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http://dx.doi.org/10.11614/KSL.2020.53.4.344

Molecular Identification of Pseudanabaena Strains and Analysis of 2-MIB Production Potential in the North Han River System  

Kim, Keonhee (Department of Environment and Health Science, Sanghuh College of Life Sciences, Konkuk University)
Lee, Sejin (Water Quality & Environment Center, Water Resources Management Dept, Hangang River Regional Division, K-water)
Seo, Kyunghwa (Water Quality & Environment Center, Water Resources Management Dept, Hangang River Regional Division, K-water)
Hwang, Soon-Jin (Department of Environment and Health Science, Sanghuh College of Life Sciences, Konkuk University)
Publication Information
Korean Journal of Ecology and Environment / v.53, no.4, 2020 , pp. 344-354 More about this Journal
Abstract
Identification of the target species of 2-MIB (2-methyllisoborneol) production is crucial in the management of off-flavor problem in the freshwater system. This study was conducted to identify 2-MIB-producing Pseudanabaena strains occurring in the North Han River system using molecular genetic method. Eleven phenotypes of Pseudanabaena were isolated from several mainstream sites of the North Han River, including Sambong-ri, Joam-myun, and Lake Uiam areas. Despite of morphological similarity of the strains, the phylogenetic analysis using 16S rDNA classified them into different species with low genetic similarity (40~55%). Isolated Pseudanabaena strains were converged to four species; Pseudanabaena cinerea, P. yagii, P. mucicola, and P. redekei. Among them, the 2-MIB synthesizing gene (mibC) was detected in P. cinerea, P. yagii, and P. redekei. However, actual 2-MIB production was detected only in P. cinerea and P. redekei based on gas chromatography analysis. This study is the first report of the molecular identification of Pseudanabaena strains and their 2-MIB production potential in Korea. The results of this study provides an evidence of species diversity of Pseudanabaena occurring in the North Han River.
Keywords
Pseudanabaena; species diversity; 16S rDNA; mibC gene; 2-MIB; North Han River;
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