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http://dx.doi.org/10.1016/j.jgr.2019.08.003

Novel enzymatic elimination method for the chromatographic purification of ginsenoside Rb3 in an isomeric mixture  

Cui, Chang-Hao (The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University)
Fu, Yaoyao (The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, Jiangsu Normal University)
Jeon, Byeong-Min (Department of Biological Sciences, Korea Advanced Institute of Science and Technology)
Kim, Sun-Chang (Intelligent Synthetic Biology Center)
Im, Wan-Taek (Department of Biological Sciences, Hankyong National University)
Publication Information
Journal of Ginseng Research / v.44, no.6, 2020 , pp. 784-789 More about this Journal
Abstract
Background: The separation of isomeric compounds from a mixture is a recurring problem in chemistry and phytochemistry research. The purification of pharmacologically active ginsenoside Rb3 from ginseng extracts is limited by the co-existence of its isomer Rb2. The aim of the present study was to develop an enzymatic elimination-combined purification method to obtain pure Rb3 from a mixture of isomers. Methods: To isolate Rb3 from the isomeric mixture, a simple enzymatic selective elimination method was used. A ginsenoside-transforming glycoside hydrolase (Bgp2) was employed to selectively hydrolyze Rb2 into ginsenoside Rd. Ginsenoside Rb3 was then efficiently separated from the mixture using a traditional chromatographic method. Results: Chromatographic purification of Rb3 was achieved using this novel enzymatic elimination-combined method, with 58.6-times higher yield and 13.1% less time than those of the traditional chromatographic method, with a lower minimum column length for purification. The novelty of this study was the use of a recombinant glycosidase for the selective elimination of the isomer. The isolated ginsenoside Rb3 can be used in further pharmaceutical studies. Conclusions: Herein, we demonstrated a novel enzymatic elimination-combined purification method for the chromatographic purification of ginsenoside Rb3. This method can also be applied to purify other isomeric glycoconjugates in mixtures.
Keywords
Enzymatic elimination; Ginsenoside $Rb_3$; Isomer; Purification; Transformation;
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