• Journal of Biosystems Engineering
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• 제40권3호
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• pp.277-283
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• 2015

Background: Foodborne disease outbreaks from various food sources are a major health concern worldwide. Current methods for detection of foodborne pathogens are both expensive and time-consuming. Purpose: This review aims to present the current information available on the use of lateral flow test strips to detect pathogens in food products to enhance food safety. Results: Frequent foodborne disease outbreaks from various food sources have increased the need for rapid and easy methods for routine analysis of foodborne pathogens. Present detection methods for foodborne pathogens require expensive instruments, experts, and long time for sample analysis. Lateral flow test strips have drawn attention in recent years because of their ability to detect analytes quickly and easily. This review focuses on the principle of the lateral flow test, the various formats of lateral flow test strips, recognition elements, labeling tags, and reading instruments. In addition, this review also discusses the future prospects for the lateral flow test strips.

• 한국잠사곤충학회지
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• 제50권2호
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• pp.189-193
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• 2012

To find diapause-related genes, we were performed by differential hybridization with three types of [${\alpha}-^{32}P$]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause-activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and $CO_2$ treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. $CO_2$ treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.

• 한국산업보건학회지
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• 제24권4호
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• pp.471-477
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• 2014

Objectives: This study was conducted to evaluate area exposure of airborne dust and organic gas during entrance and shipment of chicken in poultry farms Methods: Total dust was measured with NIOSH method 0500. The volume of distributed dust and organic gas(Ammonia, TVOC, Hydrogen Sulfide) were measured using direct reading instrument. Results: The range of concentrations of total dust in area sample was $0.07{\sim}4.91mg/m^3$ during the entrance of chicks and $4.37{\sim}9.4mg/m^3$ during shipment respectively. Concentration of ammonia reached approximately 9 ppm during shipment. There was a difference of concentration of total dust in the area sample between shipment and entrance tasks. Conclusions: It was found that the development of a special intervention program by type of task should be considered for reducing respiratory health effects among poultry farmers.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2192-2198
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• 2016

The myeloid-specific IgA Fc receptor ($Fc{\alpha}R$) is a cell surface molecule on immunocytes that provides a fundamental connection between humoral and cellular immunity. In this study, the full-length cDNA sequence of swine $Fc{\alpha}RI$ ($swFc{\alpha}RI$) was isolated and characterized and found to contain a 792-base-pair open reading frame, encoding a 264-amino-acid transmembrane glycoprotein with a predicted molecular mass of 29.4 kDa. The $swFc{\alpha}RI$ shares high amino acid sequence homology (>50%) with its counterparts from cattle, seal, and horse. Rosetting analysis confirmed that COS-7 cells transfected with an $swFc{\alpha}RI$ expression plasmid was able to combine with chicken erythrocytes sensitized with porcine IgA, but not IgG.

• 대한바이러스학회지
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• 제28권1호
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• pp.53-62
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• 1998

Human papillomavirus (HPV) 16, E7 proteins derived from the prototype (Bac73) and natural variant (Bac101) E7 open reading frame were produced in Sf9 insect cells. The variant E7 gene occurred naturally by substitution mutation at the position of 88 nucleotide, resulting serine instead of asparagine. Using E7 specific monoclonal antibody (VD6), both E7 proteins were identified in recombinant baculovirus infected SF9 cells. Radiolabelling and immunoprecipitation analysis revealed that both E7 proteins were phosphoproteins. Immunostaining result showed that E7 proteins were mainly localized in the cytoplasm. Nuclear form of E7 proteins was also detected after a sequential fractionation procedure for removing chromatin structure. Considering that the VD6 recognition site in E7 protein is located within 10 amino acid at the N-terminus, this region appears to be blocked by the nuclear component. Western blot analysis revealed that nuclear form was more abundant than cytoplasmic E7 proteins. Time course immunostaining showed that the primary location of E7 protein was the nucleus and exported to the cytoplasm as proteins were accumulated. These events occurred similarly in both Bac73 and Bac101 infected Sf9 cells, suggesting that these two proteins may have similar biological functions.

• Journal of Microbiology and Biotechnology
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• 제18권1호
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• pp.160-166
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• 2008

A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoding 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with $endo-{\beta}-1,4-D-mannanase$ from Bacillus circulans strain K-1 of the glycoside hydrolase family 5 (GH5). The sequence encoding the mature protein was cloned into the pET-22b(+) vector and expressed in Escherichia coli as a recombinant fusion protein containing an N-terminal hexahistidine sequence. The fusion protein was purified by $Ni^{2+}$ affinity chromatography and its hexahistidine tag cleaved to yield a 31-kDa ${\beta}$-mannanase having a specific activity of 481.55U/mg. The optimal activity of the purified protein, MANB48, was at $58^{\circ}C$ and pH 7.6. The hydrolysis product on substrate locust bean gum included a monosaccharide and mainly oligosaccharides. The recombinant MANB48 may be of potential use in the feed industry.

