Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Characterization of Cucumber mosaic virus Isolated from Trifolium repens in Korea

국내 토끼풀에서 분리한 Cucumber mosaic virus의 특성

  • Park, Tae Seon (Division of Bioresource Sciences, Kangwon National University) ;
  • Choi, Gug Seoun (Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Administration) ;
  • Hong, Jin Sung (Division of Bioresource Sciences, Kangwon National University)
  • 박태선 (강원대학교 생물자원과학부) ;
  • 최국선 (농촌진흥청 국립원예특작과학원 원예특작환경과) ;
  • 홍진성 (강원대학교 생물자원과학부)
  • Received : 2015.10.12
  • Accepted : 2016.01.07
  • Published : 2016.03.31
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Abstract

A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showing mosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified through confirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysis of coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco species including Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. In Chenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMV-Tr1 compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5% homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP gene indicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is the first report of CMV in T. repens in Korea.

고추포장 주변의 모자이크 및 기형의 병징을 보이는 토끼풀에서 CMV를 분리하여 CMV-Tr1으로 명명하였다. 토끼풀로부터 RT-PCR, PCR-restriction fragment length polymorphism 및 외피단백질 유전자의 염기서열 분석을 통해 CMV를 동정하였다. 기주식물 반응결과 N. benthamiana를 포함한 5종의 담배와 오이, 꽈리 및 토마토의 상엽에서 모자이크 병징을 나타내었고, 명아주와 동부에서는 접종엽에 국부괴사 병반을 나타냈다. CMV-Tr1의 외피단백질을 CMV-As와 비교한 결과 99.5%의 상동성을 보였으며, 계통분석 결과 CMV subgroup IB에 속하는 CMV의 한 계통으로 확인되었다.

Keywords

References

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