Browse > Article

Gene Cloning, Expression, and Characterization of a Novel ${\beta}$-Mannanase from Bacillus circulans CGMCC 1416  

Li, Yanan (Feed Research Institute, Chinese Academy of Agricultural Sciences)
Yang, Peilong (Feed Research Institute, Chinese Academy of Agricultural Sciences)
Meng, Kun (Feed Research Institute, Chinese Academy of Agricultural Sciences)
Wang, Yaru (Feed Research Institute, Chinese Academy of Agricultural Sciences)
Luo, Huiying (Feed Research Institute, Chinese Academy of Agricultural Sciences)
Wu, Ningfeng (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences)
Fan, Yuliu (Biotechnology Research Institute, Chinese Academy of Agricultural Sciences)
Yao, Bin (Feed Research Institute, Chinese Academy of Agricultural Sciences)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.1, 2008 , pp. 160-166 More about this Journal
Abstract
A DNA fragment containing 2,079 base pairs from Bacillus circulans CGMCC 1416 was cloned using degenerate PCR and inverse PCR. An open reading frame containing 981 bp was identified that encoding 326 amino acids residues, including a putative signal peptide of 31 residues. The deduced amino acid sequence showed the highest identity (68.1%) with $endo-{\beta}-1,4-D-mannanase$ from Bacillus circulans strain K-1 of the glycoside hydrolase family 5 (GH5). The sequence encoding the mature protein was cloned into the pET-22b(+) vector and expressed in Escherichia coli as a recombinant fusion protein containing an N-terminal hexahistidine sequence. The fusion protein was purified by $Ni^{2+}$ affinity chromatography and its hexahistidine tag cleaved to yield a 31-kDa ${\beta}$-mannanase having a specific activity of 481.55U/mg. The optimal activity of the purified protein, MANB48, was at $58^{\circ}C$ and pH 7.6. The hydrolysis product on substrate locust bean gum included a monosaccharide and mainly oligosaccharides. The recombinant MANB48 may be of potential use in the feed industry.
Keywords
${\beta}$-Mannanase; Bacillus circulans; gene expression; characterization;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 8  (Related Records In Web of Science)
연도 인용수 순위
1 Arcand, D. N., D. F. Kluepfel, M. R. Paradis, and F. Shareck. 1993. $\beta$-Mannanase of Streptomyces lividans 66: Cloning and DNA sequence of the manA gene and characterization of the enzyme. Biochem. J. 290: 857-863   DOI
2 Baird, D. S., M. A. Hefford, D. A. Johnson, L. W. Sung, M. Yaguchi, and V. L. Seligy. 1990. The Glu residue in the conserved Asn-Glu-Pro sequence of two highly divergent endo- $\beta$-1,4-glucanases is essential for enzymatic activity. Biochem. Biophys. Res. Commun. 169: 1035-1039   DOI   ScienceOn
3 Guiseppi, A., B. Cami, J. L. Aymeric, G. Ball, and N. Creuzet. 1998. Homology between endoglucanase Z of Erwinia chrysanthemi and endoglucanases of Bacillus subtilis and alkalophilic Bacillus. Mol. Microbiol. 2: 159-164
4 Kesty, C. N. and J. M. Kuehn. 2004. Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles. J. Biol. Chem. 279: 2069-2076   DOI   ScienceOn
5 Lee, J. T., C. A. Bailey, and A. L. Cartwright. 2003. Betamannanase ameliorates viscosity-associated depression of growth in broiler chickens fed guar germ and hull fractions. Poult. Sci. 82: 1925-1931   DOI
