• Korean Journal of Ecology and Environment
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• v.53 no.4
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• pp.344-354
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• 2020

Identification of the target species of 2-MIB (2-methyllisoborneol) production is crucial in the management of off-flavor problem in the freshwater system. This study was conducted to identify 2-MIB-producing Pseudanabaena strains occurring in the North Han River system using molecular genetic method. Eleven phenotypes of Pseudanabaena were isolated from several mainstream sites of the North Han River, including Sambong-ri, Joam-myun, and Lake Uiam areas. Despite of morphological similarity of the strains, the phylogenetic analysis using 16S rDNA classified them into different species with low genetic similarity (40~55%). Isolated Pseudanabaena strains were converged to four species; Pseudanabaena cinerea, P. yagii, P. mucicola, and P. redekei. Among them, the 2-MIB synthesizing gene (mibC) was detected in P. cinerea, P. yagii, and P. redekei. However, actual 2-MIB production was detected only in P. cinerea and P. redekei based on gas chromatography analysis. This study is the first report of the molecular identification of Pseudanabaena strains and their 2-MIB production potential in Korea. The results of this study provides an evidence of species diversity of Pseudanabaena occurring in the North Han River.

• Journal of Korean Society on Water Environment
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• v.37 no.5
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• pp.381-397
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• 2021

Off-flavor materials (geosmin and 2-methylisoborneol (2-MIB)) produced by microorganisms, such as, cyanobacteria and actinomycetes, cause freshwater use problems worldwide. Due to unpleasant taste and odor, these microorganisms have raised issues especially in drinking water resources. Recently, there has been increasing concern about 2-MIB and causal cyanobacteria, namely, Pseudanabaena, in Korea. However, material production and ecological dynamics remain largely unexplored. This study reviewed the distribution of Pseudanabaena, its species diversity, and the research trend of molecular ecology related to 2-MIB production in Korea. Based on published literature, we found that seven species of Pseudanabaena which include P. mucicola, P. limnetica, P. redekei, P. catenata, P. galeata, P. yagii, and P. cinerea appeared to occur in a variety of Korean water systems. All of these Pseudanabaena species were found in the North-Han River system (Lakes Soyang, Chuncheon, Uiam, and Paldang). Some of these species were also detected in other watersheds, but the precise species diversity was not identified. Species belonging to the Pseudanabaena genus are hard to classify through general microscopic alpha taxonomy, due to their very small cell size and similar morphological characters. Moreover, the potential of 2-MIB production cannot be detected by microscopic observation. Combining molecular ecological techniques, such as, environmental genomic materials (eDNA, eRNA) analyses to conventional methods could be useful to better understand the off-flavor material production and dynamics, thereby providing more efficient management strategies of freshwater systems.

• Korean Journal of Ecology and Environment
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• v.48 no.4
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• pp.263-271
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• 2015

To evaluate the relationship between dynamics of cyanobacteria and odorous compounds, a monthly monitoring of water quality and phytoplankton were conducted at the three serial lakes (Lake Ui-am, Lake Cheong-pyeong and Lake Pal-dang) in the North Han River for 11 times from May 2014 to March 2015. In the three serial lakes, phytoplankton communities showed that seasonal changes in Bacillariophyceae-Cyanophyceae-Bacillariophyceae. Anabaena and Pseudanabaena were dominant species in August and September 2014. At the same time the odors (Geosmin, 2-MIB) were also detected with high concentration. Relationship between environmental factors and cyanobacterial abundance showed a significant correlation with Anabaena circinalis and geosmin (r=0.983, p<0.01). In the case of Pseudanabaena limnetica showed a significant correlation of total nitrogen (r=0.685, p<0.01) and NO3-N (r=0.723, p<0.01). In addition, similarly Pseudanabaena limnetica and 2-MIB (r=0.717, p<0.01) was high. The odorous compounds appeared in the North Han River water were considered to be a direct relationship with cyanobacteria.

