• Journal of Microbiology and Biotechnology
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• 제22권2호
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• pp.248-255
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• 2012

In order to develop a protocol to quantify cyanobacteria and Microcystis simultaneously, the primers and probe were designed from the conserved regions of 16S rRNA gene sequences of cyanobacteria and Microcystis, respectively. Probe match analysis of the Ribosomal Database Project showed that the primers matched with over 97% of cyanobacterial 16S rRNA genes, indicating these can be used to amplify cyanobacteria specifically. The TaqMan probe, which is located between two primers, matched with 98.2% of sequences in genus GpXI, in which most Microcystis strains are included. The numbers of cyanobacterial genes were estimated with the emission of SYBR Green from the amplicons with two primers, whereas those of Microcystis spp. were measured from the fluorescence of CAL Fluor Gold 540 emitted by exonuclease activity of Taq DNA polymerase in amplification. It is expected that this method enhances the accuracy and reduces the time to count cyanobacteria and potential toxigenic Microcystis spp. in aquatic environmental samples.

• 한국식품과학회지
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• 제53권6호
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• pp.682-687
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• 2021

The purpose of this study was to validate a high-performance liquid chromatography (HPLC) method for the quantitative analysis of quercetin in various foods. The method was based on HPLC-UV (360 nm). The method was validated using candy, beverage, and sausage which were tested for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. Matrix-matched calibration was also applied. The calibration curves (0.5-50 mg/L) showed good linearity (r²≥0.9998). LOD and LOQ ranged from 0.15 to 0.31 mg/kg and from 0.44 to 0.93 mg/kg, respectively. The average accuracy and precision at 0.5, 2.5, and 10 mg/kg ranged from 84.3 to 102.0% and 0.7 to 3.0 relative standard deviation (RSD%), respectively. This study confirmed the applicability of the proposed method by applying it to commercial products, such as teas and beverages. Thus, the proposed analytical method is suitable for quantifying quercetin in various foods.

• 교육녹색환경연구
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• 제18권2호
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• pp.1-11
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• 2019

본 연구는 지속적으로 증가가 예상되는 폐교시설의 효율적인 활용을 위하여 활용지표의 개발을 통해 폐교 활용 시 객관적인 의사결정을 위한 목적을 가진다. 연구 단계는 크게 폐교 활용 예비지표 도출, 빅데이터를 활용한 최종지표 도출, 지표의 정량화 단계로 구분하여 진행하였으며, 최종적으로 지표를 정량화함으로써 객관화하였다. 향후 지표를 기준으로 시설에 적용 및 검증하고자 한다. 본 연구는 지금까지 폐교시설의 활용을 위한 계획 및 연구에 있어서 시도되지 않았던 빅데이터 분석기법을 적용한 것에 그 의의가 있다.

• 분석과학
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• 제35권5호
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• pp.218-227
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• 2022

A simple and fast throughput flow injection (FI) system with a merging-zone technique was designed to determine ferrous and ferric in an aqueous solution. The method is based on the direct reaction of ferrous with a Bathophenanthroline reagent (Bphen) in acidic media. The forming red complex absorbs light at 533 nm. All conditions of the flow injection system were investigated. The analytical curve of ferrous was linear in the range of 0.07 to 4 mg/L with an r² value of 0.9968. The detection and quantification limits were 0.02 and 0.04 mg/L, respectively. The molar absorptivity and Sandell's sensitivity were 4.0577 × 10⁶ L/mol cm and 25 × 10^-5 ㎍/cm², respectively. The homemade valve was low-cost with high repeatability (n = 7) at an RSD of 1.26 % and zero dead volume. The values of the dispersion coefficient were 2.318, 2.022, and 1.636 for the concentrations of 0.2, 1, and 3 mg/L, respectively. The analysis throughput of the designed flow injection unit was 57 sample per hour.

• 분석과학
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• 제34권4호
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• pp.153-159
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• 2021

In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 ㎍/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 ㎍/mL and 0.08-0.26 ㎍/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.