6 Puls, J. 1997. Chemistry and biochemistry of hemicelluloses - relationship between hemicellulose structure and enzymes required for hydrolysis. Macromol. Symp. 120: 183-196
7 Wang, Q., D. Tull, A. Meinke, R. N. Gilkes, J. A. R. Warren, R. Aebersold, and G. S. Withers. 1993. $Glu^{280}$ is the nucleophile in the active site of Clostridium thermocellum CelC, a family A endo-${\beta}$-1,4-glucanase. J. Biol. Chem. 268: 14096-14102
8 Yang, P., P. Shi, Y. Wang, Y. Bai, K. Meng, H. Luo, T. Yuan, and B. Yao. 2007. Cloning and overexpression of a Paenibacillus $\beta$- glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. J. Microbiol. Biotechnol. 17: 58-66   과학기술학회마을
9 Omogbenigun, F. O., C. M. Nyachoti, and B. A. Slominski. 2004. Dietary supplementation with multienzyme preparations improves nutrient utilization and growth performance in weaned pigs. J. Anim. Sci. 82: 1053-1061   DOI
10 Leggio, L., L. Parry, N. J. Beeumen, J. Van, M. Claeyssens, M. K. Bhat, and R. W. Pickersgill. 1997. Crystallization and preliminary X-ray analysis of the major endoglucanase from Thermoascus aurantiacus. Acta Crystallogr. 53: 599-604
11 Yosida, S., Y. Sako, and A. Uchida. 1997. Purification, properties, and N-terminal amino acid sequence of guar gum-degrading enzyme from Bacillus circulans K-1. Biosci. Biotech. Biochem. 61: 251-255   DOI   ScienceOn
12 Fraissi, M., N. Y. S. Woo, and J. Noailla-Depeyre. 1981. Distribution pattern of digestive enzyme activities in the intestine of the catfish (Ameiums nebulosus L.) and of the carp (Cyprinus carpio L). Comp. Biochem. Physiol. 70A: 443-446
13 Hilge, M., M. S. Gloor, W. Rypniewski, O. Sauer, D. T. Heightman, W. Zimmermann, K. Winterhalter, and K. Piontek. 1998. High-resolution native and complex structures of thermostable $\beta$-mannanase from Thermomonospora fusca substrate specificity in glycosyl hydrolase family 5. Structure 6: 1433-1444   DOI   ScienceOn
14 Vasina, J. A. and X. F. Baney. 1997. Expression of aggregationprone recombinant proteins at low temperatures: A comparative study of the Escherichia coli cspA and tac promoter systems. Protein Expr. Purif. 9: 211-218   DOI   ScienceOn
15 Yoshida, S., Y. Sako, and A. Uchida. 1998. Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for an enzyme from Bacillus circulans K-1 that degrades guar gum. Biosci. Biotech. Biochem. 62: 514-520   DOI   ScienceOn
16 McCutchen, M. C., D. G. Duffaud, P. Leduc, H. A. R. Peterson, A. Tayal, A. S. Khan, and M. R. Kelly. 1996. Characterization of extremely thermostable enzymatic breakers (${\alpha}$-1,6- galactosidase and ${\beta}$-1,4 mannanase) from the hyperthermophilic bacterium Thermotoga neapolitana 5068 for hydrolysis of guar gum. Biotechnol. Bioeng. 52: 332-339   DOI   ScienceOn
17 Henrissat, B. 1991. A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280: 309-316   DOI
18 Xu, B., D. Sellos, and J. C. Janson. 2002. Cloning and expression in Pichia pastoris of a blue mussel (Mytilus edulis) ${\beta}$-mannanase gene. Eur. J. Biochem. 269: 1753-1760   DOI   ScienceOn
19 Belaich, A., H. P. Fierobe, D. Baty, B. Busetta, C. Bagnara- Tardif, C. Gaudin, and J. P. Belaich. 1992. The catalytic domain of endoglucanase A from Clostridium cellulolyticum: Effects of arginine 79 and histidine 122 mutations on catalysis. J. Bacteriol. 174: 4677-4682   DOI