• Korean Journal of Ecology and Environment
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• v.56 no.2
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• pp.151-160
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• 2023

Cyanobacteria Pseudanabaena strains are known to produce 2-MIB(odorous material) in freshwater systems, thereby causing problems in water use. However, their physiological responses to environmental factors in relation with 2-MIB production is not well explored. This study was conducted to evaluate the effect of temperature on the growth and 2-MIB production of Pseudanabaena redekei. The experimental cyanobacteria strains were separated from the Uiam Reservoir (North Han River) and cultured in the BG-11 medium. Temperature was set to 10, 15, 20, 25, and 30℃ for the experiment, in the reflection of the seasonal water temperature variation in situ. For each temperature treatment, cyanobacterial biomass(Chl-a) and 2-MIB concentration (intra-cellular and extra-cellular fractions) were measured every 2 days for 18 days. Both maximal growth and total 2-MIB production of P. redekei appeared at 30℃. While intra-cellular 2-MIB contents were similar (26~29 ng L^-1) regardless of treated temperatures, extra-cellular 2-MIB concentration was higher only in high temperature conditions (25~30℃), indicating that the extents of 2-MIB biosynthesis and release by P. redekei vary with temperature. The 2-MIB productivity of P. redekei was much higher in low-temperature conditions (10~15℃) than high temperature conditions (25~30℃). This study demonstrated that temperature was a critical factor contributing to 2-MIB biosynthesis and its release in cell growth (r=0.605, p<0.01). These results are important to understand the dynamics of 2-MIB in the field and thereby provide basic information for managing odorous material in drinking water resources.

• Journal of Korean Society on Water Environment
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• v.35 no.2
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• pp.99-104
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• 2019

The objective of this study was to investigate the relationship between the biomass of cyanobacteria and the concentration of 2-methylisoborneol (2-MIB) in the lower Gongji stream. The investigation was done using a field study that was conducted from 2015 to 2017. The 2-MIB concentration in the lower Gongji stream ranged from 0 to 153 ng/L, while the upper stream had 0 2-MIB concentration. 3 genera (Oscillatoria, Phormidium, Pseudanabaena) of cyanobacteria were detected in the lower Gongji stream with 2-MIB concentration. Among these 3 genera, an increase in Phormidium, Pseudanabaena biomass was associated with an increase in 2-MIB concentration. Accordingly, Phormidium, Pseudanabaena were regarded as the biological source of 2-MIB in that area. In October 2017, although planktonic cyanobacteria occurred less frequently, many benthic cyanobacteria mats were observed on the surface of the water body. Therefore, the high 2-MIB concentration, which exceeded 110 ng/L, can likely be attributed to the benthic cyanobacteria. In a laboratory experiment, individual Oscillatoria filaments were aggregated to form a colony with a higher density. This colony tended to float on the water surface. Cyanobacteria mats after floating aggregated mats were distributed in a net shape on the bottom.

• ALGAE
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• v.36 no.3
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• pp.195-206
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• 2021

Increasing inputs of dissolved organic matter (DOM) to northern lakes is resulting in 'lake browning.' Lake browning profoundly affects phytoplankton community composition by modifying two important environmental drivers-light and nutrients. The impact of increased DOM on native isolates of red and green-pigmented cyanobacteria identified as Pseudanabaena, which emerged from a Dolichospermum bloom (Dickson Lake, Algonquin Provincial Park, Ontario, Canada) in 2015, were examined under controlled laboratory conditions. The genomes were sequenced to identify phylogenetic relatedness and physiological similarities, and the physical and chemical effects of increased DOM on cellular performance and competitiveness were assessed. Our study findings were that the isolated red and green phenotypes are two distinct species belonging to the genus Pseudanabaena; that both isolates remained physiologically unaffected when grown independently under defined DOM regimes; and that neither red nor green phenotype achieved a competitive advantage when grown together under defined DOM regimes. While photosynthetic pigment diversity among phytoplankton offers niche-differentiation opportunities, the results of this study illustrate the coexistence of two distinct photosynthetic pigment phenotypes under increasing DOM conditions.

