. As the hybridization results of their genomic DNAs with pcbABCD as the DNA probe, the group of Pseudomonas sp. DJ-12. P08 and P27 strain was separated by the group of P20 and P1242 strains. The P. pseudoalcaligenes KF707 showed the hybidization signal which was homologous to the group of DJ-12, but they had different restriction endonuclease sites. The pcbAB genes in pCUl recombinant plasmid from Pseudomonas sp. DJ-12 appeared to be homologous to pchAB genes in pKTF20 cloned from P. pseudoalcaligenes KF707, but the C genes in both strains were not homologous. The bphABC in pKTF20 showed the signals homologous to the cbp ACB in pAW6194 cloned from P. putida OU83, but homologous signal was not found botween the pcbABCD genes in pCUl and the cbpADCB genes in pAW6194 recombbinant plasmid.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.275-275
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• 2002

Pseudomonas sp. S-47 is capable of catabolizing 4-chlorobenzoate (4CBA) as carbon and energy sources under aerobic conditions via the mesa-cleavage pathway. 4CBA-dioxygenase and 4CBA-dihydrodiol dehydrogenase (4CBA-DD) catalyzed the degradation af 4CBA to produce 4-chlorocatechol in the pathway. In this study, the xylL gene encoding 4CBA-DD was cloned from the chromosomal DNA of Pseudomonas sp. S-47 and its nucleotide sequence was analyzed. The xylL gene was found to be composed of 777 nucleotide pairs and to encode a polypeptide of 28 kDa with 258 amino acid residues. The deduced amino acid sequence of the dehydrogenase (XylL) from strain S-47 exhibited 98% and 60% homologies with these of the corresponding enzymes, Pseudomonas putida mt-2 (XyIL) and Acinetobacter calcoaceticus (BenD), respectively. However, the amino arid sequences show 30% or less homology with those of Pseudomonas putida (BnzE), Pseudomonas putida Fl (TodD), Pseudomonas pseudoalcaligenes KF707 (BphB), and Pseudomonas sp. C18 (NahB). Therefore, the 4CBA-dihydrodiol dehdrogenase of strain S-47 belongs to the group I dehydrogenase involved in the degradation of mono-aryls with a carboxyl group.

• The Korean Journal of Pesticide Science
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• v.2 no.2
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• pp.75-82
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• 1998

This study was conducted to find out the biochemical or metabolic resistance mechanism of brown planthopper (BPH) to carbofuran. Differences between resistant ($LD_{50};\;20.3{\mu}g/g$) and susceptible strains($LD_{50};\;0.3{\mu}g/g$) were shown. The amounts of carbofuran metabolite, benzofuranol, and the origin, not developed by Thin Layer Chromatography, were much more in the susceptible strain. But the mother compound, carbofuran, was much more in the resistant strain. The tendencies of metabolism one and three hours after treatment were similar in both strains except for the amounts of metabolites described above. From the study, it is supposed that hydrolytic enzyme, esterase, changes its role from cleaving the esteric bond of carbofuran to making conjugates with carbofuran. This seems to be the main resistance mechanism of BPH to carbofuran. Oxidase and transferase may play little or no role in resistance mechanism. Oxidative and transferring enzymes gave no effects on the metabolism of carbofuran in the resistant strain compared with the susceptible strain.