• 식물병연구
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• 제10권1호
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• pp.30-33
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• 2004

현재까지 벼 줄무의잎마름병(Rice stripe virus, RSV)은 남부지방에 국한되어 발생되어 왔다. 그러나 최근에는 RSV의 발생이 충청도, 경기도를 포함한 중부지방까지 확산되는 경향을 나타내고 있다. 이병의 병징은 육안으로는 생리적인 장해 현상과 구분하기가 힘들다 본 실험에서는 이병주 및 애멸구(Laodelphax striatellus)로 부터 viral RNA를 추출한 후 RNA복제효소 및 외피단백질유전자에 특이적인 primer를 제작하여 RT-PCR법에 의해 RSV를 검정하였다. 그결과 이병식물체 및 보독 애멸구로부터 RNA복제효소 유전자에 특이적인 band(1,023 bp) 및 외피단백질유전자에 특이적인 band(969 bp)가 관찰되었다.

• 한국응용곤충학회지
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• 제54권4호
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• pp.419-423
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• 2015

애멸구(Laodelphax striatellus)는 rice stripe virus (RSV)의 매개충으로 벼에 큰 피해를 주는 해충이다. 본 연구에서는 애멸구의 장 단시형, 암 수, 약 성충에 대한 RSV 보독률과 이병률을 비교하였다. 애멸구의 장 단시형의 RSV 보독률은 각각 60.7%, 63.1%로 크게 차이는 없었다. 암 수에 대한 RSV 보독률은 각각 61.9%, 52.2%로 암컷의 보독률이 더 높았으나 유의성은 없었다. 약 성충의 보독률을 비교한 결과 각각 51.2%, 58.7%로 역시 크게 차이가 나지는 않았다. RSV에 감염된 애멸구에 노출된 건전한 벼의 이병률은 장시형은 53.3%, 단시형은 48.2%를 보였으며, 약 성충의 이병률은 각각 38.2%, 42.6%를 보여 유의성은 없었다. 반면 암컷은 50.5%의 이병률을 보이고 수컷은 22.3%의 이병률을 보여 암컷이 수컷에 비해 22.3% 이병률이 높아 유의성이 있었다. 또한 벼와 애멸구의 RSV 감염여부에 의한 애멸구의 발육기간은 건전한 벼에 RSV 감염 애멸구를 접종 했을 때 가장 긴 것으로 나타났으며, 건전한 벼에 건전한 애멸구를 접종 했을 때 발육기간이 가장 짧은 것으로 나타났다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.140.2-141
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• 2003

Among over-wintering small brown planthoppers, population of the rice stripe virus (RSV)-viruliferous insects was surveyed throughout the country in late April of 2003 by using DAS-ELISA. Averaged population of the RSV-viruliferous insects in this year was 2.1％, which was lower than that of last year of 3.7％. However, the insect population in Seoul, Incheon and Kyeonggi areas were relatively high showing 6.7％, 6.2％ and 2.6％, respectively. Based on the survey results, it was expected that overall occurrence of RSV on rice could be decreased in this year, except certain areas. Ovarial transmission rate of RSV by the insects on diseased rice samples collected from 10 areas ranged from 22.2％ to 77.8％. Among 35 graminous weed species collected from rice fields in Ganghwa and Kimpo in 2002 and 2003, common reed and formosens were found to be infected by RSV. The result indicates that those weeds are potential alternative natural hosts of the RSV Further studies on ecological and pathological impacts of the alternative natural host of RSV are being processed.

• The Plant Pathology Journal
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• 제29권3호
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• pp.223-233
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• 2013

Rice stripe virus (RSV) is one of the most destructive viruses of rice, and greatly reduces rice production in China, Japan, and Korea, where mostly japonica cultivars of rice are grown. RSV is transmitted by the small brown plant-hopper (SBPH) in a persistent and circulative-propagative manner. Several methods have been developed for detection of RSV, which is composed of four single-stranded RNAs that encode seven proteins. Genome sequence data and comparative phylogenetic analysis have been used to identify the origin and diversity of RSV isolates. Several rice varieties resistant to RSV have been selected and QTL analysis and fine mapping have been intensively performed to map RSV resistance loci or genes. RSV genes have been used to generate several genetically modified transgenic rice plants with RSV resistance. Recently, genome-wide transcriptome analyses and deep sequencing have been used to identify mRNAs and small RNAs involved in RSV infection; several rice host factors that interact with RSV proteins have also been identified. In this article, we review the current statues of RSV research and propose integrated approaches for the study of interactions among RSV, rice, and the SBPH.

