The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus

  • Lee, Joo Hyun (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Choi, Jae Young (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Tao, Xue Ying (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University) ;
  • Kim, Jae Su (Department of Agricultural Biology, College of Agricultural Life Sciences, Chonbuk National University) ;
  • Kim, Woojin (Research Institute for Agriculture and Life Sciences, Seoul National University) ;
  • Je, Yeon Ho (Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University)
  • Received : 2013.01.04
  • Accepted : 2013.03.07
  • Published : 2013.09.01
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Abstract

Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both libraries have similar GO structures, however, the gene expression pattern analysis revealed that 17.8% and 16.8% of isotigs were up- and down-regulated significantly in the RVLS, respectively. These RSV-dependently regulated genes possibly have important roles in the physiology of SBPH, transmission of RSV, and RSV and SBPH interaction.

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References

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