Browse > Article
http://dx.doi.org/10.5423/RPD.2018.24.2.170

Comparative Analysis of Genetic Variation of Cucumber Mosaic Virus from Commelina communis in Korea  

Park, Tae-Seon (Department of Applied Biology, Kangwon National University)
Hong, Jin-Sung (Department of Applied Biology, Kangwon National University)
Publication Information
Research in Plant Disease / v.24, no.2, 2018 , pp. 170-173 More about this Journal
Abstract
Three isolates of Cucumber mosaic virus isolated from Commelina communis plants showing chlorosis and mosaic were collected in Chungju and Chuncheon, Korea. To confirm genetic variation of these three isolates (CMV-Co, CMV-Co2, and CMV-Co3), we performed PCR-RFLP and sequence analysis. Sequences of coat protein genes of CMV-C0, -Co2 and -Co3 were compared with CMV-Fny and showed 96.3%, 96.3%, and 95.9% similarities, respectively. In host reactions, three CMV-Co isolates induced systemic necrosis in Cucurbita pepo unlike CMV-Fny and CMV-Co, CMV-Co2 and CMV-Co3 observed differential symptoms responses in Physalis angulata and Nicotiana rustica. These results indicated that three isolates of CMV isolated from C. communis have genetic and biological variation.
Keywords
Coat protein gene; Commelina communis; Cucumber mosaic virus; Genetic variation;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Choi, J. K., Kwon, S. B., Lee, S. Y. and Park, W. M. 1990. Some properties of two isolates of cucumber mosaic virus isolated from Aster yomena Makino and Commelina communis L. Korean J. Plant Pathol. 6: 138-143.
2 Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RT-PCR detection and identification of three species of cucumoviruses with a genus-specifics single pair of primers. J. Virol. Methods 83: 67-73.   DOI
3 Hong, J. S., Park, H. S., Ryu, K. H. and Choi, J. K. 2009. Comparative anaylysis of coat protein gene of isolates of cucumber mosaic virus isolated from pepper plants in two GMO environmental risk assessment fields. Res. Plant Dis. 15: 165-169.   DOI
4 Kim, M. K., Seo, J. K., Kwak, H. R., Kim, J. S., Kim, K. H., Cha, B. J. and Choi, H. S. 2014. Molecular genetic analysis of cucumber mosaic virus populations infecting pepper suggests unique patterns of evolution in Korea. Phytopathology 104: 993-1000.   DOI
5 Owen, J. and Palukaitis, P. 1988. Characterization of cucumber mosaic virus. I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166: 495-502.   DOI
6 Suzuki, M., Kuwata, S., Kataoka, J., Masuta, C., Nitta, N. and Takanami, Y. 1991. Functional analysis of deletion mutants of cucumber mosaic virus RNA3 using an in vitro transcription system. Virology 183: 106-113.   DOI
7 Palukaitis, P. and Garcia-Arenal, F. 2003. Cucumoviruses. Adv. Virus Res. 62: 241-323.
8 Palukaitis, P., Roossinck, M. J., Dietzgen, R. G. and Francki, R. I. B. 1992. Cucumber mosaic virus. Adv. Virus Res. 41:281-348.
9 Roossinck, M. J., Zhang, L. and Hellwald, K. H. 1999. Rearrangements in the 5'nontranslated region and phylogenetic analyses of cucumber mosaic virus RNA 3 indicate radial evolution of three subgroups. J. Virol. 73: 6752-6758.
10 Valverde, R. A. 1983. Brome mosaic virus isolates naturally infecting Commelina diffusa and C. communis. Plant Dis. 67: 1194-1196.   DOI
11 Wahyuni, W. S., Dietzgen, R. G., Hanada, K. and Francki, R. I. B. 1992. Serological and biological variation between and within subgroup I and II strains of cucumber mosaic virus. Plant Pathol. 41: 282-297.   DOI