• The Plant Pathology Journal
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• 제29권3호
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• pp.330-337
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• 2013

Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.

• The Plant Pathology Journal
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• 제20권4호
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• pp.313-315
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• 2004

The coat protein gene of Korean isolate of Ricer stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a protein of 322 amino acids. RSV-Kr showed 94%-99% sequence identities to that of Japanese- and Chinese isolates.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 추계국제학술대회
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• pp.63-63
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• 2020

Rice stripe virus (RSV) disease is one of the major constraints in rice production, transmitted by the small brown planthopper (SBPH; Laodelphax striatellus). Upon RSV infection, plants develop typical symptoms, which include chlorosis and weakness of newly emerged leaves, white and yellow spots, stripe on leaves, and necrotic and wilting leaves, resulting in plant growth inhibition, oxidative damage that may culminate in programmed cell death (PCD) and plant death in severe epidemics. Although RSV-resistant quantitative trait loci (QTLs), Stv-a, Stv-b, and Stv-bi, were mapped using various resistant varieties, one RSV-resistant gene, OsSOT1, has been identified so far. In this study, we used the rice cultivar Zenith, known to carry Stv-b, to investigate novel RSV-genes through fine mapping. Therefore, we crossed Zenith (Donor parent, RSV resistant) with Ilpum (Recurrent parent, RSV susceptible) to fine-map using a BC₂F₂ population of 2100 plants. Chromosome segment introgression lines that were heterozygous at a different region were selected, two types of heterozygous lines showed an heterozygous genotype between Sid2 and Sid75 to Indel9 and RM6680. Interestingly, we identified qSTV11^Z region harboring Stv-b, covering about 171-kb region between the InDel markers Sid75 and Indel8. The localization of qSTV11^Z provides useful information that could be used for marker-assisted selection and determination of genetic resources in rice breeding.

• 식물병연구
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• 제18권3호
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• pp.245-249
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• 2012

애멸구는 벼 생산에 있어서 흡즙에 의한 피해를 일으킬 뿐 아니라 벼줄무늬잎마름바이러스를 매개하는 매우 중요한 해충이다. 벼줄무늬잎마름병의 발생은 애멸구 발생과 매우 일치하므로 벼 농사에 벼줄무늬잎마름병 예방에 애멸구 관리가 매우 중요하다. 본 연구는 2011년 6월 충남 태안읍 근흥면 고남면 일대에 설치된 공중포충만을 이용하여 애멸구의 이동 및 바이러스 보독률을 조사하였으며, 애멸구 대량비래 후, 살충제(동부한농 etofenprox(10%))의 처리 횟수, 처리 시기에 따른 벼줄무늬잎마름병의 보독률을 조사하였다. 태안군 일대에서 애멸구 대량 비래후 약 1,217마리가 포획되었으며 이중 7.7%는 벼줄무늬잎마름바이러스, 4.4%는 벼검은줄오갈바이러스의 보독률을 보였다. 대량비래 후 2차례 살충제 처리는 벼줄무늬잎마름병 보독률의 변화가 1차례 처리와 차이는 없었으나 처리 시기를 대량 비래후 5-7일 사이에 처리하였을 경우 RSV의 피해감소 효과를 무처리 대비 87.6%까지 감소시킬 수 있었다. 따라서 본 연구 결과는 애멸구 대량 비래후 5-7일 사이에 한 번의 살충제 처리가 가장 효과적인 벼줄무늬잎마름바이러스 방제라 하겠다.

• The Plant Pathology Journal
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• 제27권1호
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• pp.37-43
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• 2011

Rice stripe virus (RSV) is one of serious epidemic pathogens for rice species grown in many Asian countries. Therefore, it is necessary to produce a diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary, the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation, protein purification, and western blot analysis.

• The Plant Pathology Journal
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• 제27권2호
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• pp.148-155
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• 2011

Our previous sequence and phylogenetic analyses of the Korean Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSV-RNA segment variations, we developed a reverse transcription-polymerase reaction/restriction enzyme (RT-PCR/RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that the migratory small brown planthopper from Eastern China caught by aerial net traps that possesses RSV-RNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/RE, sequence and phylogenetic analyses, indicating that RSV populations in Eastern China may also have strong RNA variation. The development of an RE analysisbased method proved a useful epidemiological tool for rapid genotyping and identification of mixed infections by RSV strain and by different subtype.