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Change of Phytoplankton Community by Ultrasonication in Eutrophic Ponds  

Ko, So-Ra (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology)
Ahn, Chi-Yong (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology)
Joung, Seung-Hyun (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology)
Kim, Hee-Sik (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology)
Oh, Hee-Mock (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology)
Publication Information
Korean Journal of Environmental Biology / v.24, no.3, 2006 , pp. 221-229 More about this Journal
Abstract
The effects of ultrasonication on phytoplankton were investigated in two ponds in which physicochemical and biological water quality was similar, one as a treatment and the other as a control. The samples were collected from August 18 to September 30 in 2003. Traditional morphological analysis showed that Bacillariophyceae dominated phytoplankton community in both ponds. The abundance of Cyanophyceae was lower in the phytoplankton community of the sonicated pond than that of control pond. We used DGGE (denaturing gradient gel electrophoresis) to analyze the diversity and change of phytoplankton community in two ponds. The DGGE banding patterns of 16S rRNA gene and sequence analysis demonstrated that Oscillatoria acuminata and CFB (Cytophaga-Flavobacterium-Bacteroides) group bacterium appeared in the treated pond, and the control pond was dominated by Synechococcus sp. and Aphanizomenon flos-aquae. Especially, Pseudanabaena sp. dominated during the ultrasonic cessation in the treated pond. The DGGE profiles of 18S rRNA gene and sequence analysis showed that the treated pond was dominated by Chlamydomonas reinhardtii and the control pond by C. reinhardtii and Pteromonas protracta. In conclusion, the ultrasonication affected the reduced growth of cyanobacteria, particularly Pseudanabaena.
Keywords
pond; DGGE (denaturing gradient gel electrophoresis); phytoplankton; ultrasonication;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 김명운, 김민호, 조장천, 김상종. 1995. Cyanobacteria의 증식 에 따른 대청호 생태계내의 생물군집 변화. 한국육수학회지. 28:1-9
2 김범철, 김호섭, 박호동, 최광순, 박종근. 1999. 국내 호수에서 발생한 남세균의 microcystin함량과 독성평가. 한국육수학회지. 32:288-294
3 정승현, 안치용, 최애란, 장감용, 오희목. 2005. 대청호에서 강우와 식물플랑크톤 군집의 관계. 환경생물. 23:57-63   과학기술학회마을
4 정 준. 1993. 한극담수조류도감. 도서출판 아카데미서적. pp.469
5 Amann RI, W Ludwig and KH Schleiffer. 1995. Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol. Rev. 59:143-169
6 Muyzer G and K Smalla. 1998. Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology. Antonie Leeuwenhoek 73:127-141   DOI   ScienceOn
7 Oh HM, SJ Lee, JH Kim, HS Kim and BD Yoon. 2001. Seasonal variation and indirect monitoring of microcystin concentrations in Daechung Reservoir, Korea. Appl. Environ. Microbiol. 67:1484-1489   DOI   ScienceOn
8 Pinhassi J, F Azam, J Hemphala, RA Long, J Martinez, UL Zweifel and A Hagstrom. 1999. Coupling between bacterioplankton species composition, population dynamics, and organic matter degradation. Aquat. Microb. Ecol. 17:13-26   DOI
9 유광일, 임병진. 1992. 팔당호에서의 동물플랑크톤 군집구조 와 생물량의 계절적 천이. 한국육수학회지. 25:89-97
10 Negro AI, CD Hoyos and JC Vega. 2000. Phytoplankton structure and dynamics in Lake Sanabria and Valparaiso reservoir (NW Spain). Hydrobiologia 424:25-37   DOI   ScienceOn
