The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro (Colocasia esculenta Schott) in Korea

  • Kim, Min-Kyu (Division of Biological Environment, Kangwon National University) ;
  • Kwon, Soon-Bae (Gangwon Province Agricultural Research and Extension Servic) ;
  • Yoon, Ju-Yeon (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University) ;
  • Heo, Su-Jeong (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University) ;
  • Hong, Jeong-Ki (Gangwon Province Agricultural Research and Extension Servic) ;
  • Kim, Kyung-Hee (Gangwon Province Agricultural Research and Extension Servic) ;
  • Park, Jang-Kyung (Division of Biological Environment, Kangwon National University)
  • Published : 2004.06.01
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Abstract

A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

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References

  1. Abo EI-Nil, M. M., Zettler, F. W. and Hiebert, E. 1977. Purification, serology and some physical properties of dasheen mosaic virus. Phytopathology 67: 1145-1450
  2. Berger, P. H. and Shiel, P. J. 1998. Potyvirus isolation and RNA extraction. In: Foster, G. D., Taylor, S. C. (eds) Plant virology protocols. Humana Press, USA
  3. Brakke, M. K. and Ball, E. M. 1968. Purification and antigenicity of wheat streak mosaic virus. Phytopathology 58:963-971
  4. Chen, J., Chen, J., Chen, J. and Adams, M. J. 2001. Molecular characterisation of an isolate of Dasheen mosaic virus from Zantedeschia aethiopica in China and comparisons in the genus Potyvirus. Arch. Virol. 146: 1821-1829 https://doi.org/10.1007/s007050170068
  5. Choi, J. K, Maeda, T. and Wakimoto, S. 1977. An improved method for purification of turnip mosaic virus. Ann. Phytopathol. Soc. Jpn. 43:440-448 https://doi.org/10.3186/jjphytopath.43.440
  6. Damirdagh, I. S. and Shepherd, R. J. 1970. Purification of the tobacco etch and other viruses of the potato virus Y group. Phytopathology 60: 132-142 https://doi.org/10.1094/Phyto-60-132
  7. Delgano-Sanchez, S. and Grogan, R. G. 1966. Purification and properties of patato virus Y. Phytopathology 56: 1397-1404
  8. Kwon, S. B., Ha, J. H., Yoon, J. Y. and Ryu, K. H. 2002. Zantedeschia mosaic virus causing leaf mosaic symptom in Calla lily is a new potyvirus. Arch. Virol. 147:2281-2289 https://doi.org/10.1007/s00705-002-0899-y
  9. Lee, S. Y., Hong, J. S., Lee, J. S. and Choi, J. K. 1996. Detection of Cucumber mosaic virus by RT-PCR using a simple and rapid crude sap extraction method. Korean J. Plant Pathol. 12:432-436
  10. Li, R. H., Zettler, F. W., Hiebert, E., Purcifull, D. E. and Morales, F. J. 1999. Differentiation of dasheen mosaic potyvirus isolates based on variability in the apparent size of the capsid protein in western blots. J. phytopathology 147:359-364 https://doi.org/10.1046/j.1439-0434.1999.00095.x
  11. Li, R. H., Zettler, F. W, Purcifull, D. E. and Hiebert, E. 1998. The nucleotide sequence of the 3'-terminal region of dasheen mosaic virus (Caladium isolate) and expression of its coat protein (CP) in Escherichia coli for antiserum production. Arch. Virol.143:2461-2469 https://doi.org/10.1007/s007050050476
  12. Ohki, S. T. and Inouye, T. 1987. Use of Gelrite as a gelling agent in immunodiffusion tests for identification of plant virus antigens. Ann. Phytopath. Soc. Jpn. 53:557-561
  13. Pappu, S. S., Brand, R., Pappu, H. R., Rybicki, E. P., Gough, K H., Frenkel, M. J. and Niblett, C. L. 1993. A polymerase chain reaction method adapted for selective amplification and cloning of 3' sequences of potyviral genomes application to dasheen mosaic virus. J. Virol. Methods 41:9-20 https://doi.org/10.1016/0166-0934(93)90158-N
  14. Pappu, S. S., Pappu, H. R., Lastra, R. and Niblett, C. L. 1994a. Variability in the length of the amino terminal sequence contributes to the capsid protein diversity among dasheen mosaic potyvirus isolates. Arch. Virol. 136:407-413 https://doi.org/10.1007/BF01321069
  15. Pappu, S. S., Pappu, H. R., Rybicki, E. R. and Niblett, C. L. 1994b. Unusual amino terminal sequence characterizes the capsid protein of dasheen mosaic virus. J. Gen. Virol. 75:239-242 https://doi.org/10.1099/0022-1317-75-1-239
  16. Sambrook, J., Fritsch, E. F. and Maniatis, T. 1989. Molecular cloning: A Laboratory Manual, 2nd edn, Cold Spring Harbor Laboratory Press, New York, USA
  17. Sehgal, O. P. and Jean, J. 1970. Purification of maize dwarf mosaic virus by equilibrium centrifugation in cesium chloride. Phytopathology 60: 189-190 https://doi.org/10.1094/Phyto-60-189
  18. Shimoyama, J., Hanada, K., Tsuda, S., Karneya-Iwaki, M. and Gunji, T. 1992. Some physiochemical and serological properties of konjak mosaic virus and dasheen mosaic virus. Ann. Phytopathol. Soc. Jpn. 58:713-718 https://doi.org/10.3186/jjphytopath.58.713
  19. Zettler, F. W, Abo Ei-Nil, M. M. and Hiebert, E. 1978. Dasheen mosaic virus. CMI/AAB Descriptions of Plant Viruses, No. 191
  20. Zettler, F. W, Foxe, M. J., Hartman, R. D., Edwardson, J. R. and Christie, R. G. 1970. Filamentous viruses infecting dasheen and other araceous plants. Phytopathology 60:983-987 https://doi.org/10.1094/Phyto-60-983
  21. Zettler, F. W., Hiebert, E. and Rodoni, B. C. 1990. Dasheen mosaic potyvirus. In: Viruses of Tmpical Plants, A.A. BrUnt et al. (eds.) CAB International, Wallingford, Oxon, UK, pp. 237-240
  22. Zettler, F. W., Tsai, J. H., Faan, H. C., Ke, C. and Lu, K. C. 1987. Dasheen mosaic virus infecting taro in People's Republic of China. Plant Dis. 71 :837-839 https://doi.org/10.1094/PD-71-0837
  23. Zhang, Y-P., Uyemoto, J. K. and Kirkpatriek, B. C. 1998. A small-scale procedure for extracting nucleic acids from woody plants infected with various phytopathogens for PCR assay. J. Viml. Methods 71:45-50 https://doi.org/10.1016/S0166-0934(97)00190-0