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Conformational Switch of the Strained Native Serpin Induced by Chemical Cleavage of the Reactive Center Loop

  • Im, Ha-Na;Yu, Myeong-Hee
    • BMB Reports
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    • v.33 no.5
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    • pp.379-384
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    • 2000
  • The native conformation of serpins (serine protease inhibitors) is strained. Upon cleavage of the reactive center loop of serpins by a protease, the amino terminal portion of the cleaved loop is inserted into the central ${\beta}-sheet$, A sheet, as the fourth strand, with the concomitant release of the native strain. We questioned the role of protease in this conformational switch from the strained native form into a stable relaxed state. Chemical cleavage of the reactive center loop of ${\alpha}_1-antitrypsin$, a prototype serpin, using hydroxylamine dramatically increased the stability of the serpin. A circular dichroism spectrum and peptide binding study suggests that the amino terminal portion of the reactive center loop is inserted into the A sheet in the chemically-cleaved ${\alpha}_1-antitrypsin$, as in the enzymatically-cleaved molecule. These results indicate that the structural transformation of a serpin molecule does not require interaction with a protease. The results suggest that the serpin conformational switch that occurred during the complex formation with a target protease is induced by the cleavage of the reactive center loop per se.

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Development of a Composite Fiber Reinforcement Pavement using Eco-Friendly Grid and Dispersive Fibers (친환경 쉬트형 보강재 및 분산성 섬유를 적용한 복합 섬유 보강 포장 개발)

  • Park, Ju Won;Kim, Hyeong Su;Kim, Hyeok Jung;Kim, Sung Bo
    • International Journal of Highway Engineering
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    • v.19 no.6
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    • pp.57-66
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    • 2017
  • PURPOSES : This study develops eco-friendly asphalt reinforcement materials applicable to bridge deck pavement. The main purpose is to ensure highly reliable quality applicable to structures and the possibility of practical application. The main target of the study is to develop materials that are environmentally friendly and capable of improving performance. METHODS : The application of double-reinforcement fiber improves the performance of the road pavement. 1. We use recycled film for application of sheet-typed reinforcement. 2. We use preprocessing fibers to reinforce the properties of composite pavement materials. RESULTS : The developed products may produce materials that fit the purpose of achieving stability and environmental friendliness. Sheet-typed reinforcements use more than 50% recycled resin. The most important type of damage to the asphalt layer is deflection (plastic deformation). These products have a very high deflection resistance of not less than 6,000 cycles/mm. In addition, all performance is excellent. Thus, it will be easier to access the field in the future. CONCLUSIONS : Fiber-reinforced asphalt pavement showed excellent performance. Sheet-typed reinforcements containing 50% recycling resin produced good performance in terms of functionality as well as environmental friendliness. Thus, enhancing the field applicability will enhance the usability of the reinforcements.

Development of Nano Convergence Films Using a Roll-to-Roll Coating System

  • Hwang, Joong Kook;Chang, Sang-Mok;Shin, Hoon-Kyu
    • Transactions on Electrical and Electronic Materials
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    • v.17 no.3
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    • pp.168-171
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    • 2016
  • There has been growing interest and rapid development in transparent electrode films, which are flexible and light and used in mobile, simple information, and electronic devices, and based on recent advancements in nano technology, information technology, and display technology. In particular, studies on developing such films with both high conductivity and high transmittance of visible rays are highly in demand for commercialization. In this study, transparent electrode films were developed for IT using micro patterns that show sheet resistance less than 10 Ω/□, adhesive strength more than 98%, and light transmittance more than 90%. The results of applying a surface emission gradient minimization (Honey Comb) technology to the films was the verification of the sheet resistance, adhesive strength, and light transmittance satisfying the target level of this study through Imprinting and Remolding processes.

Properties of ZnO:Ga thin films deposited by RF magnetron sputtering under various RF power

  • Kim, Deok Kyu;Kim, Hong Bae
    • Applied Science and Convergence Technology
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    • v.24 no.6
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    • pp.242-244
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    • 2015
  • ZnO:Ga thin films were deposited by RF magnetron sputtering technique from ZnO (3 wt.% $Ga_2O_3$) target onto glass substrates under various RF power. The influence of RF power on the structural, electrical, and optical properties of ZnO:Ga thin films was investigated by X-ray diffraction, atomic force microscopy, Hall method and optical transmission spectroscopy. As the RF power increases from 50 to 110W, the crystallinity is deteriorated, the root main square surface roughness is decreased and the sheet resistance is increased. The increase of sheet resistance is caused by decreasing carrier concentration due to interstitial Ga ion. All films are transparent up to 80% in the visible wavelength range and the adsorption edge is a red-shift with increasing RF power.

