• Title/Summary/Keyword: biochemical industry

Search Result 209, Processing Time 0.032 seconds

Effects of a lipid-encapsulated zinc oxide supplement on growth performance and intestinal morphology and digestive enzyme activities in weanling pigs

  • Jang, Insurk;Kwon, Chang Hoon;Ha, Duck Min;Jung, Dae Yun;Kang, Sun Young;Park, Man Jong;Han, Jeong Hee;Park, Byung-Chul;Lee, Chul Young
    • Journal of Animal Science and Technology
    • /
    • v.56 no.8
    • /
    • pp.29.1-29.6
    • /
    • 2014
  • This study compared the effects of varying lipid content and dietary concentration of a lipid-encapsulated (LE) ZnO product to those of native ZnO and thereby to find insights into optimal lipid coating and dosage of the Zn supplement. A total of 192 21-d-old weanling pigs were allotted to 48 pens, after which each six pens received a ZnO-free basal diet supplemented with 125 ppm ZnO (100 ppm Zn; BASAL), 2,500 ppm Zn as native ZnO (HIGH), or 100 or 200 ppm Zn as LE ZnO (LE-100 or LE-250) containing 8%, 10%, or 12% lipid [LE-8%, LE-10%, or LE-12%, respectively; $2{\times}3$ factorial arrangement within the LE-ZnO diets (LE-ALL)] for 14 d. Forty pigs were killed at the end for histological and biochemical examinations. None of ADG, ADFI, gain:feed, and fecal consistency score differed between the LE-ALL and either of the BASAL and HIGH groups. Hepatic and serum Zn concentrations were greater (p <0.05) in the HIGH vs. LE-ALL group, but did not differ between LE-ALL and BASAL, between LE-100 and -250, or among LE-8%, -10%, and -12% groups. Villus height (VH), crypt depth (CD), and the VH:CD ratio in the duodenum, jejunum, and ileum did not differ between the LE-ALL and either of the BASAL and HIGH groups, except for a greater CD in the duodenum in the LE-ALL vs. HIGH group. Additionally, VH and CD in the duodenum and VH:CD in the jejunum were greater in the LE-250 vs. LE-100 group. Specific activities of sucrase, maltase, and leucine aminopeptidase in these intestinal regions and those of amylase and trypsin in the pancreas were not influenced by the lipid content or dietary concentration of LE ZnO and also did not differ between the LE-ALL and either of the BASAL and HIGH groups, except for a greater pancreatic amylase activity in the former vs. HIGH group. In conclusion, the present results indicate that the LE ZnO, regardless of its lipid percentage or supplementation level examined in this study, has no significant effect on growth performance, fecal consistency, or digestive enzyme activities of weanling pigs under the experimental conditions.

Present Status and Prospects of Marine Chemical Bioindustries (해양화학생물산업의 현황과 전망)

  • Lee, Sun-Bok;Cho, Sun-Ja;Lee, Shin-Youb;Paek, Kwang-Hyun;Kim, Jeong-A;Chang, Jin-Hwa
    • KSBB Journal
    • /
    • v.24 no.6
    • /
    • pp.495-507
    • /
    • 2009
  • As we move into the 21st century, the importance of marine resources is certain to increase due to the accelerated exhaustion of land resources. For the sustainable development of the world, therefore, we need to develop marine chemical bioindustries which enable us to produce industrial chemicals, advanced materials, fuels, and minerals from marine resources such as seaweeds and seawater. In this review, we selected five marine chemical bioindustries which include 1) seaweed polysaccharide industry, 2) marine advanced materials industry, 3) marine biofuel industry, 4) marine sea salt industry, and 5) deep-sea water industry, and discussed the current status and future prospects of each industry sector. It has been assessed that the future of marine chemical bioindustry looks very promising although there are many needs for more intensive research investments on marine bioprocess development through close cooperation between marine biologists and biochemical engineers.

