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http://dx.doi.org/10.7852/jses.2012.50.2.189

Molecular characterization of diapause-related genes in the bumblebee, Bombus ignitus  

Hwang, Jae-Sam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Park, In-Gyun (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yun, Eun-Young (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Jung, Duck-Oung (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Yoon, Hyung Joo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Lee, Sang Beom (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, Seong-Ryul (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Lee, Joon-Ha (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kim, In-Woo (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Kwon, Young Nam (Department of Agricultural Biology, National Academy of Agricultural Science, RDA)
Publication Information
Journal of Sericultural and Entomological Science / v.50, no.2, 2012 , pp. 189-193 More about this Journal
Abstract
To find diapause-related genes, we were performed by differential hybridization with three types of [${\alpha}-^{32}P$]dCTP-labeled total cDNA probes synthesized from diapause-prepared, diapause-maintained and diapause-activated stage of Bombus ignitus queen. Nine individual cDNA clones were found to be differentially expressed in diapause-maintained and diapause-activated stage. Among these clones, BIDC9(BIDC ; Bombus ignitus differentially expressed clone) was analyzed through full-length sequencing and expression pattern analysis. This clone was specifically expressed in the thorax organ. The effect of Juvenile hormone analog(JHA) and $CO_2$ treatment was examined. JHA treatment induced the expression of BIDC9 cDNA clone abruptly after 4 day of treatment. $CO_2$ treatment induced also the clone after 2 day of treatment. BIDC9 cDNA was identified as Bombus ignitus diapause gene contained an open reading frame of 1376 bp encoding 255 amino acids.
Keywords
Bombus ignitus; Diapause-related gene;
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Times Cited By KSCI : 1  (Citation Analysis)
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