Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Change of Phytoplankton Community by Ultrasonication in Eutrophic Ponds

부영양 연못에서 초음파 작동에 따른 식물플랑크톤의 군집 변화

  • Ko, So-Ra (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn, Chi-Yong (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Joung, Seung-Hyun (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Hee-Sik (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology) ;
  • Oh, Hee-Mock (Environmental Biotechnology laboratory, Korea Research Institute of Bioscience and Biotechnology)
  • 고소라 (한국생명공학연구원 환경생명공학연구센터) ;
  • 안치용 (한국생명공학연구원 환경생명공학연구센터) ;
  • 정승현 (한국생명공학연구원 환경생명공학연구센터) ;
  • 김희식 (한국생명공학연구원 환경생명공학연구센터) ;
  • 오희목 (한국생명공학연구원 환경생명공학연구센터)
  • Published : 2006.09.01
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Abstract

The effects of ultrasonication on phytoplankton were investigated in two ponds in which physicochemical and biological water quality was similar, one as a treatment and the other as a control. The samples were collected from August 18 to September 30 in 2003. Traditional morphological analysis showed that Bacillariophyceae dominated phytoplankton community in both ponds. The abundance of Cyanophyceae was lower in the phytoplankton community of the sonicated pond than that of control pond. We used DGGE (denaturing gradient gel electrophoresis) to analyze the diversity and change of phytoplankton community in two ponds. The DGGE banding patterns of 16S rRNA gene and sequence analysis demonstrated that Oscillatoria acuminata and CFB (Cytophaga-Flavobacterium-Bacteroides) group bacterium appeared in the treated pond, and the control pond was dominated by Synechococcus sp. and Aphanizomenon flos-aquae. Especially, Pseudanabaena sp. dominated during the ultrasonic cessation in the treated pond. The DGGE profiles of 18S rRNA gene and sequence analysis showed that the treated pond was dominated by Chlamydomonas reinhardtii and the control pond by C. reinhardtii and Pteromonas protracta. In conclusion, the ultrasonication affected the reduced growth of cyanobacteria, particularly Pseudanabaena.

초음파가 식물플랑크톤에 미치는 영향을 조사할 목적으로, 이화학적 그리고 생물학적으로 유사한 두 군데의 부영양화 연못에서 2003년 8월 18일부터 9월 30일까지 약 4일 간격으로 시료를 채취하여 실험을 실시하였다. 실험기간 동안 출현한 식물플랑크톤 군집은 규조류(Bacillariophyceae)가 거의 대부분을 차지하였으며, 남세균(Cyanophyceae)은 상대적으로 적은 비중을 차지하였다. 분자생물학적 기법중의 하나인 DGGE(denaturing gradient gel electrophoresis)를 이용하여 식물플랑크톤 군집 구조를 비교분석하였다. 16S rDNA의 DGGE profile 분석에 의하여 처리구 연못에서는 Oscillatoria acuminata를 비롯하여 CFB (Cytophaga-Flavobacterium-Bacteroides) group bacterium 등이 나타나는 것으로 확인되었으며, 초음파가 정지된 시기에 특이적으로 Pseudanabaena sp.가 나타나는 것을 확인하였다. 대조구 연못에서는 Synechococcus sp.와 Aphanizomenon flos-aquae가 출현하는 것을 확인하였다. 18S rDNA의 DGGE profile분석결과 처리구 연못에서는 Chlamydomonas rein. hardtii가 우점하는 것으로 확인되었으며, 초음파 처리에 크게 반응하지 않는 것으로 판단된다. 대조구 연못에서는 처리구 연못에서 나타난 C. reinhardtii를 포함하여 Pteromonas protracta가 출현하였다. 결과적으로 초음파는 진핵성 식물플랑크톤 보다는 원핵성 식물플랑크톤의 다양성에 더 큰 영향을 주는 것으로 판단되며, 일부 남세균(Pseudanabaena sp.)의 생장에 저해를 주는 것으로 판단된다.

Keywords

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