Fabrication and Characterization of PVA/CMC Hydrogels by Freezing-Thawing Technique and Gamma-Ray Irradiation (동결/융해와 방사선 가교법에 의한 PVA/CMC 수화젤의 제조 및 특성 평가)

  • Jo, Sun-Young;Lim, Youn-Mook;Youn, Min-Ho;Gwon, Hui-Jeong;Park, Jong-Seok;Nho, Young-Chang;Shin, Heung-Soo
    • Polymer(Korea)
    • /
    • v.33 no.6
    • /
    • pp.551-554
    • /
    • 2009
  • Poly (vinyl alcohol) (PVA) and carboxymethyl cellulose (CMC) have received increasing attention in biomedical and biochemical applications because of their properties such as being water-soluble and biocompatible. In this study, a PVA/CMC hydrogel applicable to artificial cartilage was prepared by a freezing-thawing technique and a gamma-ray irradiation. The solid concentration of PVA was 7 wt% and the concentration of CMC was 4 wt%. The freezing/thawing process was repeated twice and the dose of gamma-ray irradiated was 30 kGy. Results of gelation before and after gamma-ray irradiation were similar, but the swelling degree decreased and compressive strength increased. The cytotoxicity was investigated with CCK-8 assay.

Comparison of Microbial Community Compositions between Doenjang and Cheonggukjang Using Next Generation Sequencing (차세대 염기서열 분석법을 이용한 전통 된장과 청국장의 미생물 분포 분석)

  • Ha, Gwangsu;Kim, JinWon;Shin, Su-Jin;Jeong, Su-Ji;Yang, Hee-Jong;Jeong, Do-Youn
    • Journal of Life Science
    • /
    • v.31 no.10
    • /
    • pp.922-928
    • /
    • 2021
  • To profile the microbial compositions of Korean traditional fermented paste made from whole soybeans, Doenjang and Cheonggukjang, and compare their taxonomic differences, we analyzed the V3-V4 region of 16S rRNA of naturally fermented foods by using next generation sequencing. α-Diversity results showed that values indicating bacterial community abundances (OTUs) and richness (ACE, Chao1) were statistically significant (p=0.0001) in Doenjang and Cheonggukjang. Firmicutes was the most common phylum in both groups, representing 97.02% and 99.67% in the Doenjang and Cheonggukjang groups, respectively. Bacillus was the most dominant genus, accounting for 71.70% and 59.87% in both groups. Linear discriminant (LDA) effect size (LEfSe) analysis was performed to reveal the significant ranking of abundant taxa in different fermented foods. A size-effect threshold of 2.0 on the logarithmic LDA score was used for discriminative functional biomarkers. On the species level, Bacillus subtilis, Tetragenococcus halophilus, and Clostridium arbusti were significantly more abundant in Doenjang than in Cheonggukjang, whereas Bacillus thermoamylovorans, Enterococcus faecium, and Lactobacillus sakei were significantly more abundant in Cheonggukjang than in Doenjang. Permutational multivariate analysis of variance (PERMANOVA) showed that the statistical difference in microbial clusters between the two groups was significant at the confidence level of p=0.001. This research could be used as basic research to identify the correlation between the biochemical characteristics of Korean fermented foods and the distribution of microbial communities.

Screening of Non-Biogenic-Amine-Producing Bacillus subtilis and Medium Optimization for Improving Biomass by the Response Surface Methodology (바이오제닉 아민 비생성 Bacillus subtilis의 선별 및 반응표면 분석법에 의한 균체량 증가를 위한 배지 최적화)

  • Yang, Hee-Jong;Jeong, Su-Ji;Jeong, Seong-Yeop;Heo, Ju-Hee;Choi, Nack-Shick;Jeong, Do-Youn
    • Journal of Life Science
    • /
    • v.26 no.5
    • /
    • pp.571-583
    • /
    • 2016
  • Biogenic amines are produced primarily by microorganisms found in fermented foods and are often implicated in poisoning incidents in humans. In this study, 620 strains of microorganisms were isolated from traditional Korean fermented food in Sunchang in order to screen for non-biogenicamine-producing microorganisms present in these foods. One strain was identified and named Bacillus subtilis SCJ1, by using 16S rRNA sequencing and biochemical characterization. We investigated the cell growth of this organism in order to understand its potential for industrial application. To this end, we optimized the culture medium constituents by using the response surface methodology. The Plackett-Burman experimental design was used for screening of the medium constituents, such as molasses, yeast extract and peptone, for improving cell growth. In order to determine the optimal concentration of each constituent, we used a central composite design. Consequently, the optimized concentrations of molasses, yeast extract and peptone were predicted to be 27.5 g/l, 7.5 g/l and 17.5 g/l, respectively. By model verification, we confirmed that a 1.49-fold increase in dry cell weight compared to the basal medium-from 1.32 g/l, to 1.9722 g/l-was achieved.

