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http://dx.doi.org/10.5423/PPJ.2004.20.4.313

Cloning and Sequencing of Coat Protein Gene of the Korean Isolate of Rice stripe virus  

Hong, Yeon-Kyu (Yeongnam Agricultural Research Institute, NICS, RDA.)
Kwak, Do-Yeon (Yeongnam Agricultural Research Institute, NICS, RDA.)
Park, Sung-Tae (Yeongnam Agricultural Research Institute, NICS, RDA.)
Choi, Jo-Im (Graduate School of Agricutural Biology, Kyungpook National University)
Lee, Key-Woon (Graduate School of Agricutural Biology, Kyungpook National University)
Lee, Bong-Choon (Yeongnam Agricultural Research Institute, NICS, RDA.)
Publication Information
The Plant Pathology Journal / v.20, no.4, 2004 , pp. 313-315 More about this Journal
Abstract
The coat protein gene of Korean isolate of Ricer stripe virus (RSV-Kr) was cloned and its nucleotide sequence was determined. Total RNA was extracted from infected leaves and RSV viral RNA was detected by using RT-PCR with specific primer of coat protein gene. The result of RT-PCR showed a specific band. Purified RT-PCR products of coat protein gene were ligated into the pGEM-T Easy plasmid vector and cloned cDNA was obtained for nucleotide sequence determination. Coat protein gene of RSV-Kr consisted of 969 bp long encoding a protein of 322 amino acids. RSV-Kr showed 94%-99% sequence identities to that of Japanese- and Chinese isolates.
Keywords
Coat Protein; Rice stripe virus; sequence;
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