Browse > Article

Molecular Cloning and Characterization of a Vitellogenin of the Bumblebee Bombus ignitus  

Lee, Kyung-Yong (College of Natural Resources and Life Science, Dong-A University)
Yoon, Hyung-Joo (Department of Agricultural Biology, National Academy of Agricultural Science)
Lee, Sang-Beom (Department of Agricultural Biology, National Academy of Agricultural Science)
Park, In-Gyun (Department of Agricultural Biology, National Academy of Agricultural Science)
Sohn, Hung-Dae (College of Natural Resources and Life Science, Dong-A University)
Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.18, no.1, 2009 , pp. 33-40 More about this Journal
Abstract
A vitellogenin cDNA was cloned from the bumblebee Bombus ignitus. The cDNA encoding B. ignitus vitellogenin (BiVg) is 5473 bases long with an open reading frame of 1773 amino acid residues. BiVg possesses two consensus (RXXR/S) cleavage sites and has the conserved DGXR and GL/ICG motif near its C-terminus. The deduced amino acid sequence of BiVg cDNA showed significant similarity with hymenopteran Vgs (51% identity to Apis mellifera Vg, and $33{\sim}36%$ to other insect Vgs). The BiVg cDNA was expressed as a 200-kDa polypeptide in baculovirus-infected insect Sf9 cells. Northern and Western blot analyses showed the expression of BiVg in fat bodies of pupae and adults. In queens, the expression of BiVg was first detected in late pupal stage fat bodies, and secreted BiVg was also observed in late pupal stage hemolymph.
Keywords
Bombus ignitus; Bumblebee; Hemolymph; Insect; Vitellogenin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Choi YS, Choo YM, Lee KS, Yoon HJ, Kim I, Je YH, Sohn HD, Jin BR (2008) Cloning and expression profiling of four antibacterial peptide genes from the bumblebee Bombus ignitus. Comp Biochem Physiol B 150, 141-146   DOI   ScienceOn
2 Comas D, Piulachs MD, Belles X (2000) The vitellogenin of Blattella germanica (L.) (Dictyoptera Blattellidae): nucleotide sequence of the cDNA and analysis of the protein structure. Arch Insect Biochem Physiol 45, 1-11   DOI   ScienceOn
3 Hiremath S, Lehtoma K (1997b) Structure of the gypsy moth vitellogenin gene. Arch Insect Biochem Physiol 36, 154-164   DOI   ScienceOn
4 Romans P, Tu Z, Ke Z, Hagedorn HH (1995) Analysis of a vitellogenin gene of the mosquito Aedes aegypti and comparisons to vitellogenins from other organisms. Insect Biochem Mol Biol 25, 939-958   DOI   ScienceOn
5 Tufail M, Lee JM, Hatakeyama M, Oishi K, Takeda M (2000) Cloning of vitellogenin cDNA of the American cockroach, Periplaneta americana (Dictyoptera), and its structural and expression analyses. Arch Insect Biochem Physiol 45, 37-46   DOI   ScienceOn
6 Yano K, Sakurai MT, Watabe S, Izumi S, Tomino S (1994b). Structure and expression of mRNA for vitellogenin in Bombyx mori. Biochim Biophys Acta 1218, 1-10   DOI   PUBMED   ScienceOn
7 Yoon HJ, Kim SE, Kim YS (2002) Temperature and humidity favorable for colony development of the indoor-reared bumblebee, Bombus ignitus. Appl Entomol Zool 37, 419-423   DOI   ScienceOn
8 Barchuk AR, Bitondi MMG, Sim\tilde{o}es ZLP (2002) Effects of juvenile hormone and ecdysone on the timing of vitellogenin appearance in hemolymph of queen and worker pupae of Apis mellifera. J Insect Sci 2, 1-8
9 Hiremath S, Lehtoma K (1997a) Complete nucleotide sequence of the vitellogenin mRNA from the gypsy moth: novel arrangement of the subunit encoding regions. Insect Biochem Mol Biol 27, 27-35   DOI   ScienceOn
10 Nose Y, Lee JM, Ueno T, Hatakeyama M, Oishi K (1997) Cloning of cDNA for vitellogenin of the parasitoid wasp Pimpla nipponica (Hymenoptera: Apocrita: Ichneumonidae): vitellogenin primary structure and evolutionary considerations. Insect Biochem Mol Biol 27, 1047-1056   DOI   ScienceOn
11 Liu CL, Kajiura Z, Shiomi K, Takei R, Nakagaki M (2001) Purification and cDNA sequencing of vitellogenin of the wild silkworm, Antheraea pernyi. J Insect Biotech Sericol 70, 95-104
12 Choi YS, Lee KS, Yoon HJ, Kim I, Sohn HD, Jin BR (2006) Bombus ignitus Cu,Zn superoxide dismutase (SOD1): cDNA cloning, gene structure, and up-regulation in response to paraquat, temperature stress, or lipopolysaccharide stimulation. Comp Biochem Physiol B 144, 365-371   DOI   ScienceOn
13 Lee JM, Hatakeyama M, Oishi K (2000a) A simple and rapid method for cloning insect vitellogenin cDNAs. Insect Biochem Mol Biol 30, 189-194   DOI   ScienceOn
14 Yoon HJ, Kim SE, Kim YS, Lee SB (2004) Colony developmental characteristics of the bumblebee queen, Bombus ignitus by the first oviposition day. Int J Indust Entomol 8, 139-143