Spoilage Lactic Acid Bacteria in the Brewing Industry

  • Xu, Zhenbo;Luo, Yuting;Mao, Yuzhu;Peng, Ruixin;Chen, Jinxuan;Soteyome, Thanapop;Bai, Caiying;Chen, Ling;Liang, Yi;Su, Jianyu;Wang, Kan;Liu, Junyan;Kjellerup, Birthe V.
    • Journal of Microbiology and Biotechnology
    • /
    • v.30 no.7
    • /
    • pp.955-961
    • /
    • 2020
  • Lactic acid bacteria (LAB) have caused many microbiological incidents in the brewing industry, resulting in severe economic loss. Meanwhile, traditional culturing method for detecting LAB are time-consuming for brewers. The present review introduces LAB as spoilage microbes in daily life, with focus on LAB in the brewing industry, targeting at the spoilage mechanism of LAB in brewing industry including the special metabolisms, the exist of the viable but nonculturable (VBNC) state and the hop resistance. At the same time, this review compares the traditional and novel rapid detection methods for these microorganisms which may provide innovative control and detection strategies for preventing alcoholic beverage spoilage, such as improvement of microbiological quality control using advanced culture media or different isothermal amplification methods.

Ganoderma lucidum균 전처리를 이용한 볏짚의 상압.소다펄프화

  • 주용찬;강진하
    • Journal of Korea Technical Association of The Pulp and Paper Industry
    • /
    • v.34 no.4
    • /
    • pp.51-60
    • /
    • 2002
  • This study was carried out to develop the biochemical pulping method to enhance energy saving and decrease the capital cost through the soda pulping under atmospheric pressure (100%). Nonwood substrates, rice straw, were pretreated by white-rot fungi, Ganoderma Iucidum. It has acquired several basic data that can be applied in bio-soda pulping. The results of this study were as follow. Without any nutrients or with glucose, N and glucose+N the weight losses of rice straws inoculated by Canoderma Iucidum were 8.5~29.8%, 9.3~32% 11.8~30.1% and 11.8~24.4% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The more extending incubation periods, the more increasing weight losses. The yield of untreated rice saw was 54.8% after pulping. When any nutrients was not added or glucose, N and glucose+N were added for the pretreatment, the total yields were ranged 50.6~38.5%, 48.6~34.4%, 47.2~38.4% and 49.5~42.6% respectively for 5, 10, 15, 20, 25 and 30 days incubation. The yields were gradually decreased based on extending of incubation. The physical properties of rice straw soda pulps without fungal treatment, the density, breaking length, burst index, tear index and folding endurance were 0.24 g/$\textrm{cm}^3$, 2.32 km, 0.91 kPa $m^2$/g, 56.7 mN.$m^2$/g and 35 times, respectively. After pretreatment without any nutrients or with glucose, N and glucose+N as nutrients the density was 0.27~0.30 g/$\textrm{cm}^3$, the breaking length 3.14~5.25 km, burst index 1.42~2.78 kPa.$\textrm{m}^2$/g, tear index45.8~64.5 mN.$\textrm{m}^2$/g and folding endurance 47~288 times at all incubating periods when pulping was done. The physical properties were increased with the increasing incubation periods. However, when glucose+N was added, the physical properties were shown superior results each incubating duration.

