• Preventive Nutrition and Food Science
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• v.7 no.2
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• pp.215-218
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• 2002

A methanol extract of Physalis angulata exhibited in vitro antibarterial activity against oral pathogens such including Streptococcus mutans and Porphyromonas gingivalis. The methanol extract of Physalis angulata was further fractionated with ethyl acetate, n-butanol and water, in which the ethyl acetate fraction exclusively showed antibacterial activity. An active antibacterial compound from the ethyl acetate fraction was purified to a single compound using silica gel column chromatography and identified as oleanolic acid by $^{13}$ C-NMR, $^1$H-NMR and EI-MS. MIC of oleanolic acid against S. mutants and p. gingivalis were determined to be 50 and 25 ug/mL, respectively. The Antibacterial activity of oleanolic acid from Physalis angulata suggested that it has potential as an anticarcinogenic and antiperiodontic ingredients in various foods and oral care products.

• Natural Product Sciences
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• v.26 no.3
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• pp.200-206
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• 2020

The ability of the total extract from Physalis angulata; three fractions after partitioning with n-hexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 - 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 ㎍/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 - 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC. The effects of compounds 2 and 4 (10 μM) on FAS and SREBP-1c expression under high glucose conditions (30 mM) in HepG2 cells were evaluated further. Both dose-dependently inhibited FAS and SREBP-1c expression as well as lipid accumulation (1 - 10 μM) were compared to high-concentration glucose control, which upregulated FAS and SREBP-1c. These results suggest that compounds 2 and 4 upregulate AMPK, suppress FAS and SREBP-1c, and have potential effects on glucose and lipid metabolism.

• Korean Journal of Plant Resources
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• v.35 no.4
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• pp.502-507
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• 2022

In the present study, plant regeneration through adventitious shoot formation from hypocotyl segments of in vitro seedlings of groundcherry (Physalis angulata L.) was investigated to determine the optimum culture conditions for highly efficient regeneration of the species. Adventitious shoots in hypocotyl segments were efficiently induced on MS media with low concentrations of BAP, specifically, with 0.5-1.0 mg/L BAP singly or in combination with 0.1-0.5 mg/L NAA. The 1.0 mg/L BAP single treatment was most effective for forming multiple adventitious shoots. When the induced shoots were transferred to the root induction media, low concentrations of NAA, IBA, and IAA enhanced the development of adventitious roots from adventitious shoots, suggesting that low concentrations of auxins were optimal for producing regenerated plantlets. The number of roots per shoot was large (> 2.0), and the root length exceeded 8.0 cm. In particular, the development and the overall shape of the roots were ideal. Furthermore, the number and length of shoots exceeded 2 and 6.0 cm, respectively. When the regenerated plantlets were transferred to compost soil, the root and shoot systems had developed well to the point that all of the regenerated plantlets acclimated successfully, resulting in normal morphology and growth characteristics, similar to those of the mother plant. Therefore, plant regeneration via adventitious shoot formation is expected to be one of the main methods for producing groundcherry on a large scale for a stable supply of the raw materials.

• Research in Plant Disease
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• v.24 no.2
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• pp.170-173
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• 2018

Three isolates of Cucumber mosaic virus isolated from Commelina communis plants showing chlorosis and mosaic were collected in Chungju and Chuncheon, Korea. To confirm genetic variation of these three isolates (CMV-Co, CMV-Co2, and CMV-Co3), we performed PCR-RFLP and sequence analysis. Sequences of coat protein genes of CMV-C0, -Co2 and -Co3 were compared with CMV-Fny and showed 96.3%, 96.3%, and 95.9% similarities, respectively. In host reactions, three CMV-Co isolates induced systemic necrosis in Cucurbita pepo unlike CMV-Fny and CMV-Co, CMV-Co2 and CMV-Co3 observed differential symptoms responses in Physalis angulata and Nicotiana rustica. These results indicated that three isolates of CMV isolated from C. communis have genetic and biological variation.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.2
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• pp.19-39
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• 2016

This study was carried out to investigated the flora of Naknam-Jeongmaek, Korea. The survey sites were Gilmajae, Mt. Muryang, Mt. Yeohang, Mt. Muhak, Mt. Cheonju and Mt. Sineo. The flora were summarized, numbering 666 taxa including 112 families, 357 genera, 574 species, 4 subspecies, 73 varieties and 15 forms. The threatened species was Aconitum austrokoreense. The rare plants were 12 taxa including Asarum maculatum, Wikstroemia ganpi, Viola albida, Tricyrtis macropoda and so forth. The Korean endemic plants were 19 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Weigela subsessilis, Hosta minor and so forth. The specific plants by floristic region were 81 taxa including 38 taxa of grade I, 19 taxa of grade II, 16 taxa of grade III, 7 taxa of grade IV and 1 taxa of grade V. The naturalized plants were 32 taxa including Rumex crispus, Physalis angulata, Erechtites hieracifolia, Taraxacum officinale and so forth. The invasive alien plants were Ambrosia artemisiifolia and Aster pilosus.