15 Bownes M (1986) Expression of gene coding for vitellogenin (yolk protein). Annu Rev Entomol 31, 507-531   DOI   ScienceOn
16 Sappington TW, Raikhel AS (1998) Molecular characteristics of insect vitellogenins and vitellogenin receptors. Insect Biochem Mol Biol 28, 277-300   DOI   ScienceOn
17 Trewitt PM, Heilmann LJ, Degrugillier SS, Kumaran AK (1992) The boll weevil vitellogenin gene: nucleotide sequence, structure, and evolutionary relationship to nematode and vertebrate vitellogenin genes. J Mol Evol 34, 478-492   DOI   PUBMED
18 Raikhel AS, Dhadialla TS (1992) Accumulation of yolk proteins in insect oocytes. Annu Rev Entomol 37, 217-251   DOI   ScienceOn
19 Chen JS, Sappington TW, Raikhel AS (1997) Extensive sequence conservation among insect, nematode and vertebrate vitellogenins reveals ancient common ancestry. J Mol Evol 44, 440-451   DOI   ScienceOn
20 Engels W, Kaatz H, Zillikens A, Paulino Simoes ZL, Trube A, Braun R, Dittruch F (1990) Honeybee reproduction: Vitellogenin and caste-specific regulation of fertility; in Advances in Invertebrate Reproduction 5 (Hoshi M, Yamashita O eds), pp. 495-502, Elsevier Ltd., Amsterdam
21 Yano K, Sakurai MT, Izumi S, Tomino S (1994a) Vitellogenin gene of the silkworm Bombyx mori: structure and sex-dependent expression. FEBS Letters 256, 207-211   DOI   ScienceOn
22 Piulachs MD, Guidugli KR, Barchuk AR, Cruz J, Simoes ZLP, Belles X (2003) The vitellogenin of the honey bee Apis mellifera: structural analysis of the cDNA and expression studies. Insect Biochem Mol Biol 33, 49-465   DOI   ScienceOn
23 Lee JM, Nishimori Y, Hatakeyama M, Bae TW, Oishi K (2000b) Vitellogenin of the cicada Graptopsaltria nigrofuscata (Homoptera): analysis of its primary structure. Insect Biochem Mol Biol 30, 1-7   DOI   ScienceOn
24 Byrne BM, Grube M, Ab G (1989) The evolution of egg yolk proteins. Prog Biophys Mol Biol 53, 33-69   DOI   ScienceOn
25 Tufail M, Takeda M (2002) Vitellogenin of the cockroach, Leucophaea maderae: nucleotide sequence, structure and analysis of processing in the fat body and oocytes. Insect Biochem Mol Biol 32, 117-134   DOI   ScienceOn
26 Hagedorn HH, Maddison DR, Tu Z (1998) The evolution of vitellogenins, cyclorrhaphan yolk proteins and related molecules. Adv Insect Physiol 27, 335-384   DOI
27 Giorgi F, Bradley JT, Nordin JH (1999) Differential vitellin polypeptide processing in insect embryos. Micron 30, 579-596   DOI   ScienceOn
28 Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685   DOI   PUBMED   ScienceOn
29 Tufail M, Bembenek J, Elgendy AM, Takeda M (2007) Evidence for two vitellogenin-related genes in Leucophaea maderae: the protein primary structure and its processing. Arch Insect Biochem Physiol 66, 190-203   DOI   ScienceOn
30 Tufail M, Hatakeyama M, Takeda M (2001) Molecular evidence for two vitellogenin genes and processing of vitellogenins in the American cockroach, Periplaneta americana. Arch Insect Biochem Physiol 48, 72-80   DOI   ScienceOn
31 Trenczek TA, Engels W (1986) Occurring of vitellogenin in drone honeybees. Int J Invertebr Repr Dev 10, 307-311   DOI
32 Chen JS, Cho WL, Raikhel AS (1994) Analysis of mosquito vitellogenin cDNA similarity with vertebrate phosvitins and arthropod serum proteins. J Mol Biol 237, 641-647   DOI   ScienceOn
33 Donnell DM (2004) Vitellogenin of the parasitoid wasp Encarsia formosa (Hymenoptera: Aphelinidae): gene organization and differential use by members of the genus. Insect Biochem Mol Biol 34, 951-961   DOI   PUBMED   ScienceOn
34 Je YH, Chang JH, Choi JY, Roh JY, Jin BR, O’Reilly DR, Kang SK (2001) A defective viral genome maintained in Escherichia coli for the generation of baculovirus expression vectors. Biotechnol Lett 23, 575-582   DOI   ScienceOn
35 Hirai M, Watanabe D, Kiyota A, Chinzei Y (1998) Nucleotide sequence of vitellogenin mRNA in the bean bug Riptortus clavatus: analysis of processing in the fat body and ovary. Insect Biochem Mol Biol 28, 537-547   DOI   ScienceOn
36 Kageyama Y, Kinoshita T, Umesono Y, Hatakeyama M, Oishi K (1994) Cloning of cDNA for vitellogenin of Athalia rosae (Hymenoptera) and characterization of the vitellogenin gene expression. Insect Biochem Mol Biol 24, 599-605   DOI   ScienceOn
37 Swevers L, Raikhel AS, Sappington TW, Shirk P, Latrou K (2005) Vitellogenesis and post-vitellogenic maturation of the insect ovarian follicle; in Comprehensive Molecular Insect Science (Gilbert, L. I., Iatrou, K. and Gill, S. S. eds), pp. 87-155, Elsevier Ltd., Oxford