The Relationship between Some Blood Parameters and Antioxidant Enzyme Activity in Korean Postmenopausal Women (일부 폐경 후 여성에서 영양상태 및 골대사 관련 일부 혈액 지표와 항산화효소 활성과의 관련성 분석)

  • Lee, Haeng-Shin;Kim, Mi-Hyun;Lee, Da-Hong;Sung, Chung-Ja
    • Journal of Nutrition and Health
    • /
    • v.39 no.5
    • /
    • pp.476-484
    • /
    • 2006
  • To elucidate the relationship between blood parameters related bone metabolism and antioxidant enzyme activity in postmenopausal period 60 women residing in Iksan area were recruited. Food and nutrient intake of each individual subject were estimated by 24-hour recalls of 3 non-consecutive days. The biochemical markers including total protein, albumin, osteocalcin (intact bone gla protein; BOP), calcium, phosphorus and hemoglobin were measured in fasting blood. In addition, parameters of antioxidative capacity including the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) , catalase (CAT) and total antioxidant capacity (TA) were monitored in blood, also. The mean age, height, weight, and BM! of subjects were 64.8 years, 151.1 em, 59.5 kg $26.0\;kg/m^2$, respectively. The mean SOD, GPx, and CAT activities were 138.5 U/ml, 1,273.8 U/ml and 314.3 kU/l respectively, and TA was 1.16 mmol/l without significant difference among different age groups. BMI was positively correlated with SOD activity (p < 0.01). SOD activity and CAT activity showed positive correlation with serum albumin (p < 0.05) and hemoglobin (p < 0.01). In conclusion, this study revealed that antioxidant enzyme activity holds a significant relationship with the blood parameters like as serum albumin and hemoglobin in postmenopausal women and further systematic research is needed to investigate the their relation mechanism.

Characterization of Lactobacillus brevis JBE 30 as a Starter for the Brewing of Traditional Liquor (전통주 양조에 적합한 Lactobacillus brevis JBE 30 균주의 특성)

  • Heo, Jun;Ryu, MyeongSeon;Jeon, SaeBom;Oh, HyeonHwa;Kim, Young Sang;Jeong, DoYoun;Uhm, Tai-Boong
    • Korean Journal of Microbiology
    • /
    • v.50 no.3
    • /
    • pp.233-238
    • /
    • 2014
  • For the collection of starters suitable for the brewing of traditional liquor, an alcohol-resistant strain of lactic acid bacteria with low level of acid production was isolated from traditional fermented soybean lumps. The strain named as JBE 30 was identified as Lactobacillus brevis by 16S rRNA sequence analysis and additional biochemical tests. The strain could grow well at a MRS medium containing 8% (v/v) ethanol for 96 h of cultivation at $30^{\circ}C$. The final pH after cultivation was 4.5. It also inhibited the growth of food spoilage and pathogenic bacteria including Escherichia coli, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus, Micrococcus luteus, and Pseudomonas aeruginosa. These results showed that Lactobacillus brevis JBE 30 could be used as a promising starter in brewing process of traditional liquor.

Assessment of the Applicability of Convergence Technology for Reducing and Blocking Pollution Loads to Rivers through the Utilization of Waterfront Spaces (수변공간을 활용한 오염부하 저감 및 차단 융복합 기술의 하천 적용성 평가)

  • Kim, Bong Kyun;Seo, Dae Seuk;Oh, Jong Min;Park, Jae-Ro
    • Ecology and Resilient Infrastructure
    • /
    • v.3 no.4
    • /
    • pp.238-246
    • /
    • 2016
  • Water purification facilities utilizing three technologies - a detention pond, an artificial wetland and an ecological revetment - were installed in waterfront spaces, including river embankments and watersides that have so far been left neglected, and then their water purification levels were analyzed in this study. The water purification facilities were found to show average removal efficiencies of 48.6% in suspended solid (SS), 30.5% in biochemical oxygen demand (BOD), 18.4% in total nitrogen (T-N) and 27.3% in total phosphorus (T-P) during rainfall. The removal efficiencies during non-rainfall were 33.2% in SS, 28.6% in BOD, 13.7% in T-N and 17.3% in T-P. These results showed that the water purification facilities using a detention pond, an artificial wetland and an ecological revetment can be used as a useful natural water purification technology in in waterfront spaces.