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http://dx.doi.org/10.5423/PPJ.2004.20.2.135

Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro (Colocasia esculenta Schott) in Korea  

Kim, Min-Kyu (Division of Biological Environment, Kangwon National University)
Kwon, Soon-Bae (Gangwon Province Agricultural Research and Extension Servic)
Yoon, Ju-Yeon (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University)
Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University)
Heo, Su-Jeong (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University)
Hong, Jeong-Ki (Gangwon Province Agricultural Research and Extension Servic)
Kim, Kyung-Hee (Gangwon Province Agricultural Research and Extension Servic)
Park, Jang-Kyung (Division of Biological Environment, Kangwon National University)
Publication Information
The Plant Pathology Journal / v.20, no.2, 2004 , pp. 135-141 More about this Journal
Abstract
A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.
Keywords
Colocasia esculenta Schott; Dasheen mosaic virus; Zantedeschia mosaic virus;
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