Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Homology Analysis Among the Biphenyl and 4-Chlorobiphenyl Degrading Genes by Southern Hybridization

Southern Hybridization에 의한 Biphenyl 및 4-Chlorobiphenyl 분해유전자들의 상동성 분석

  • Published : 1994.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

The homology among the genes coding for degradation of bipheny(BP) and 4-chlorobiphenyl(4CB) was comparatively analyzed by Southern hybridization in several BP/4CB degrading bacterial strains. As the hybridization results of their genomic DNAs with pcbABCD as the DNA probe, the group of Pseudomonas sp. DJ-12. P08 and P27 strain was separated by the group of P20 and P1242 strains. The P. pseudoalcaligenes KF707 showed the hybidization signal which was homologous to the group of DJ-12, but they had different restriction endonuclease sites. The pcbAB genes in pCUl recombinant plasmid from Pseudomonas sp. DJ-12 appeared to be homologous to pchAB genes in pKTF20 cloned from P. pseudoalcaligenes KF707, but the C genes in both strains were not homologous. The bphABC in pKTF20 showed the signals homologous to the cbp ACB in pAW6194 cloned from P. putida OU83, but homologous signal was not found botween the pcbABCD genes in pCUl and the cbpADCB genes in pAW6194 recombbinant plasmid.

Keywords

References

  1. J. Gen. Microbiol. v.137 Bioconversion of 2-hydroxy-6-oxo-6-(4'chlorophenyl)hexa-2,4-dienoic acid, the metacleavage product of 4-chlorobiphenyl Ahmad,D.;M.Sylvestre;M.Sondossi;R.Masse
  2. J. Bacteriol. v.175 Three different 2,3-dihydroxybiphenyl-1,2-dioxygenase genes in the Gram-positive polychlorobiphenyl-degrading bacterium Rhodococcus globerulus P6 Asturias,J.;K.N.Timmis
  3. Current protocols in molecular biology Ausubel,F.M.;R.Brent;R.E.Kingston;D.D.Moore;J.G.Seidman;J.A.Smith;K.Struhl
  4. J. Bacteriol. v.175 Gene components responsible for discrete substrate specificity in the metabolism of biphenyl(bph operon) and toluene(tod operon) Furukawa,K.;J.Hirose;A.Suyama;T.Zaika;S.Hayashida
  5. J. Bacteriol. v.171 Molecular relationship of chromosomal genes encoding biphenyl/ polychlorinated biphenyl catabolism: Some soil bacteria possess a highly conserved bph operon Furukawa,K.;N.Hayase;K.Taira;N.Tomizuka
  6. J. Bacteriol. v.166 Cloning of gene cluster encoding biphenyl and chlorobiphenyl degradation in Pseudomonas pseudoalcaligens Furukawa,K.;T.Miyazaki
  7. Kor. J. Microbiol. v.31 Cloning and expression of pcbAB genes from Pseudomonas sp. DJ-12 in Escherichia coli Han,J.J.;T.K.Sung;C.K.Kim
  8. J. Bacteriol. v.172 Pseudomonas putida KF715 bphABCD operon encoding biphenyl and polychlorinated biphenyl degradation: cloning, analysis and expression in soil bacteria Hayase,N.;K.Taira;K.Furukawa
  9. Appl. Environ. Microbiol. v.55 Cloning of bacterial genes specifying degradation of 4-chlorobiphenyl from Pseudomonas putida OU83 Khan,A.;S.Walia
  10. Appl. Environ. Microbiol. v.57 Expression, localization and functional analysis of polychlorinated biphenyl degradation genes cbpABCD of Pseudomonas putida Khan,A.;S.Walia
  11. Kor. J. Microbiol. v.25 Isolation and characterization of bacteria degrading chlorinated aromatic hydrocarbons Kim,C.K.;J.W.Kim;Y.C.Kim
  12. Kor. J. Microbiol. v.32 Cloning and expression of Pseudomonas sp. DJ-12 pcbCD genes in Escherichia coli Kim,C.K.;T.K.Sung;J.H.Nam;Y.C.Kim;J.K.Lee
  13. Molecular cloning(2nd ed.) Sambrook,J.;E.F.Fritsch;T.Maniatis
  14. J. Mol. Biol. v.98 Detection of specific segments among DNA fragments separated by gel electrophoresis Southern,E.M.
  15. Kor. J. Microbiol. Biotechnol. v.21 Enzymatic properties of the 2,3-dihydroxybiphenyl dioxygenase purified from Pseudomonas sp. DJ-12 Sung,T.K.;J.H.Nam;C.K.Kim
  16. Biochem. v.27 Cloning and nucleotide sequence of the 2,3-dihydroxybiphenyl dioxygenase gene from the PCB-degrading strain of Pseudomonas paucimobilis Q1 Taira,K.;N.Hayase;N.Arimura;S.Yamashita;T.Miyazaki;K.Furukawa
  17. Appl. Environ. Microbiol. v.56 Construction and applications of DNA probes for detection of polychlorinated biphenyl-degrading genotypes in toxic organic-contaminated soil environments Walia,S.;A.Khan;N.Rosenthal
  18. J. Bacteriol. v.171 Sequence similarities in the genes encoding polychlorinated biphenyl degradation by Pseudomonas strain LB400 and Alcaligenes eutrophus H850 Yates,J.R.;F.J.Mondello
  19. Kor. J. Microbiol. v.30 Divergence of the cbp genes in 4-chlorobiphenyl catabolizing bacteria Yun,D.J.;J.J.Han;C.K.Kim