• Nuclear Engineering and Technology
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• 제55권1호
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• pp.119-130
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• 2023

Among the various elements of probabilistic safety assessment (PSA), human failure events (HFEs) and their dependencies are major contributors to the quantification of risk of a nuclear power plant. Currently, the dependency among HFEs is reflected using a post-processing method in PSA, wherein several drawbacks, such as limited propagation of minimal cutsets through the fault tree and improper truncation of minimal cutsets exist. In this paper, we propose a method to model the HFE dependency directly in a fault tree using the if-then-else logic. The proposed method proved to be equivalent to the conventional post-processing method while addressing the drawbacks of the latter. We also developed a software tool to facilitate the implementation of the proposed method considering the need for modeling the dependency between multiple HFEs. We applied the proposed method to a specific case to demonstrate the drawbacks of the conventional post-processing method and the advantages of the proposed method. When applied appropriately under specific conditions, the direct fault-tree modeling of HFE dependency enhances the accuracy of the risk quantification and facilitates the analysis of minimal cutsets.

• 대한교통학회지
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• 제27권2호
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• pp.117-131
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• 2009

고속도로이용자가 주행할 때 도로기하구조를 정확하게 파악하지 못하면, 도로이용자는 자신의 감각과 인식에 의존해 판단을 내리게 되기 때문에 고속도로의 주행성을 평가할 때 설계자와는 다른 판단을 내리게 될 수도 있다. 따라서 도로이용자의 기대심리를 만족시키면서 안전성, 쾌적성 등을 확보하기 위해서는 정확한 주행속도를 예측할 필요가 있다. 또한, 고속도로의 기하구조적인 주행성뿐만 아니라 도로이용자가 이러한 구성요소의 조화에 의해 느끼는 감성적 측면에서의 주행환경만족도에 대한 인식의 변화를 파악할 필요가 있다. 이에 본 연구에서는 주행속도 예측모형 구축하기 위해 6개 고속도로(경부, 서해안, 영동, 남해, 중앙, 호남)를 대상으로 주행속도에 영향을 미치는 요인을 크게 선형요소, 교통운영특성 요소로 분류하였다. 주행속도에 영향을 미치는 요인을 대상으로 다중회귀를 이용한 주행속도예측모형 구축 및 검증 결과, 통계적으로 모두 적합한 것으로 나타났다. 또한, 본 연구에서는 고속도로 이용자를 대상으로 개별면접 설문조사를 통해 수집된 주행환경만족도 이미지 데이터를 바탕으로 수량화 제II류 및 주성분분석을 실시하여 고속도로 주행환경만족도에 대한 영향 요인을 규명하였다. 또한 수량화 제I류 및 구조방정식을 이용하여 사회인식 및 고속도로 이용자의 실제인식의 변화가 고려된 고속도로의 주행환경만족도 모형을 구축하였다. 본 연구에서 구축된 모형을 통해 주행속도와 같은 정량적인 요인뿐만 아니라 도로이용자가 느끼는 고속도로의 주행환경만족도와 같은 정성적인 요인에 대한 결과도 제시할 수 있어서 도로이용자가 느끼는 변화된 교통 환경과 인식여부가 반영된 고속도로의 주행성을 평가할 수 있다는 데에 그 의의가 있다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8553-8557
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• 2016

Background: Accurate dose assessment and correct identification of irradiated from non-irradiated people are goals of biological dosimetry in radiation accidents. Objectives: Changes in the FDXR and the RAD51 gene expression (GE) levels were here analyzed in response to total body exposure (TBE) to a 6 MV x-ray beam in rats. We determined the accuracy for absolute quantification of GE to predict the dose at 24 hours. Materials and Methods: For this in vivo experimental study, using simple randomized sampling, peripheral blood samples were collected from a total of 20 Wistar rats at 24 hours following exposure of total body to 6 MV X-ray beam energy with doses (0.2, 0.5, 2 and 4 Gy) for TBE in Linac Varian 2100C/D (Varian, USA) in Golestan Hospital, in Ahvaz, Iran. Also, 9 rats was irradiated with a 6MV X-ray beam at doses of 1, 2, 3 Gy in 6MV energy as a validation group. A sham group was also included. After RNA extraction and DNA synthesis, GE changes were measured by the QRT-PCR technique and an absolute quantification strategy by taqman methodology in peripheral blood from rats. ROC analysis was used to distinguish irradiated from non-irradiated samples (qualitative dose assessment) at a dose of 2 Gy. Results: The best fits for mean of responses were polynomial equations with a R2 of 0.98 and 0.90 (for FDXR and RAD51 dose response curves, respectively). Dose response of the FDXR gene produced a better mean dose estimation of irradiated "validation" samples compared to the RAD51 gene at doses of 1, 2 and 3 Gy. FDXR gene expression separated the irradiated rats from controls with a sensitivity, specificity and accuracy of 87.5%, 83.5% and 81.3%, respectively, 24 hours after dose of 2 Gy. These values were significantly (p<0.05) higher than the 75%, 75% and 75%, respectively, obtained using gene expression of RAD51 analysis at a dose of 2 Gy. Conclusions: Collectively, these data suggest that absolute quantification by gel purified quantitative RT-PCR can be used to measure the mRNA copies for GE biodosimetry studies at comparable accuracy to similar methods. In the case of TBE with 6MV energy, FDXR gene expression analysis is more precise than that with RAD51 for quantitative and qualitative dose assessment.