11 Felip M and J Catalan. 2000. The relationship between phytoplankton biovolume and chlorophyll in a deep oligotrophic lake: decoupling in their spatial and temporal maxima. J. Plankton Res. 22:91-105   DOI   ScienceOn
12 Ishii K and M Fukui. 2001. Optimization of annealing temperature to reduce bias caused by a primer mismatch in multitemplate PCR. Appl. Environ. Microbiol. 67:3753-3755   DOI   ScienceOn
13 D'Elia CF, PA Steudler and N Corwin, 1977. Determination of total nitrogen in aqueous sample using persulfate digestion. Limnol. Oceanogr. 22:760-764   DOI   ScienceOn
14 강 호, 나은경, 신경숙, 장제헌. 2003. 전자선조사를 이용한 부영양화 호수와 연안에서의 조류제어 가능성에 관한 연구. 대한환경공학회지. 25:1368-1374
15 Muyzer G, EC de Waal and AG Uitterlinden. 1993. Profiling of complex microbial populations by denaturing gradient gel eletrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59:695-700
16 Cottrell MT and DL Kirchman. 2000. Natural assemblages of matine proteobacteria and members of the Cytophage-Flavobacter cluster consuming low-and high-molecular-weight dissolved organic matter. Appl. Environ. Microbiol. 66:1692-1697   DOI
17 Jaspers E, K Nauhaus, H Cypionka and J Overmann. 2001. Multitude and temporal variability of ecological niches as indicated by the diversity of cultivated bacterioplankton. FEMS Microbiol. Ecol. 36: 153-164   DOI
18 홍선희, 전선옥, 안태석, 안태영. 2002. 봄철 남세균이 우점한 천호지에서 세균군집구조의 변화. 한극미생물학회지. 38:287-292
19 Muyzer G. 1999. DGGE/TGGE a method for identifying genes from natural ecosystems. Curr. Opin. Microbiol. 2:317-322   DOI   ScienceOn
20 박정아, 허건영, 이정숙, 오윤정, 김보연, 민태익, 김치경, 안종석. 2003. 김치의 저온 발효 중 미생물 변화 양상. 한국미생물학회지. 39:45-50
21 Menzel DW and N Corwin. 1965. The measurement of total phosphorus in seawater based on the liberation of organically bound fractions by persulfate oxidation. Limnol. Oceanogr. 10:280-282   DOI   ScienceOn
22 Abell GCJ and JP Bowman. 2005. Colonization and community dynamics of class Flavobacteria on diatom detritus in experimental mesocosms based on Southern Ocean seawater. FEMS Microbiol. Ecol. 53:379-391   DOI   ScienceOn
23 Covert JS and MA Moran. 2001. Molecular characterization of estuarine bacterial communities that use high- and low-molecular weight fraction of dissolved organic carbon. Aquat. Microb. Ecol. 25: 127-139   DOI   ScienceOn
24 고소라, 박성주, 안치용, 최애란, 이정숙, 김희식, 윤병대, 오희 목. 2004. DGGE를 이용한 대청호 수화 발생시기의 세 균군집 분석. 한국미생물학회지. 40:205-210
25 Crumpton WG, TM Isenhart and PD Mitchell. 1992. Nitrate and organic N analyses with second derivative spectroscopy. Limnol. Oceanogr. 37:907-913   DOI   ScienceOn
26 김 명, 전은형, 안태영. 2003. 삽교호의 세균다양성과 계통 분류학적 분석. 한국미생물학회지. 39:272-276
27 Ahn CY, MH Park, SH Joung, HS Kim, KY Jang and HM Oh. 2003. Growth inhibition of cyanobacteria by ultrasonic radiation: laboratory and enclosure studies. Environ. Sci. Technol. 37:3031-3037   DOI   ScienceOn
28 Nakayama T, S Watanabe, K Mitsui, H Uchida and I Inouye. 1996. The phylogenetic relationship between the Chlamy-domonadales and Chlorococcales inferred from 18S rDNA sequence data. Phycol. Res. 44:47-55   DOI
29 Wood LW. 1985 Chloroform-methanol extraction of chlorophyll- a. Can. J. Fish Aquat. Sci. 42:38-43   DOI
30 Felske A, ADL Akkermans and WM de Vas. 1998. Quantification of 16S rRNAs in complex bacterial communities by multiple competitive reverse transcription PCR in temperature gradient gel electrophoresis fingerprints. Appl. Environ. Microbiol. 64:4581-4587