20 Sumner, J. B. and G. F. Somers. 1949. Dinitrosalicylic acid method for glucose. In: Laboratory Expression in Biological Chemistry. Academic Press, New York
21 Macarron, R., J. V. Beeumen, B. Henrissat, I. Mata, and M. Claeyssens. 1993. Identification of an essential glutamate residue in the active site of endoglucanase III from Trichoderma reesei. FEBS Lett. 316: 137-140   DOI   ScienceOn
22 Ma, Y., Y. Xue, Y. Dou, Z. Xu, W. Tao, and P. Zhou. Characterization and gene cloning of a novel beta-mannanase from alkaliphilic Bacillus sp., N16-5. Extremophiles 8: 447-454   DOI   ScienceOn
23 Stalbrand, H., A. Saloheimo, J. Vehmaanpera, B. Henrissat, and M. Penttila. 1995. Cloning and expression in Saccharomyces cerevisiae of a Trichoderma reesei ${\beta}$-mannanase gene containing a cellulose binding domain. Appl. Environ. Microbiol. 61: 1090-1097
24 Wong, K. K. Y. and J. N. Saddler. 1993. Applications of hemicellulases in the food, feed, and pulp and paper industries, pp. 127-143. In M. P. Coughlan and G. P. Hazlewood (eds.), Hemicellulose and Hemicellulases. Portland Press Ltd., London
25 Buchert, J., J. Salminen, M. Sika-aho, M. Ranua, and L. Viikari. 1993. The role of Trichoderma reesei xylanase and mannanase in the treatment of softwood kraft pulp prior to bleaching. Holzforschung 47: 473-478   DOI   ScienceOn
26 Halstead, J. R., M. P. Fransen, Y. R. Eberhart, J. A. Park, J. H. Gilbert, and P. G. Hazlewood. 2000. ${\alpha}$-Galactosidase A from Pseudomonas fluorescens subsp. cellulosa: Cloning, high level expression and its role in galactomannan hydrolysis. FEMS Microbiol. Lett. 192: 197-203
27 Wang, S. Y., S. J. Wu, G. Thottappilly, R. Locy, and N. Singh. 2001. Molecular cloning and structural analysis of the gene encoding Bacillus cereus exochitinase Chi36. J. Biosci. Bioeng. 92: 59-66   DOI   ScienceOn
28 Kuhad, R. C., A. Singh, and K. E. Eriksson. 1997. Microorganisms and enzymes involved in the degradation of plant fiber cell walls. Adv. Biochem. Eng. Biotechnol. 57: 45-125
29 Silver, J. and V. Keerikatte. 1989. Novel use of polymerase chain-reaction to amplify cellular DNA adjacent to an integrated provirus. J. Virol. 63: 1924-1928
30 Dutta, S., J. K. Bradford, and J. D. Nevins. 1997. Endo-betamannanase activity present in cell wall extracts of lettuce endosperm prior to radicle emergence. Plant Physiol. 113: 155-161   DOI
31 Suurnakki, A., M. Tenkanen, J. Buchert, and L. Viikari. 1997. Hemicellulases in the bleaching of chemical pulps. Adv. Biochem. Eng. Biotechnol. 57: 261-287
32 Tanaka, M., M. Taniguchi, R. Matsuno, and T. Kamikubo. 1988. Cellulases from Eupenicillium javanicum. Methods Enzymol. 160: 251-259   DOI
33 Bewley, D. J., A. R. Burton, Y. Morohashi, and B. G. Fincher. 1997. Molecular cloning of a cDNA encoding a (1-4)-$\beta$-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum). Planta 203: 454-459   DOI   ScienceOn
34 Puchar, V., M. Vrsanska, P. Svoboda, J. Pohl, B. Z. Ogel, and P. Biely. 2004. Purification and characterization of two forms of endo-beta-1,4-mannanase from a thermotolerant fungus, Aspergillus fumigatus IMI 385708 (formerly Thermomyces lanuginosus IMI 158749). Biochim. Biophys. Acta 1674: 239-250   DOI   ScienceOn
35 Araujo, A. and O. P. Ward. 1990. Hemicellulases of Bacillus species: Preliminary comparative studies on production and properties of mannanases and galactanases. J. Appl. Bacteriol. 68: 253-261   DOI