• 지역사회간호학회지
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• 제9권2호
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• pp.303-312
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• 1998

The purpose of this study was to survey the knowledge level, attitude and practice of nurses toward their work. The subjects of the study were composed of 98 nurses from 3 general hospitals, 1 oriental medical hospital, 2 health centers and several community health posts and schools. Data were collected from May to October, 1998. In data analysis, an SPSS PC program was utilized for descriptions. 1) 16 nurses (16.3%) experienced medical accidents on the 7 nurses(7.1%) 1 time, 6 nurses (6.1%) 2 times, and 3 nurses(3.1%) 3 times. 2) Concerning knowledge of their legal obligations ; the prohibition of telling secrets was .89, the prohibition of reading medical records was .58, the keeping of medical records was 1.0 and the teaching of recuperation was. 79. The total mean score was. 86. Concerning attitude and practice; the prohibition of telling secrets was 81.6%, 63.3%. The prohibition of reading medical records was 61.2%, 60.2%. The keeping of medical records was 98%, 98%. The explanation for treatment, care and test was 91.8%, 66.3%. The teaching for recuperation was 63.3%, 63.3%. 3) Knowledge of their legal responsibilities; 29. 6% of the subjects thought that they should report a medical accident to their headnurse, but 75.5% of the subjects actually reported to the headnurse. 39.8% of the subjects thought that nurses were liable for the faults of nursing aides. The total mean score was .45. 46% of the subjects asked a senior staff's advide on difficult affairs. Nurses obeyed legal obligations when concern ing the protection of a client, but were passive when concerning self protection. Also, headnurses were required as adviser, guide and advocate.

• 한국비블리아학회지
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• 제26권2호
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• pp.269-287
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• 2015

본 연구는 도서관의 성과를 측정하기 위한 방법의 하나로 논리모델의 적용가능성을 논의했다. 사례연구로 연구대상 도서관이 운영하는 어린이와 청소년을 위한 독서프로그램 중의 하나인 독서대학의 성과를 측정하기 위한 논리모델 성과 프레임워크를 개발하였다. 논리모델에 포함된 성과요소 중에서 도서관 데이터를 통해 측정이 가능한 중기성과를 중심으로 성과를 측정하였고 측정결과, 독서대학은 연구대상 도서관이 제시하고 있는 산출평가 방식의 결과물에서 나타나지 않은 성과들을 만들어냈다. 도서대출과 관련하여 독서대학 참여자의 52%는 긍정적인 성과를 경험했다. 또한 프로그램 종료 후 참여자의 독서동아리 가입이 증가했고 다양한 주제분야의 도서를 읽는 경향을 보였다. 그러나 다른 독서관련 프로그램 참여율이나 독서대학의 재참여율은 가시적인 성과를 보이지 않았다. 이러한 연구결과는 논리모델이 도서관 성과연구에 기여할 수 있음을 보여주고 있다.

• Journal of Microbiology and Biotechnology
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• 제18권7호
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• pp.1221-1226
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• 2008

The soft rot bacterium Pectobacterium wasabiae is an economically important pathogen of many crops. A new phytase gene, appA, was cloned from P. wasabiae by degenerate PCR and TAIL-PCR. The open reading frame of appA consisted of 1,302 bp encoding 433 amino acid residues, including 27 residues of a putative signal peptide. The mature protein had a molecular mass of 45 kDa and a theoretical pI of 5.5. The amino acid sequence contained the conserved active site residues RHGXRXP and HDTN of typical histidine acid phosphatases, and showed the highest identity of 48.5% to PhyM from Pseudomonas syringae. The gene fragment encoding the mature phytase was expressed in Escherichia coli BL21 (DE3), and the purified recombinant phytase had a specific activity of 1,072$\pm$47 U/mg for phytate substrate. The optimum pH and temperature for the purified phytase were pH 5.0 and 50$^{\circ}C$, respectively. The $K_m$ value was 0.17 mM, with a $V_{max}$ of 1,714 $\mu$mol/min/mg. This is the first report of the identification and isolation of phytase from Pectobacterium.

• International Journal of Industrial Entomology and Biomaterials
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• 제18권1호
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• pp.33-40
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• 2009

A vitellogenin cDNA was cloned from the bumblebee Bombus ignitus. The cDNA encoding B. ignitus vitellogenin (BiVg) is 5473 bases long with an open reading frame of 1773 amino acid residues. BiVg possesses two consensus (RXXR/S) cleavage sites and has the conserved DGXR and GL/ICG motif near its C-terminus. The deduced amino acid sequence of BiVg cDNA showed significant similarity with hymenopteran Vgs (51% identity to Apis mellifera Vg, and $33{\sim}36%$ to other insect Vgs). The BiVg cDNA was expressed as a 200-kDa polypeptide in baculovirus-infected insect Sf9 cells. Northern and Western blot analyses showed the expression of BiVg in fat bodies of pupae and adults. In queens, the expression of BiVg was first detected in late pupal stage fat bodies, and secreted BiVg was also observed in late pupal stage hemolymph.