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http://dx.doi.org/10.5423/RPD.2016.22.1.55

Characterization of Cucumber mosaic virus Isolated from Trifolium repens in Korea  

Park, Tae Seon (Division of Bioresource Sciences, Kangwon National University)
Choi, Gug Seoun (Department of Horticultural Environment, National Institute of Agricultural Biotechnology, Rural Development Administration)
Hong, Jin Sung (Division of Bioresource Sciences, Kangwon National University)
Publication Information
Research in Plant Disease / v.22, no.1, 2016 , pp. 55-58 More about this Journal
Abstract
A Cucumber mosaic virus (named CMV-Tr1) isolated from the white clover (Trifolium repens) showing mosaic and malformation that found in a pepper field. Cucumber mosaic virus was identified through confirmation with PT-PCR, PCR-restriction fragment length polymorphism, and sequence analysis of coat protein (CP) gene. CMV-Tr1 mosaic symptom on the upper leaves of five tobacco species including Nicotiana benthamiana, Cucumis sativus, Physalis angulata, and Solanum lycopersicon. In Chenopodium quinoa and Vigna unguiculata the isolate showed local lesions in inoculated leaves. CMV-Tr1 compared with CMV-As in the sequence identity of CP gene. CMV-Tr1 showed 98.9% and 99.5% homologies at nucleotide and amino acid levels, respectively. Phylogenetic analysis of the CP gene indicated that CMV-Tr1 belongs to the CMV subgroup IB base on the CP. To our knowledge, this is the first report of CMV in T. repens in Korea.
Keywords
Cucumber mosaic virus; RT-PCR; Subgroup; Trifolium repens;
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