• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.154-162
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• 2021

L-5-methyltetrahydrofolate (5-MTHF) is one of the biological active forms of folate, which is widely used as a nutraceutical. However, low yield and serious pollution associated with the chemical synthesis of 5-MTHF hampers its sustainable supply. In this study, 5-MTHF production was improved by engineering the 5-MTHF biosynthesis pathway and NADPH supply in Lactococcus lactis for developing a green and sustainable biosynthesis approach. Specifically, overexpressing the key rate-limiting enzyme methylenetetrahydrofolate reductase led to intracellular 5-MTHF accumulation, reaching 18 ㎍/l. Next, 5-MTHF synthesis was further enhanced by combinatorial overexpression of 5-MTHF synthesis pathway enzymes with methylenetetrahydrofolate reductase, resulting in 1.7-fold enhancement. The folate supply pathway was strengthened by expressing folE encoding GTP cyclohydrolase I, which increased 5-MTHF production 2.4-fold to 72 ㎍/l. Furthermore, glucose-6-phosphate dehydrogenase was overexpressed to improve the redox cofactor NADPH supply for 5-MTHF biosynthesis, which led to a 60% increase in intracellular NADPH and a 35% increase in 5-MTHF production (97 ㎍/l). To reduce formation of the by-product 5-formyltetrahydrofolate, overexpression of 5-formyltetrahydrofolate cyclo-ligase converted 5-formyltetrahydrofolate to 5,10-methyltetrahydrofolate, which enhanced the 5-MTHF titer to 132 ㎍/l. Finally, combinatorial addition of folate precursors to the fermentation medium boosted 5-MTHF production, reaching 300 ㎍/l. To the best of our knowledge, this titer is the highest achieved by L. lactis. This study lays the foundation for further engineering of L. lactis for efficient 5-MTHF biosynthesis.

• 한국의류산업학회지
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• 제12권2호
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• pp.162-171
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• 2010

This study aims to discuss what changes Korean clothing companies and manufacturers have undergone with production facilities moving abroad due to offshore outsourcing and examine what conditions the clothing manufacturers are facing and how the clothing companies recognize those manufacturers. For these purposes, in-depth interviews were conducted with 24 firms with annual production capacity of more than 200,000 pieces among clothing brands for the domestic market, clothing exporters and promotion agencies. Those interviewed firms were the companies who were found to produce more than 200,000 pieces of clothing per year, and the interview-based survey was conducted from December 18, 2008 to January 30, 2009. The key findings from this study are as follows; first, a high percentage of interviewed firms were producing only a small number of items in Korea with a large part of their production line transferred abroad or outsourcing all of their production abroad only with their headquarters in Korea. Second, many were employing contractors rather than their own factories. Third, when asked about the wage levels of clothing manufacturers, many of the clothing companies gave responses such as: 'They are just our contractor, so we respect their decision,' 'We don't know because it's none of our business,' or 'We don't know at all.' Fourth, when asked about the work environment of clothing manufacturers, the clothing companies gave responses such as: 'We know in part,' 'To our knowledge, they conform to the Labor Standards Law,' or 'We don't know at all.' Fifth, the production line of clothing manufacturers featured straight-line systems, specialization, pairing and compounding/mixing. Sixth, it was found that clothing companies had interest in mass customization but their preparations were not satisfactory.

• International Journal of Contents
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• 제14권3호
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• pp.69-74
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• 2018

A new breed of universities equipped with student-centered education programs and advanced digital technologies is changing the face of higher education. "Flipped learning" is heralded as a new model of education, yet its effect is underexplored. The purpose of this study is to provide evaluation criteria to assess and understand the merit of student-centered education programs and apply them to actual cases. Discussion on the nature of knowledge, its production mechanism and system, and possible contribution of digital technology to user-centered programs are discussed to produce five key criteria; initiative of students, interaction in class, interaction in field, customization of courses, and automated personal service. They are applied to evaluation of Minerva and Ecole 42.