• Korean Journal of Plant Resources
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• v.27 no.6
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• pp.601-609
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• 2014

In this study, the effect of plant extract on the senescence action and cell survival rate in two types of cells, in which aging was derived by adriamycin, was analyzed to find the materials for suppressing cell senescence from natural resources. The results are as follows. For human umbilical vein endothelial cells (HUVECs), the fruit of Physalis angulata L. and the aerial part of Synurus deltoides (Aiton) Nakai showed excellent cell-senescence inhibition activities in a treatment concentration-dependent manner, demonstrating the high possibility for utilization as a material for prevention and treatment for vascular diseases. The water extract from the root of Polygonatum odoratum var. pluriflorum for variegatum Y. N. Lee showed potent cell-senescence inhibitory effect for human dermal fibroblasts (HDFs). Thus it is considered that the additional study on the plant needs for elucidating the possible utilization as material for skin health improvement.

• Research in Plant Disease
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• v.22 no.1
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• pp.55-58
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• 2016

A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showing mosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified through confirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysis of coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco species including Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. In Chenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMV-Tr1 compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5% homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP gene indicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is the first report of CMV in T. repens in Korea.

• Journal of Radiation Industry
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• v.9 no.3
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• pp.119-126
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• 2015

In Shinjeong-dong of Jeongeup, there are three government-supported research institutes and an RFT industrial complex which is currently being established. Increased human activities can affect flora and fauna as a man-made pressure onto the region. As a baseline study, status of exotic plants was investigated prior to a full operation of the RFT industrial complex. A total of 54 species and 1 variety of naturalized or introduced plants were found in the study area. Among them, three species (Ambrosia artemisifolia var. elatior, Rumex acetocella and Aster pilosus) belong to 'nuisance species', and four species(Phytolacca americana, Iopomoea hederacea, Ereechtites hieracifolia and Rudbeckia laciniata) to 'monitor species' designated by the ministry of Environment. Some of naturalized trees and plants were intentionally introduced in this area, while others naturally immigrated. Physalis angulata seemes to immigrate in the study area in the form of mixture with animal feeds as its distribution coincided with the transportation route of the animal feeds. Liquidambar styraciflua is amenable to the ecological investigation on the possible expansion of the species to the nearby Naejang National Park as its leave shape and autumn color are very similar to those of maple trees. The number of naturalized plants around the RFT industrial complex will increase with an increase in floating population, in human activities in association with constructions of factories and operations of the complex. The result of this study provides baseline data for assessing the ecological change of the region according to the operation of the RFT industrial complex.

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.311-336
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• 2020

The flora of Sejong were summarized as 531 taxa including 104 families, 315 genera, 462 species, 3 subspecies, 59 varieties and 7 form. The rare plants were 8 taxa including Nepeta cataria L. and Aristolochia ontorta Bunge. The Korea endemic plants were 9 taxa including Populus tomentiglandulosa T. B. Le and Clematis trichotoma Nakai.. The specific plants were 42 taxa including Phytolacca esculenta Van Houtte(V), Corydalis decumbens (Thunb.) Pers(IV), Poncirus trifoliata Raf(III), Pseudostellaria coreana (Nakai) Ohwi(II), Cyrtomium fortunei J.Sm.(I). The naturalized plants were 49 taxa including Sicyos angulatus L, Ambrosia trifida L, Physalis angulata L. Panicum dichotomiflorum Michx., Galinsoga parviflora Cav, and Erigeron philadelphicus L. Especially, Sicyos angulatus L., Phytolacca americana L. and Bidens frondosa L. have been spread throughout the country are required to manage. The naturalized index(NI) and urbanized index(UI) were 8.7% and 15.3%. Useful plants incuding Edible was 199 taxa, Pasture was 193 taxa, Medicinal was 178 taxa, Ornamental was 82 taxa, Timber was 25 taxa, Fiber was 18 taxa, Stain was 9 taxa. Useful plants will be important materials for plant design in recreational and healing forests.