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http://dx.doi.org/10.5806/AST.2021.34.4.153

Optimization of an extraction method for the simultaneous quantification of six active compounds in the aril part of Orostachys japonicus using HPLC-UV  

Gao, Dan (College of Pharmacy, Chungnam National University)
Kim, Jin Hyeok (College of Pharmacy, Chungnam National University)
Cho, Chong Woon (College of Pharmacy, Chungnam National University)
Yang, Seo Young (Department of Pharmaceutical Engineering, Sangji University)
Kim, Young Ho (College of Pharmacy, Chungnam National University)
Kim, Hyung Min (College of Pharmacy, Chungnam National University)
Kang, Jong Seong (College of Pharmacy, Chungnam National University)
Publication Information
Analytical Science and Technology / v.34, no.4, 2021 , pp. 153-159 More about this Journal
Abstract
In this study, we describe the development of a new high-performance liquid chromatography (HPLC) method for the simultaneous analysis of six bioactive compounds (including gallic acid, epicatechin 3-gallate, quercitrin, afzelin, quercetin, and kaempferol) from Orostachys japonicus. The extraction method was investigated and optimization of the extraction time (min), solvent composition (%), and solvent to material ratio were conducted. As a result, 30 min extraction with 50% methanol and 40:1 mL/g of solvent: material ratio achieved the highest extraction efficiency with a yield of 3.32 mg/g. Furthermore, the developed HPLC method was validated and the correlation coefficient (R) values were within the satisfactory range of 0.9995-0.9999 over the linearity range of 1.53-417 ㎍/mL. The limit of detection and limit of quantification for the six active components were between 0.03-0.08 ㎍/mL and 0.08-0.26 ㎍/mL, respectively. With these newly optimized and developed methods, four batches of O. japonicus were analyzed to confirm the high extraction efficiency of the method and the feasibility of an application.
Keywords
HPLC; O. Japonicus; quality control; ultrasonic-assisted extraction; method validation;
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