• 한국가구학회지
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• 제25권3호
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• pp.165-172
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• 2014

3D form study is one of basic subjects in industrial design education. There are an array of textbooks of visual art, design and architecture, most of which address basic geometrical form study and abstract forms. With the introduction of computerization, current trends are directed to reduce basic form education and students' participation in classes and their accomplishments. This study was intended to develop works under a theme of furniture design with concrete shapes and functions. This study focused on developing relevant process by fusing 3D form study and furniture design which fall into basic design and design studio subjects, respectively. Among 3D form studies, applied were a concept of 3D configuration that explores the relations between surface forms and 3D forms. Furniture design is a challenge to students at beginner or intermediate level in basic design education from initial devising stage to production in kind. To ease high level of difficulties at designing and producing stages, technical education was systematized in the process of conceptualizing, developing idea and production. This type of challenge was carried out during separate semesters, along with a case study done to develop different types of challenges. This study helped students to be motivated and actively participate in classes and well perform advanced form study and technical training from design to actual production.

• 수산해양교육연구
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• 제19권1호
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• pp.101-109
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• 2007

The school system of fisheries high school was proper to specialist objective school system in order to training for expert human resource development.Training a field of human resources development from fisheries high school is fallow; fisheries production, seamen's training, ship engine and refrigerator, marine electronic telecommunication and information, fisheries foods production and fisheries foods production and distribution, fishery fisheries self-management, marine distribution, management and conservation of marine environment, safety and marine prevention of disasters, apparatus of marine development, under water area development.A new department opening and each department was revised toward to department name and department character. The unit-lesson hour of curriculum according to specialist objective school system of fisheries and marine highschool was revised. professional subject 98 unit-lesson hour(52%), normality subject 90 unit-lesson hour(48%), and educational activity of professional subject 10unit-lesson hour, total training activity 10 unit-lesson hour. And the special objective school system need to revise curriculum of 208 total unit-lesson hour.

• 실천공학교육논문지
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• 제13권3호
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• pp.483-490
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• 2021

본 논문에서는 스마트팩토리 기반의 CPPS(Cyber Physical Production System) 및 VR(Virtual Reality) 기술을 활용한 스마트팩토리 통합 기술 교육 플랫폼 개발과 플랫폼을 활용한 교육 방법들을 제안하였다. 3D 디지털 트윈과 연동이 가능하며 BOP(Bill of Process) 기반의 제조 공정을 통합하는 방법을 학습할 수 있도록 플랫폼을 개발하였다. 또한 디지털 트윈은 OPC-UA 서버를 통해 메카니컬 시스템과 디지털 트윈 뿐만 아니라 가상 현실까지 연계하여 통합 스마트팩토리 기반의 교육 플랫폼을 구축하였다. 이러한 플랫폼을 기반으로 스마트팩토리 통합 플랫폼은 BOP 기반 디지털 트윈 시뮬레이션, OPC-UA 통합, MES 시스템, SCADA 시스템, VR 연동으로 스마트팩토리 통합 플랫폼의 개별 요소들을 가지도록 제안하였다.

• Journal of Microbiology and Biotechnology
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• 제24권10호
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• pp.1368-1376
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• 2014

This article focuses on the effects of glycine betaine on preventing caramelization, and increasing DCW and $\small{L}$-lysine production. The additional glycine betaine not only decreased the browning intensity (decreased 4 times), and the concentrations of 5-hydroxymethylfurfural (decreased 7.8 times) and furfural (decreased 12 times), but also increased the availability of glucose (increased 17.5%) for $\small{L}$-lysine production. The DCW and $\small{L}$-lysine production were increased by adding no more than 20 mM glycine betaine, whereas the DCW and $\small{L}$-lysine production were decreased with the reduction of pH values, although pH had a better response to prevent caramelization than did glycine betaine. For $\small{L}$-lysine production, the highest increase (40%) was observed on the media with 20 mM glycine betaine. The crucial enzymes in glycolysis and $\small{L}$-lysine biosynthesis pathway were investigated. The results indicated that additional glycine betaine increases the activity of enzymes in glycolysis, in contrast to the effect of pH. All the results indicated that glycine betaine can be used to prevent caramelization and increase the $\small{L}$-lysine production. By applying this strategy, glucose would not be have to be separated from the culture media during autoclaving so that factories can save production costs and shorten the fermentation period.