• 한국도로학회논문집
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• 제12권2호
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• pp.51-61
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• 2010

경제성장과 함께 삶의 질이 향상됨에 따라 도로 계획 및 설계분야에서도 기능 중심의 설계에서 이용자들의 시각 및 심리적 특성인 감성을 고려한 경관 중심의 설계로 변화하고 있다. 이에 본 연구에서는 다양하게 조사된 감성형용사들을 개인의 감성인지 특성 차이를 최소화 하면서 객관적이고 합리적인 추출 방법을 제안하고자 한다. 먼저 어의구별법(Semantic Differential)의 5점 척도에 의해 조사된 감성형용사를 바탕으로 기존 연구에서 사용한 요인분석과 본 연구에서 제안하는 판별분석을 통해 감성형용사를 추출하고 상관 분석을 통해 대표적인 감성형용사를 추출한 후 수량화 I 모형을 구축하였다. 그리고 구축된 수량화 모형을 이용하여 만족도를 예측한 후 실제 관측 만족도와의 쌍체비교 검증을 통하여 판별 및 상관분석에 의한 방법이 실제 관측 만족도와 가장 유사한 것으로 분석되어 연속적인 도로경관 설계시 판별 상관분석에 의해 적용가능한 감성요인을 파악하는 것이 적합한 방법임을 확인할 수 있었다.

• 한국문헌정보학회지
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• 제8권
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• pp.183-207
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• 1981

Library standards have been the source of considerable controversy, whereas many problems are involved in developing stardard for university library collections. For evaluation purposes, standards should be precise, quantifiable and measurable. In the United States, however, standards for academic libraries are limited to qualitative statements and principles. Quantitative standards, when given, are ususally related to the number of population in the institution being served, or the prescribed quantitative objectives are often arbitrarily formulated by value judgements. The study in this paper attempts to explain the problems involved in developing quantitative standard for academic library collections. Two problems facing in the formulation of the optimal size of collection are identified. One is the theoretically faulty concept of adequacy of collection to meet the situations of diversity of university libraies, and the other is the difficulties in quantification and measurement, along with the lack of concept of adequacy of collection. However, quantification of adequate size of collection is proved to be useful on the pratical level, even though not valid theoretically. ACRL, Clapp/Jordan and Voigt developed formulas or models for setting the optimal size of a library collection for any particular university library. The main purpose of this study is the analysis of the above formulas. ACRL standard was drawn from obervation and analysis of statistcs in leading library collections. In academic field, this judgement appears to have been based on the assumption that a high-grade institution would be apt to have a good library collection. This study criticizes ACRL standard for its failure to include some determinants of measurements, and points out the limitations of the standard. In contrast. Clapp/Jordan developed a formula rather scientifically based upon bibliographical sources. This is similarly empirical but has the advantage of bringing into play the elements which make universities diverse in nature. Both ACRL and Clapp/Jordan formulas share two major defects. (1) the specific subject needs of the collection are not indiacted directly, and (2) percentage rate of growth is an indicator in measuring the potential utility of a collection. Thus both formulas failed to provide a basis for meaningful evaluation. Voigt further developed a model for determining acquisition rates for currently published materials based on bibliographic technique. Voigt model encourages experimentation with different programs and different allocations of input resources, designed to meet the needs of the library's particular population. Standard for university library collections can be formulated in terms of input(traditional indicator), or additionally, in terms of output(cost-effectiveness). Cost effectiveness is expressed as user satisfaction, ability to provide wanted materials within a reasonable time period. Thus simple quantitative method does not cover all the situations of diversity of university library collections, nor measures the effectiveness of collections. Valid standard could not be established without further research.