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http://dx.doi.org/10.5423/RPD.2004.10.1.030

Detection of Rice Stripe Virus using RT-PCR  

Lee, Bong-Choon (작물과학원, 영남농업연구소)
Hong, Yeon-Kyu (작물과학원, 영남농업연구소)
Kwak, Do-Yeon (작물과학원, 영남농업연구소)
Oh, Byeong-Geun (작물과학원, 영남농업연구소)
Park, Sung-Tae (작물과학원, 영남농업연구소)
Kim, Soon-Chul (작물과학원, 영남농업연구소)
Publication Information
Research in Plant Disease / v.10, no.1, 2004 , pp. 30-33 More about this Journal
Abstract
Until now, occurrence of rice stripe virus (RSV) is limitted in southern part of Korea. However, recently the occurrence of RSV is increased and spreaded in central part of Korea including Chungcheong and Kyonggi province. It is very difficult to distinguish RSV symptoms on virus symptom physiological damage of rice. We detected RSV viral RNA from infected rice and its insect vector Laodelphax striatellus using specific primer of RSV-polymerase and coat protein gene with reverse transcription (RT)-PCR. The result of RT-PCR, we observed specific band including RSV-polymerase (1,,023 bp) and CP (969 bp) in both host of rice and insect vector.
Keywords
Laodelphax striatellus; RSV; RT-PCR;
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