Browse > Article
http://dx.doi.org/10.7732/kjpr.2022.35.4.502

Plant Regeneration via Adventitious Shoot Formation from Hypocotyl Explants of Groundcherry (Physalis angulata L.)  

Koh, Seok Chan (Department of Biology, Jeju National University)
Publication Information
Korean Journal of Plant Resources / v.35, no.4, 2022 , pp. 502-507 More about this Journal
Abstract
In the present study, plant regeneration through adventitious shoot formation from hypocotyl segments of in vitro seedlings of groundcherry (Physalis angulata L.) was investigated to determine the optimum culture conditions for highly efficient regeneration of the species. Adventitious shoots in hypocotyl segments were efficiently induced on MS media with low concentrations of BAP, specifically, with 0.5-1.0 mg/L BAP singly or in combination with 0.1-0.5 mg/L NAA. The 1.0 mg/L BAP single treatment was most effective for forming multiple adventitious shoots. When the induced shoots were transferred to the root induction media, low concentrations of NAA, IBA, and IAA enhanced the development of adventitious roots from adventitious shoots, suggesting that low concentrations of auxins were optimal for producing regenerated plantlets. The number of roots per shoot was large (> 2.0), and the root length exceeded 8.0 cm. In particular, the development and the overall shape of the roots were ideal. Furthermore, the number and length of shoots exceeded 2 and 6.0 cm, respectively. When the regenerated plantlets were transferred to compost soil, the root and shoot systems had developed well to the point that all of the regenerated plantlets acclimated successfully, resulting in normal morphology and growth characteristics, similar to those of the mother plant. Therefore, plant regeneration via adventitious shoot formation is expected to be one of the main methods for producing groundcherry on a large scale for a stable supply of the raw materials.
Keywords
BAP; Multiple adventitious shoots; NAA; Regenerated plantlets; Root formation;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Hsieh, W.T., K.Y. Huang, H.Y. Lin and J.G. Chung. 2006. Physalis angulate induced G2/M phase arrest in human breast cancer cells. Food Chem. Toxicol. 44:974-983.   DOI
2 Han, B.H., B.W. Yae, D.H. Goo and H.J. Yu. 2004. Micro-propagation of Philodendron wend-imbe through adventitious multi-bud cluster formation. J. Plant Biotechnol. 31(2):115-119 (in Korean).   DOI
3 Kang, I.J., Y.J. Lee and C.H. Bae. 2021. In vitro regeneration and genetic stability analysis of the regenerated green plants in Japanese blood grass (Imperata cylindrica 'Rubra'). Korean J. Plant Res. 34(2):156-165 (in Korean).   DOI
4 Kumar, O.A., S. Ramesh and S.S. Tata. 2016. In vitro micro-propagation of the medicinal plant Physalis angulata L. Not. Sci. Biol. 8(2):161-163.   DOI
5 Kusumaningtyas, R., N. Laily and P. Limandha. 2015. Potential of Ciplukan (Physalis angulata L.) as source of functional ingredient. Procedia Chem. 14:367-372.   DOI
6 Lee, S.E., J.R. Kim, H.J. Noh, G.S. Kim, J.H. Lee, J. Choi and S.Y. Kim. 2014. Screening of plants with inhibitory activity on cellular senescence. Korean J. Plant Res. 27(6):601-609.   DOI
7 Saito, A. and Y. Ide. 1985. In vitro plantlet regeneration from adventitious buds on induced cuttings of peeled twigs of Japanese white birch. J. Jpn. For. Soc. 67:282-284.
8 Mastuti, R., A. Munawarti and E.R. Firdiana. 2017. The combination effect of auxin and cytokinin on in vitro callus formation of Physalis angulata L. - A medicinal plant. AIP Conf. Proc. 1908:040007.
9 Pennazio, S. 1975. Effect of adenine and kinetin on development of carnation tips cultured in vitro. J. Hort. Sci. 50:161-164.   DOI
10 Pham, L.H., M. Bohme and I. Pinker. 2016. Solanaceae diversity in Vietnam: a preliminary taxonomic inventory for conservation and utilization. Agric. For. 62(4):45-55.
11 Samuels, J. 2015. Biodiversity of food species of the Solanaceae family: a preliminary taxonomic inventory of subfamily Solanoideae. Resources 4(2):277-322.   DOI
12 Mascarenhas, L.M.S., J.R.F.D. Santana and A.L. Brito. 2019. Micropropagation of Physalis peruviana L. Pesq. Agropec. Trop. Goiania, 49:e55603.   DOI
13 Juang, J.K., H.W. Huang, C.M. Chen and H.J. Liu. 1989. A new compound, with angulatin A, promotes type II DNA topoisomerase-mediated DNA damage. Biochem. Biophys. Research Commun. 159(3):1128-1134.   DOI
14 Lee, T.B. 2006. Coloured Flora of Korea II. Hayngmunsa, Seoul, Korea. p. 152 (in Korean).
15 Magalhaes, H.I.F., M.R. Torres, L.V. Costa-Lotufo, M.O. De Moraes, C. Pessoa, M.L. Veras and A.P.N.N. Alves. 2006. In -vitro and in-vivo antitumour activity of physalins B and D from Physalis angulata. J. Pharm. Pharmacol. 58(2):235-241.   DOI
16 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497.   DOI
17 Oh, S.J. and S.C. Koh. 2012. Adventitious shoot formation and plant regeneration from explants of Solanum nigrum L. Korean J. Plant Res. 25(2):277-284 (in Korean).   DOI
18 Apensa, V. and R. Mastuti. 2018. Effect of banana homogenate on shoot regeneration of Ciplukan (Physalis angulata L.). J. Exp. Life Sci. 8(1):53-60.   DOI
19 Ayodhyareddy, P. and P. Rupa. 2016. Ethno medicinal, phyto chemical and therapeutic importance of Physalis angulata L.: review. Int. J. Sci. Res. 5:2122-2127.
20 Figueiredo, M.C.C., A.R. Passos, F.M. Hughes, K.S.D. Santos, A.L.D. Silva and T.L. Soares. 2020. Reproductive biology of Physalis angulata L. (Solanaceae). Sci. Hortic. 267:109307.   DOI
21 Shim, K.K. and Y.M. Ha. 1997. Mass propagation of Korean native Styrax japonicus through axillary bud culture. J. Korean Soc. Hort. Sci. 38(5):575-580 (in Korean).