• Journal of Ecology and Environment
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• v.38 no.4
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• pp.619-627
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• 2015

Cyanobacteria were sampled at five sites in the Han River, Nakdong River, and Geum River watershed from June 2014 to May 2015 and then cultivated. Two genera and five species of the cyanobacteria were newly recorded in Korea. The newly recorded species were Limnothrix redekei, Pseudanabaena galeata, Pseudanabaena amphigranulata, Sphaerospermopsis aphanizomenoides, and Calothrix parietina. As a result, the Korean flora of the cyanobacteria now include four orders, 22 families with 73 genera, 143 species, and two varieties, giving a total of 146 taxa.

• Korean Journal of Environmental Biology
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• v.35 no.3
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• pp.296-304
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• 2017

Samples were collected between August 2016 and May 2017 at Sangju-si, Gyeongsangbuk-do, Jeju-do, and Uljin-gun, Gangwon-do. As a result, one genus and six species were newly recorded in Korea. The unrecorded indigenous genus was Borzia, and the six species were Anagnostidinema acutissimum, Komvophoron bourrellyi, Hydrocoleum stankovicii, Borzia trilocularis, Phormidium tinctorium and Pseudanabaena lohchoides. Phormidium tinctorium and Pseudanabaena lohchoides had been reported to inhabit freshwater, but in this study, these were found in brackish water. It had also been reported that B. trilocularis trichomes are composed of up to 8 cells, but in this study, a maximum of 10 cells was observed.

• Korean Journal of Environmental Biology
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• v.24 no.3
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• pp.221-229
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• 2006

The effects of ultrasonication on phytoplankton were investigated in two ponds in which physicochemical and biological water quality was similar, one as a treatment and the other as a control. The samples were collected from August 18 to September 30 in 2003. Traditional morphological analysis showed that Bacillariophyceae dominated phytoplankton community in both ponds. The abundance of Cyanophyceae was lower in the phytoplankton community of the sonicated pond than that of control pond. We used DGGE (denaturing gradient gel electrophoresis) to analyze the diversity and change of phytoplankton community in two ponds. The DGGE banding patterns of 16S rRNA gene and sequence analysis demonstrated that Oscillatoria acuminata and CFB (Cytophaga-Flavobacterium-Bacteroides) group bacterium appeared in the treated pond, and the control pond was dominated by Synechococcus sp. and Aphanizomenon flos-aquae. Especially, Pseudanabaena sp. dominated during the ultrasonic cessation in the treated pond. The DGGE profiles of 18S rRNA gene and sequence analysis showed that the treated pond was dominated by Chlamydomonas reinhardtii and the control pond by C. reinhardtii and Pteromonas protracta. In conclusion, the ultrasonication affected the reduced growth of cyanobacteria, particularly Pseudanabaena.

• Korean Journal of Ecology and Environment
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• v.51 no.4
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• pp.299-310
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• 2018

Odor compounds (geosmin, 2-MIB) have been causing problems in the Bukhan River system, but the causative organisms have not been clearly identified. To evaluate the relationship between dynamics of microbial community and odor compounds, two times monthly monitoring of water quality and microbial community from the three serial lakes (Lake Uiam, Lake Cheongpyeong and Lake Paldang) in the Bukhan River system were conducted from April to October 2017. The odor compounds were analyzed by HS-SPME analysis method using GC/MS. Bacteria communities were identified at the class level by NGS analysis. Actinobacteria and Betaproteobacteria were dominant taxon in bacteria community of three serial lakes. In the case of phytoplankton communities showed that seasonal changes by Bacillariophyceae and Cryptophyceae in spring, Cyanobacteria in summer, and Bacillariophyceae and Cryptophyceae in autumn. Dominant species was Dolichospermum (=Anabaena), Microcystis and Pseudanabaena in Bukhan River system in all study period. At the same time the odors geosmin and 2-MIB were also detected at high concentration. There is a significant positive correlation between proportion of Actinobaceria and 2-MIB concentration (r=0.491, p<0.01). In addition, proportion of cyanobacteria showed a significant correlation of geosmin (r=0.381, p<0.05) and 2-MIB (r=0.386, p<0.05) concentration. In this study, odor compounds in the Bukhan River system are considered to be a direct relationship between with Actinobacteria and cyanobacteria.