Study on the Cold Stamping Process Design Method of 1.5GPa Grade Front Side Rear Lower Member (1.5GPa급 Front Side Rear Lower Member 냉간 성형공정 설계기법 연구)

  • Nam, S.W.;Bae, G.H.
    • Transactions of Materials Processing
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    • v.30 no.5
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    • pp.236-241
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    • 2021
  • This study describes the cold stamping process design procedure to secure the formability and dimensional accuracy of the automotive structural component fabricated by 1.5GPa grade ultra-high strength steel sheet. The target product is selected as the front side rear lower member which is the most important energy absorption part in the frontal impact condition. To secure the product quality, an intermediate product shape is added while considering the low elongation and high strength characteristics of 1470Mart. The sequential optimization procedure of the intermediate product shape, the fine dimensional quality is then achieved without any crack or wrinkling. The cold stamping method with ultra-high strength steel sheets is validated by conducting the die tryout of the front side rear lower member.

Improvement of PDMS graphene transfer method through surface modification of target substrate (폴리디메틸실록산(PDMS)을 이용한 그래핀 전사법 개선을 위한 계면처리 연구)

  • Han, Jae-Hyung;Choi, Mu-Han
    • Journal of the Korean Applied Science and Technology
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    • v.32 no.2
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    • pp.232-239
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    • 2015
  • In this paper, we study the dry transfer technology utilizing PDMS (Polydimethylsiloxane) stamp of a large single-layer graphene grown on Cu-foil as catalytic metal by using Chemical Vapor Deposition (CVD). By changing the surface property of the target substrate through $UV/O_3$ treatment, we can transfer the graphene on the target substrate while minimizing mechanical damages of graphene layer. Multi-layer (1~4 layers) graphene was stacked on $SiO_2/Si$ wafer successfully by repeating thetransfer method/process and then optical transmittance and sheet resistance of graphene layers have been measured as a quality assessment.

Characteristics for VOCs and aldehydes emission rates from architectural flooring (건축용 바닥재로부터의 VOCs와 Aldehydes 방출 특성)

  • Jang, Seong-Ki;Kim, Mi-Hyun;Seo, Soo-Yun;Lee, Woo-Suk;Lim, Jun-Ho;Lim, Jeong-Yun
    • Analytical Science and Technology
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    • v.19 no.6
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    • pp.544-552
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    • 2006
  • Emission tests were carried out to investigate the characteristics of concentration according to flooring sort using small chamber method. The target Volatile Organic Compounds (VOC) included 27 individual compounds of environmental concern, which were determined by adsorption sampling and thermal desorption coupled with GC/MS method and by DNPH cartridge/HPLC method. The emission factor of Total Volatile Organic Compounds (TVOC) and Formaldehyde (HCHO) was detected $0.3mg/m^2{\cdot}h$ and $0.2mg/m^2{\cdot}h$ respectively, and the floorings of 37 (9 PVC Tile, 10 PVC Sheet, 18 Flooring) were satisfied emission standard. TVOC emission factor appeared in order of concentration of PVC Sheet, PVC Tile, and floor flooring, while HCHO was detected very high emission factor (as $0.4mg/m^2{\cdot}h$) at floor flooring above PVC series (as $0.001mg/m^2{\cdot}h$).

Recent progress in oxide phosphor thin-film electroluminescent devices

  • Minami, Tadatsugu;Miyata, Toshihiro
    • 한국정보디스플레이학회:학술대회논문집
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    • 2006.08a
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    • pp.27-32
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    • 2006
  • The present status and prospects for further development of thin-film electroluminescent (TFEL) devices using oxide phosphors are described. High-luminance oxide TFEL devices have been recently developed using a new combinatorial deposition technique featuring rf magnetron sputtering with a subdivided powder target. In addition, new flexible oxide TFEL devices have been fabricated on an oxide ceramic sheet and operated stably in air above $200^{\circ}C$.

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Superplastic Forming /Diffusion Bonding Processes Design Using a Finite Element Method (유한요소법을 이용한 초소성 성형/확산접합 공정 설계)

  • 홍성석;이종수;김용환
    • Proceedings of the Korean Society for Technology of Plasticity Conference
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    • 1995.03a
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    • pp.155-161
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    • 1995
  • Superplastic forming/diffusion bonding(SPF/DB) processes are analyzed using a rigid visco-plastic finite element method. The optimum pressure-time relationship for a target strain rate and thickness distributions were predicted using two-node line element based on membrane approximation for plane strain shapes. Material behavior during SPF/DB of the integral structures with complicated shapes are investigated. The tying condition is employed for the analysis inter-sheet contact problems. A movement of rib structure is successfully prodicted during the forming.

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Finite Element Analysis of Superplastic Forming/Diffusion Bonding Processes (초소성 성형/확산접합 공정의 유한요소 해석)

  • 홍성석;김용환
    • Transactions of Materials Processing
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    • v.5 no.1
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    • pp.37-46
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    • 1996
  • Superplastic forming/diffusion bonding (SPF/DB) processes were analyzed using a rigid visco-plastic finite element method. The optimum pressure-time relationship for a target strain rate and thickness distributions were predicted by two-node line elements based on the membrane approximation for plane strain. Material behavior during SPF/DB of the integral structures having complicated shapes was investigated. The tying condition is employed for the analysis of inter-sheet contact problems. A movement of rib structure is successfully predicted during the forming.

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