The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus

  • Hong, Yeon-Kyu (Yeongnam Agricultural Research Institute, NICS, RDA.) ;
  • Kwak, Do-Yeon (Yeongnam Agricultural Research Institute, NICS, RDA.) ;
  • Park, Sung-Tae (Yeongnam Agricultural Research Institute, NICS, RDA.) ;
  • Choi, Jo-Im (Graduate School of Agricutural Biology, Kyungpook National University) ;
  • Lee, Key-Woon (Graduate School of Agricutural Biology, Kyungpook National University) ;
  • Lee, Bong-Choon (Yeongnam Agricultural Research Institute, NICS, RDA.)
  • Published : 2004.12.31
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Abstract

The coat protein gene of Korean isolate of Ricer stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a protein of 322 amino acids. RSV-Kr showed 94%-99% sequence identities to that of Japanese- and Chinese isolates.

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References

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