• 한국식물병리학회지
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• 제14권6호
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• pp.559-562
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• 1998

Odontoglossum ringspot virus (ORSV) was finally purified from ORSV-infected orchid plants by diethylaminoethyl (DEAE) cellulose anion exchange column chromatography. The virus was reliably eluted by potassium chloride at the concentration from 0.1 M to 0.13 M. Partial purification was done by solubilization with Triton X-100 (allkylphenoxypolyethoxy ethanol) and precipitation with polyethylene glycol (PEG; MW 8,000). The finally purified ORSV represented one distinct homogeneous band and the molecular weight of its capsid protein was about 17,500 Dalton in electrophoretic analysis. Electron microscopy showed not only intact particles ranged from 280 nm to 340 nm in length, but also segmented particles that final 140 nm to 220 nm and even disks. Enzyme-linked immunosorbent assay (ELISA) showed that final yield was 12 mg/100 g of the infected leaves. Bioassay demonstrated that the purified ORSV had the normal infectivity to orchid plants and Nicotiana glutionsa. Based on these data, anion exchange column chromatography could be efficiently applied to the purification of ORSV and other viruses similar to ORSV.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 추계학술대회논문초록집
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• pp.336.1-336
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• 2002

Recently, mixers are being widely used in the water purification plant in order to increase the filtration efficiency. The cause of an excessive vibration of a base structure to which the mixers are supported, has been evaluated through modal analysis on the mixer shaft and vibration measurements during operation. The fundamental natural frequency of the mixer shaft is found to be around 1.8 Hz and the main vibratory frequency around 30 Hz. (omitted)

• 한국식물학회:학술대회논문집
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• 한국식물학회 2002년도 춘계학술발표대회:발표눈문요지록
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• pp.88-88
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• 2002

• 융합신호처리학회논문지
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• 제19권4호
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• pp.167-173
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• 2018

빛이 부족하고 공기 순환이 원활하지 않은 실내에서 식물의 지상부와 지하부를 활용한 공기 정화 시스템을 제안한다. 제안된 시스템은 식물의 공기정화 효과를 극대화하고자 지상부는 청색과 녹색의 LED를 조합한 혼합광과 자연광에 가까운 고연색 LED를 각각 조사하고 지하부의 토양을 필터로 활용하고자 팬을 이용하여 강제 순환하였다. 광원과 팬이 결합된 실내공기정화 시스템이 식물만 배치된 경우보다 오염된 공기를 단시간에 가장 많이 제거하였다. 실내 공기정화 시스템의 혼합광과 고연색광 두 가지의 경우에서 식물만 배치된 경우보다 미세먼지는 각각 14%, 14.2%, TVOC는 7.5%, 9.4% 로 더 크게 감소하였다. 팬을 15분 동안만 가동한 실험에서도 TVOC가 97.8%로 크게 감소하였다. 식물의 광합성과 토양을 필터로 활용하는 것이 오염된 공기를 짧은 시간에 정화시킬 수 있었고 팬의 일시적인 가동으로도 지속적인 가동의 효과를 얻었다.

• The Plant Pathology Journal
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• 제20권2호
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• pp.135-141
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• 2004

A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

• KSBB Journal
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• 제28권3호
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• pp.208-212
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• 2013

분무열분해 공정에 의해 물리적 특성이 다른 네 종류의 메조 다공성 실리카를 제조하여 식물세포배양 유래 항암물질 paclitaxel 정제에 사용하였다. 실리카 흡착제의 물리적 특성에서 표면적과 기공부피 보다는 기공크기 (기공지름)이 흡착제 처리 효과에 많은 영향을 미침을 알 수 있었다. 특히 적절한 기공지름 (~9.07 nm)에서 가장 높은 순도 (~46.1%)와 수율 (~82.3%)의 paclitaxel을 얻을 수 있었다. 이러한 불순물 (타르 및 왁스 성분 포함) 제거 효과는 흡착제 처리 후 흡착제를 메탄올로 세척한 시료의 HPLC 분석 결과와 흡착제에 붙은 유기물의 TGA 정량 분석 결과로도 확인할 수 있었다.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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• pp.317-320
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• 1997

In the water purification plant, chemicals are injected for quick purification of raw water. It is clear that the amount of chemicals intrinsically depends on the water quality such as turbidity, temperature, pH and alkalinity etc. However, the process of chemical reaction to improve water quality by the chemicals is not yet fully clarified nor quantified. The feedback signal in the process of coagulant dosage, which should be measured (through the sensor of the plant) to compute the appropriate amount of chemicals, is also not available. Most traditional methods focus on judging the conditions of purifying reaction and determine the amounts of chemicals through manual operation of field experts or jar-test results. This paper presents the method of deriving the optimum dosing rate of coagulant, PAC(Polymerized Aluminium Chloride) for coagulant dosing process in water purification system. A neural network model is developed for coagulant dosing and purifying process. The optimum coagulant dosing rate can be derived the neural network model. Conventionally, four input variables (turbidity, temperature, pH, alkalinity of raw water) are known to be related to the process, while considering the relationships to the reaction of coagulation and flocculation. Also, the turbidity in flocculator is regarded as a new input variable. And the genetic algorithm is utilized to identify the neural network structure. The ability of the proposed scheme validated through the field test is proved to be of considerable practical value.

• 조명전기설비학회논문지
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• 제29권3호
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• pp.54-60
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• 2015

There are 5 purification plants with the adopted advanced water purification treatment process in Korea. Annual operating costs were 8,990 million won including purchase cost of oxygen and power usage charges. We need research to optimize, in the future, when considering the direction of domestic water treatment continues to adopt advanced water treatment process. In this paper, calculate the optimal operating costs by injected the oxygen gas, used power cost. approximately 25% of the operating costs can be reduced when injected the ozone gas is 1.0ppm than 2.0ppm, the necessary amount of oxygen is increased then power is lower. so operating costs are decided according to oxygen costs. On the other hand, high ozone concentration 2.0ppm, the necessary power is increased then amount of oxygen is lower. Therefore, in the case of G purification plant, the controlling factor of the input ozone concentration 2ppm, PID control operation by setting the concentration of over 10Wt% is efficient. The installed capacity is the more little the more better when considering on Ozone injection rate in the process of water treatment.

• Journal of Ginseng Research
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• 제44권6호
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• pp.784-789
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• 2020

Background: The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb₃ from ginseng extracts is limited by the co-existence of its isomer Rb₂. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb₃ from a mixture of isomers. Methods: To isolate Rb₃ from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb₂ into ginsenoside Rd. Ginsenoside Rb₃ was then efficiently separated from the mixture using a traditional chromatographic method. Results: Chromatographic purification of Rb₃ was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb₃ can be used in further pharmaceutical studies. Conclusions: Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb₃. This method can also be applied to purify other isomeric glycoconjugates in mixtures.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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• pp.189-189
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• 2004