• Journal of Microbiology and Biotechnology
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• 제28권11호
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• pp.1850-1858
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• 2018

Glucosamine (GlcN) is widely used in the nutraceutical and pharmaceutical industries. Currently, GlcN is mainly produced by traditional multistep chemical synthesis and acid hydrolysis, which can cause severe environmental pollution, require a long prodution period but a lower yield. The aim of this work was to develop a whole-cell biocatalytic process for the environment-friendly synthesis of glucosamine (GlcN) from N-acetylglucosamine (GlcNAc). We constructed a recombinant Escherichia coli and Bacillus subtilis strains as efficient whole-cell biocatalysts via expression of diacetylchitobiose deacetylase ($Dac_{ph}$) from Pyrococcus furiosus. Although both strains were biocatalytically active, the performance of B. subtilis was better. To enhance GlcN production, optimal reaction conditions were found: B. subtilis whole-cell biocatalyst 18.6 g/l, temperature $40^{\circ}C$, pH 7.5, GlcNAc concentration 50 g/l and reaction time 3 h. Under the above conditions, the maximal titer of GlcN was 35.3 g/l, the molar conversion ratio was 86.8% in 3-L bioreactor. This paper shows an efficient biotransformation process for the biotechnological production of GlcN in B. subtilis that is more environmentally friendly than the traditional multistep chemical synthesis approach. The biocatalytic process described here has the advantage of less environmental pollution and thus has great potential for large-scale production of GlcN in an environment-friendly manner.

• 문화기술의 융합
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• 제4권4호
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• pp.267-272
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• 2018

오늘날은 SNS가 파급력이 가장 큰 매체로 존재하고 있다. 수많은 SNS마케팅 디자인에 노출되어있고 SNS와 포털 사이트에 광고 노출이 많아지고 있는 상황에서 다양한 디자인들이 생산되어지고 있다. 10년 전보다도 가열되고 있는 sns광고 디자인의 현황을 알아보고 홍보와 광고 디자인의 제작에 필요한 커리튤럼을 고려하여 대학에서의 진행할 교육의 중요한 부분과 현업에 사용되고 제작되어지는 현재의 방식을 도입하는 전략에 대해 연구하고자 한다. 현대 SNS마케팅 디자인은 홍보포스터, 카드뉴스, 배너 광고 등이며 광고와 접목된 콘텐츠와 다양한 형식으로 진화되고 있다. 의사소통하거나 정보를 공유하는 데 있어, 실시간 광고 제작물 작업의 중요성과 가성비 절약이 중요하다. 이를 위해서 현장에서는 질적 향상과 함께 빠른 속도의 제작 방식으로 바뀌어 나가는 상황이며, 현재에 와서는 대학 교육의 다변화가 필요하다. SNS디자인 제작교육 개발 방안을 위한 졸업생 및 디자인 작업을 할 때, 산업현장에 나아갈 때 필요한 실시간 작업이 가능한 교육 부분에 대한 연구를 진행 하였다. 이 연구를 통하여 SNS 마케팅 디자인 인력 양성과 교육 방안에 대한 고려와 실무적인 의의를 가지고자 한다.

• Nuclear Engineering and Technology
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• 제55권8호
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• pp.2723-2733
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• 2023

Transuranic nuclides (such as ²³⁸Pu, ²⁵²Cf, ²⁴⁹Bk, etc.) have a wide range of application in industry, medicine, agriculture, and other fields. However, due to the complex conversion chain and remarkable fission losses in the process of transuranic nuclides production, the generation amounts are extremely low. High flux reactor with high neutron flux and flexible irradiation channels, is regarded as the promising candidate for producing transuranic nuclides. It is of great significance to increase the conversion ratio of transuranic nuclides, resulting in higher efficiency and better economy. In this paper, we perform an optimization design evaluation of producing transuranic nuclides in high flux reactor, which includes optimization design of irradiation target and influence study of reactor core loading. It is demonstrated that the production rate increases with appropriately determined target material and target structure. The target loading scheme in the irradiation channel also has a significant influence on the production of transuranic nuclides.